21,724 research outputs found
NDE of structural ceramics
Radiographic, ultrasonic, scanning laser acoustic microscopy (SLAM), and thermo-acoustic microscopy techniques were used to characterize silicon nitride and silicon carbide modulus-of-rupture test specimens in various stages of fabrication. Conventional and microfocus X-ray techniques were found capable of detecting minute high density inclusions in as-received powders, green compacts, and fully densified specimens. Significant density gradients in sintered bars were observed by radiography, ultrasonic velocity, and SLAM. Ultrasonic attenuation was found sensitive to microstructural variations due to grain and void morphology and distribution. SLAM was also capable of detecting voids, inclusions and cracks in finished test bars. Consideration is given to the potential for applying thermo-acoustic microscopy techniques to green and densified ceramics. The detection probability statistics and some limitations of radiography and SLAM also are discussed
Direct and dynamic detection of HIV-1 in living cells.
In basic and applied HIV research, reliable detection of viral components is crucial to monitor progression of infection. While it is routine to detect structural viral proteins in vitro for diagnostic purposes, it previously remained impossible to directly and dynamically visualize HIV in living cells without genetic modification of the virus. Here, we describe a novel fluorescent biosensor to dynamically trace HIV-1 morphogenesis in living cells. We generated a camelid single domain antibody that specifically binds the HIV-1 capsid protein (CA) at subnanomolar affinity and fused it to fluorescent proteins. The resulting fluorescent chromobody specifically recognizes the CA-harbouring HIV-1 Gag precursor protein in living cells and is applicable in various advanced light microscopy systems. Confocal live cell microscopy and super-resolution microscopy allowed detection and dynamic tracing of individual virion assemblies at the plasma membrane. The analysis of subcellular binding kinetics showed cytoplasmic antigen recognition and incorporation into virion assembly sites. Finally, we demonstrate the use of this new reporter in automated image analysis, providing a robust tool for cell-based HIV research
Confocal microscopy
Chapter focusing on confocal microscopy. A confocal microscope is one in which the illumination is confined to a small volume in the specimen, the detection is confined to the same volume and the image is built up by scanning this volume over the specimen, either by moving the beam of light over the specimen or by displacing the specimen relative to a stationary beam. The chief advantage of this type of microscope is that it gives a greatly enhanced discrimination of depth relative to conventional microscopes. Commercial systems appeared in the 1980s and, despite their high cost, the world market for them is probably between 500 and 1000 instruments per annum, mainly because of their use in biomedical research in conjunction with fluorescent labelling methods. There are many books and review articles on this subject ( e.g. Pawley ( 2006) , Matsumoto( 2002), Wilson (1990) ). The purpose of this chapter is to provide an introduction to optical and engineering aspects that may be o f interest to biomedical users of confocal microscopy
Gigantism in unique biogenic magnetite at the Paleocene-Eocene Thermal Maximum
We report the discovery of exceptionally large biogenic magnetite crystals in clay-rich sediments spanning the Paleocene-Eocene Thermal Maximum (PETM) in a borehole at Ancora, New Jersey. Aside from previously-described abundant bacterial magnetofossils, electron microscopy reveals novel spearhead-like and spindle-like magnetite up to 4 μm long and hexaoctahedral prisms up to 1.4 μm long. Similar to magnetite produced by magnetotactic bacteria, these single-crystal particles exhibit chemical composition, lattice perfection, and oxygen isotopes consistent with an aquatic origin. Electron holography indicates single-domain magnetization despite their large crystal size. We suggest that the development of a thick suboxic zone with high iron bioavailability – a product of dramatic changes in weathering and sedimentation patterns driven by severe global warming – drove diversification of magnetite-forming organisms, likely including eukaryotes
Multi-confocal Fluorescence Correlation Spectroscopy : experimental demonstration and potential applications for living cell measurements
We report, for the first time, a multi-confocal Fluorescence Correlation
Spectroscopy (mFCS) technique which allows parallel measurements at different
locations, by combining a Spatial Light Modulator (SLM), with an Electron
Multiplying-CCD camera (EM-CCD). The SLM is used to produce a series of laser
spots, while the pixels of the EM-CCD play the roles of virtual pinholes. The
phase map addressed to the SLM is calculated by using the spherical wave
approximation and makes it possible to produce several diffraction limited
laser spots, either aligned or spread over the field of view. To attain fast
enough imaging rates, the camera has been used in different acquisition modes,
the fastest of which leads to a time resolution of 100 s. We qualified the
experimental set-up by using solutions of sulforhodamine G in glycerol and
demonstrated that the observation volumes are similar to that of a standard
confocal set-up. To demonstrate that our mFCS method is suitable for
intracellular studies, experiments have been conducted on two stable cell
lines: mouse embryonic fibroblasts expressing eGFP-actin and H1299 cells
expressing the heat shock factor fusion protein HSF1-eGFP. In the first case we
could recover, by analyzing the auto-correlation curves, the diffusion constant
of G-actin within the cytoplasm, although we were also sensitive to the complex
network of interactions with F-actin. Concerning HSF1, we could clearly observe
the modifications of the number of molecules and of the HSF1 dynamics during
heat shock
Polarization-resolved extinction and scattering cross-section of individual gold nanoparticles measured by wide-field microscopy on a large ensemble
We report a simple, rapid, and quantitative wide-field technique to measure
the optical extinction and scattering
cross-section of single nanoparticles using wide-field microscopy enabling
simultaneous acquisition of hundreds of nanoparticles for statistical analysis.
As a proof of principle, we measured nominally spherical gold nanoparticles of
40\,nm and 100\,nm diameter and found mean values and standard deviations of
and consistent with previous literature.
Switching from unpolarized to linearly polarized excitation, we measured
as a function of the polarization direction, and used it to
characterize the asphericity of the nanoparticles. The method can be
implemented cost-effectively on any conventional wide-field microscope and is
applicable to any nanoparticles
Tribological and microstructural comparison of HIPped PM212 and PM212/Au self-lubricating composites
The feasibility of replacing the silver with the volumetric equivalent of gold in the chromium carbide-based self-lubricating composite PM212 (70 wt. percent NiCo-Cr3C2, 15 percent BaF2/CaF2 eutectic) was studied. The new composite, PM212/Au has the following composition: 62 wt. percent NiCo-Cr3C2, 25 percent Au, 13 percent BaF2/CaF2 eutectic. The silver was replaced with gold to minimize the potential reactivity of the composite with possible environmental contaminants such as sulfur. The composites were fabricated by hot isostatic pressing (HIPping) and machined into pin specimens. The pins were slid against nickel-based superalloy disks. Sliding velocities ranged from 0.27 to 10.0 m/s and temperatures from 25 to 900 C. Friction coefficients ranged from 0.25 to 0.40 and wear factors for the pin and disk were typically low 10(exp -5) cu mm/N-m. HIPped PM212 measured fully dense, whereas PM212/Au had 15 percent residual porosity. Examination of the microstructures with optical and scanning electron microscopy revealed the presence of pores in PM212/Au that were not present in PM212. Though the exact reason for the residual porosity in PM212/Au was not determined, it may be due to particle morphology differences between the gold and silver and their effect on powder metallurgy processing
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