High-resolution three-dimensional hybrid MRI + low dose CT vocal tract modeling:A cadaveric pilot study

SummaryObjectivesMRI based vocal tract models have many applications in voice research and education. These models do not adequately capture bony structures (e.g. teeth, mandible), and spatial resolution is often relatively low in order to minimize scanning time. Most MRI sequences achieve 3D vocal tract coverage at gross resolutions of 2 mm3 within a scan time of <20 seconds. Computed tomography (CT) is well suited for vocal tract imaging, but is infrequently used due to the risk of ionizing radiation. In this cadaveric study, a single, extremely low-dose CT scan of the bony structures is blended with accelerated high-resolution (1 mm3) MRI scans of the soft tissues, creating a high-resolution hybrid CT-MRI vocal tract model.MethodsMinimum CT dosages were determined and a custom 16-channel airway receiver coil for accelerated high (1 mm3) resolution MRI was evaluated. A rigid body landmark based partial volume registration scheme was then applied to the images, creating a hybrid CT-MRI model that was segmented in Slicer.ResultsUltra-low dose CT produced images with sufficient quality to clearly visualize the bone, and exposed the cadaver to 0.06 mSv. This is comparable to atmospheric exposures during a round trip transatlantic flight. The custom 16-channel vocal tract coil produced acceptable image quality at 1 mm3 resolution when reconstructed from ∼6 fold undersampled data. High (1 mm3) resolution MR imaging of short (<10 seconds) sustained sounds was achieved. The feasibility of hybrid CT-MRI vocal tract modeling was successfully demonstrated using the rigid body landmark based partial volume registration scheme. Segmentations of CT and hybrid CT-MRI images provided more detailed 3D representations of the vocal tract than 2 mm3 MRI based segmentations.ConclusionsThe method described in this study indicates that high-resolution CT and MR image sets can be combined so that structures such as teeth and bone are accurately represented in vocal tract reconstructions. Such scans will aid learning and deepen understanding of anatomical features that relate to voice production, as well as furthering knowledge of the static and dynamic functioning of individual structures relating to voice production

Combined brain language connectivity and intraoperative neurophysiologic techniques in awake craniotomy for eloquent-area brain tumor resection

Speech processing can be disturbed by primary brain tumors (PBT). Improvement of presurgical planning techniques decrease neurological morbidity associated to tumor resection during awake craniotomy. The aims of this work were: 1. To perform Diffusion Kurtosis Imaging based tractography (DKI-tract) in the detection of brain tracts involved in language; 2. To investigate which factors contribute to functional magnetic resonance imaging (fMRI) maps in predicting eloquent language regional reorganization; 3. To determine the technical aspects of accelerometric (ACC) recording of speech during surgery. DKI-tracts were streamlined using a 1.5T magnetic resonance scanner. Number of tracts and fiber pathways were compared between DKI and standard Diffusion Tensor Imaging (DTI) in healthy subjects (HS) and PBT patients. fMRI data were acquired using task-specific and resting-state paradigms during language and motor tasks. After testing intraoperative fMRI’s influence on direct cortical stimulation (DCS) number of stimuli, graph-theory measures were extracted and analyzed. Regarding speech recording, ACC signals were recorded after evaluating neck positions and filter bandwidths. To test this method, language disturbances were recorded in patients with dysphonia and after applying DCS in the inferior frontal gyrus. In contrast, HS reaction time was recorded during speech execution. DKI-tract showed increased number of arcuate fascicle tracts in PBT patients. Lower spurious tracts were identified with DKI-tract. Intraoperative fMRI and DCS showed similar stimuli in comparison with DCS alone. Increased local centrality accompanied language ipsilateral and contralateral reorganization. ACC recordings showed minor artifact contamination when placed at the suprasternal notch using a 20-200 Hz filter bandwidth. Patients with dysphonia showed decreased amplitude and frequency in comparison with HS. ACC detected an additional 11% disturbances after DCS, and a shortening of latency within the presence of a loud stimuli during speech execution. This work improved current knowledge on presurgical planning techniques based on brain structural and functional neuroimaging connectivity, and speech recordingA função linguística do ser humano pode ser afetada pela presença de tumores cerebrais (TC) A melhoria de técnicas de planeamento pré-cirurgico diminui a morbilidade neurológica iatrogénica associada ao seu tratamento cirúrgico. O objetivo deste trabalho é: 1. Testar a fiabilidade da tractografia estimada por difusor de kurtose (tract-DKI), dos feixes cerebrais envolvidos na linguagem 2. Identificar os fatores que contribuem para o mapeamento linguagem por ressonância magnética funcional (RMf) na predição da neuroplasticidade. 3. Identificar aspetos técnicos do registo da linguagem por accelerometria (ACC). A DKI-tract foi estimada após realização de RM cerebral com 1.5T. O número e percurso das fibras foi avaliado. A RMf foi adquirida durante realização de tarefas linguísticas, motoras, e em repouso. Foi testada influência dos mapas de ativação calculados por RMf, no número de estímulos realizados durante a estimulação direta cortical (EDC) intraoperatória. Medidas de conectividade foram extraídas de regiões cerebrais. A posição e filtragem de sinal ACC foram estudadas após vocalização de palavras. O sinal ACC obtido em voluntários foi comparado com doentes disfónicos, após estimulação do giro inferior frontal, e após a adição de um estímulo sonoro perturbador durante vocalização. A tract-DKI estimou um elevado número de fascículos do feixe arcuato com menos falsos negativos. Os mapas linguísticos de RMf intraoperatória, não influenciou a EDC. Medidas de centralidade aumentaram após neuroplasticidade ipsilateral e contralateral. A posição supraesternal e a filtragem de sinal ACC entre 20-200Hz demonstrou menor ruido de contaminação. Este método identificou diminuição de frequência e amplitude em doentes com disfonia, 11% de erros linguísticos adicionais após estimulação e diminuição do tempo de latência quando presente o sinal sonoro perturbador. Este trabalho promoveu a utilização de novas técnicas no planeamento pré-cirúrgico do doente com tumor cerebral e alterações da linguagem através do estudo de conectividade estrutural, funcional e registo da linguagem

Synthesis of a Vocal Sound from the 3,000 year old Mummy, Nesyamun ‘True of Voice’

The sound of a 3,000 year old mummified individual has been accurately reproduced as a vowel-like sound based on measurements of the precise dimensions of his extant vocal tract following Computed Tomography (CT) scanning, enabling the creation of a 3-D printed vocal tract. By using the Vocal Tract Organ, which provides a user-controllable artificial larynx sound source, a vowel sound is synthesised which compares favourably with vowels of modern individuals

An MRI-based articulatory and acoustic study of American English liquid sounds /r/ and /l/

In American English, the liquid sounds /r/ and /l/ are the most articulatorily variable and complex sounds. They can be produced by several distinct types of tongue configurations and are the most troublesome sounds for children and nonnative English-speakers to learn. Better understanding of this many-to-one mapping between articulation and acoustics would be beneficial to other areas such as speech pathology, speaker verification, speech recognition and speech synthesis. In this dissertation, two articulatory configurations for each liquid sound were studied (a "retroflex" /r/ vs. a "bunched" /r/, and a light /l/ vs. a dark /l/). Different from previous work on liquids, finite element analysis has been performed to obtain the acoustic responses of the three-dimensional (3-D) vocal tract models, which are based on volumetric magnetic resonance (MR) imaging. Area function models were derived based on the wave propagation property inside the vocal tract. The retroflex /r/ and the bunched /r/ show similar patterns of F1-F3 but very different spacing between F4 and F5. The results from the formant acoustic sensitivity functions and simple-tube vocal tract models suggested that this F4/F5 difference can be explained largely by differences in whether the long cavity behind the palatal constriction acts as a half- or a quarter-wavelength resonator. For both the retroflex /r/ and the bunched /r/, F4 and F5 (along with F3 for the particular speakers studied in this research) come from the long back cavity. However, these formants are half wavelength resonances for the retroflex /r/, but quarter wavelength resonances for the bunched /r/. While both the dark /l/ and the light /l/ have a linguo-alveolar contact and two lateral channels, they differ in the length of the linguo-alveolar contact and in the presence of the linguopalatal contacts caused by raising the sides of the tongue. Both have similar patterns in F1-F3, but differ in the number and locations of zeros in spectrum. For the dark /l/, only one zero occurs below 6 kHz and it is produced by the cross mode posterior to the linguo-alveolar contact. For the light /l/, three zeros below 6 kHz are produced by the asymmetrical channels, the supralingual cavity and the cross mode posterior to the linguo-alveolar contact. The results from two simple vocal tract models show that the lateral channels have to be asymmetrical with an effective length between 3-6 cm to get a zero in the region of F3-F5. Based on the Buckeye database, the acoustic variability and discriminative power of liquids were studied with the mel-frequency band energy coefficients as acoustic parameter. Analysis of variance shows that the inter-speaker variability of /r/ is larger than any other phonemes except /sh/, /s/ and /zh/. On average, /r/ and /l/ have larger inter-speaker variability than any other broad phonetic class. The F-ratio averages of liquids are larger than glides, fricatives, affricates and stops, but smaller than nasals. The speaker identification experiments show that the ranking of the average discriminative power for liquids and other broad phonetic classes is: /r/ > Glides > /l/ > Affricates > Fricatives > Stops > Nasals > Vowels

Brain structure and function: a multidisciplinary pipeline to study hominoid brain evolution

To decipher the evolution of the hominoid brain and its functions, it is essential to conduct comparative studies in primates, including our closest living relatives. However, strong ethical concerns preclude in vivo neuroimaging of great apes. We propose a responsible and multidisciplinary alternative approach that links behavior to brain anatomy in non-human primates from diverse ecological backgrounds. The brains of primates observed in the wild or in captivity are extracted and fixed shortly after natural death, and then studied using advanced MRI neuroimaging and histology to reveal macro- and microstructures. By linking detailed neuroanatomy with observed behavior within and across primate species, our approach provides new perspectives on brain evolution. Combined with endocranial brain imprints extracted from computed tomographic scans of the skulls these data provide a framework for decoding evolutionary changes in hominin fossils. This approach is poised to become a key resource for investigating the evolution and functional differentiation of hominoid brains

The Digital Fish Library: Using MRI to Digitize, Database, and Document the Morphological Diversity of Fish

Museum fish collections possess a wealth of anatomical and morphological data that are essential for documenting and understanding biodiversity. Obtaining access to specimens for research, however, is not always practical and frequently conflicts with the need to maintain the physical integrity of specimens and the collection as a whole. Non-invasive three-dimensional (3D) digital imaging therefore serves a critical role in facilitating the digitization of these specimens for anatomical and morphological analysis as well as facilitating an efficient method for online storage and sharing of this imaging data. Here we describe the development of the Digital Fish Library (DFL, http://www.digitalfishlibrary.org), an online digital archive of high-resolution, high-contrast, magnetic resonance imaging (MRI) scans of the soft tissue anatomy of an array of fishes preserved in the Marine Vertebrate Collection of Scripps Institution of Oceanography. We have imaged and uploaded MRI data for over 300 marine and freshwater species, developed a data archival and retrieval system with a web-based image analysis and visualization tool, and integrated these into the public DFL website to disseminate data and associated metadata freely over the web. We show that MRI is a rapid and powerful method for accurately depicting the in-situ soft-tissue anatomy of preserved fishes in sufficient detail for large-scale comparative digital morphology. However these 3D volumetric data require a sophisticated computational and archival infrastructure in order to be broadly accessible to researchers and educators

Virtual Exploration of the Human Vocal Tract

Hypothesis: By simulating the acoustic field throughout the entire vocal tract the evolution of speech sounds within the tract can be directly and quantitatively related to physical variations in the tract geometry. This insight into speech production could then be applied to a variety of fields where the ability to alter or investigate speech characteristics in a targeted way could be useful for example in the teaching of speech science, in speech coaching, or as part of the planning of medical procedures. In this research, a bespoke acoustic simulation package has been produced using a continuous 3-dimensional Digital Waveguide Mesh (DWM) which can produce acoustic output throughout the entire simulation domain containing the tract at every time step. This package has been shown to reproduce formant frequencies for a variety of vocal tract shapes with an average mean absolute error of 10.12% at the lips, which is comparable to other research. These results have been investigated by comparing simulation output to recorded output from physical models. This simulation package has also been used to perform studies into the shifting of formant frequencies during speech sound production along the length of the tract, and into the effect on formant frequencies of the removal of geometric features of the tract such as the piriform fossae. These studies have been compared to physical internal measurements of vocal tract models from living subjects, showing preliminary agreement with further development required. A large emphasis has been placed on the accessibility of this research, with the production of several tools for visualisation of the data contained within, and with decisions made during the production of the simulation package itself