Robustness of circadian clocks to daylight fluctuations: hints from the picoeucaryote Ostreococcus tauri

The development of systemic approaches in biology has put emphasis on identifying genetic modules whose behavior can be modeled accurately so as to gain insight into their structure and function. However most gene circuits in a cell are under control of external signals and thus quantitative agreement between experimental data and a mathematical model is difficult. Circadian biology has been one notable exception: quantitative models of the internal clock that orchestrates biological processes over the 24-hour diurnal cycle have been constructed for a few organisms, from cyanobacteria to plants and mammals. In most cases, a complex architecture with interlocked feedback loops has been evidenced. Here we present first modeling results for the circadian clock of the green unicellular alga Ostreococcus tauri. Two plant-like clock genes have been shown to play a central role in Ostreococcus clock. We find that their expression time profiles can be accurately reproduced by a minimal model of a two-gene transcriptional feedback loop. Remarkably, best adjustment of data recorded under light/dark alternation is obtained when assuming that the oscillator is not coupled to the diurnal cycle. This suggests that coupling to light is confined to specific time intervals and has no dynamical effect when the oscillator is entrained by the diurnal cycle. This intringuing property may reflect a strategy to minimize the impact of fluctuations in daylight intensity on the core circadian oscillator, a type of perturbation that has been rarely considered when assessing the robustness of circadian clocks

Digital phase-lock loop

An improved digital phase lock loop incorporates several distinctive features that attain better performance at high loop gain and better phase accuracy. These features include: phase feedback to a number-controlled oscillator in addition to phase rate; analytical tracking of phase (both integer and fractional cycles); an amplitude-insensitive phase extractor; a more accurate method for extracting measured phase; a method for changing loop gain during a track without loss of lock; and a method for avoiding loss of sampled data during computation delay, while maintaining excellent tracking performance. The advantages of using phase and phase-rate feedback are demonstrated by comparing performance with that of rate-only feedback. Extraction of phase by the method of modeling provides accurate phase measurements even when the number-controlled oscillator phase is discontinuously updated

Hardware simulation of KU-band spacecraft receiver and bit synchronizer, phase 2, volume 1

The acquisition behavior of the PN subsystem of an automatically acquiring spacecraft receiver was studied. A symbol synchronizer subsystem was constructed and integrated into the composite simulation of the receiver. The overall performance of the receiver when subjected to anomalies such as signal fades was evaluated. Potential problems associated with PN/carrier sweep interactions were investigated

Ultra-stable long distance optical frequency distribution using the Internet fiber network

We report an optical link of 540 km for ultrastable frequency distribution over the Internet fiber network. The stable frequency optical signal is processed enabling uninterrupted propagation on both directions. The robustness and the performance of the link are enhanced by a cost effective fully automated optoelectronic station. This device is able to coherently regenerate the return optical signal with a heterodyne optical phase locking of a low noise laser diode. Moreover the incoming signal polarization variation are tracked and processed in order to maintain beat note amplitudes within the operation range. Stable fibered optical interferometer enables optical detection of the link round trip phase signal. The phase-noise compensated link shows a fractional frequency instability in 10 Hz bandwidth of 5x10-15 at one second measurement time and 2x10-19 at 30 000 s. This work is a significant step towards a sustainable wide area ultrastable optical frequency distribution and comparison network

Time Domain Simulations of Arm Locking in LISA

Arm locking is a technique that has been proposed for reducing laser frequency fluctuations in the Laser Interferometer Space Antenna (LISA), a gravitational-wave observatory sensitive in the milliHertz frequency band. Arm locking takes advantage of the geometric stability of the triangular constellation of three spacecraft that comprise LISA to provide a frequency reference with a stability in the LISA measurement band that exceeds that available from a standard reference such as an optical cavity or molecular absorption line. We have implemented a time-domain simulation of arm locking including the expected limiting noise sources (shot noise, clock noise, spacecraft jitter noise, and residual laser frequency noise). The effect of imperfect a priori knowledge of the LISA heterodyne frequencies and the associated 'pulling' of an arm locked laser is included. We find that our implementation meets requirements both on the noise and dynamic range of the laser frequency.Comment: Revised to address reviewer comments. Accepted by Phys. Rev.

Multi-antenna GNSS Receiver Tracking Algorithm for Vehicles With Unconstrained Three-dimensional Motion

An algorithm for GNSS GPS/GLONASS receivers is presented that allows the receiver to seamlessly combine the inputs from multiple antennas during signal tracking in order to keep full sky visibility at all times. This algorithm has applications for both aeronautical and space applications in all kinds of vehicles with unconstrained three-dimensional motion capabilities: high maneuverability jet aircraft, rockets, satellites, unmanned aerial vehicles (UAVs), etc. The algorithm presented here keeps track of the received signal-to-noise ratios (SNR) and carrier phase on each of the antennas. For each set of correlation values the receiver selects the input antenna with the best SNR in order to do navigation message decoding and ranging. This fast-switching antenna selection process allows the receiver to stay synchronized with the incoming satellite signal for as long as at least one of the antennas of the set is within line-of-sight of the transmitting satellite. Distributing enough antennas throughout the fuselage of a vehicle, this algorithm will ensure that the receiver does not lose synchronization with the GNSS signals even during threedimensional maneuvers like spins and attitude changes. This algorithm was implemented on a four-antenna GNSS receiver prototype hardware, and tested using a GNSS signal simulator. During these tests the multi-antenna tracking algorithm performed successfully. The results of some of these tests are presented in this paper.Instituto de Investigaciones en Electrónica, Control y Procesamiento de Señale

Spur Reduction Techniques for Phase-Locked Loops Exploiting A Sub-Sampling Phase Detector

This paper presents phase-locked loop (PLL) reference-spur reduction design techniques exploiting a sub-sampling phase detector (SSPD) (which is also referred to as a sampling phase detector). The VCO is sampled by the reference clock without using a frequency divider and an amplitude controlled charge pump is used which is inherently insensitive to mismatch. The main remaining source of the VCO reference spur is the periodic disturbance of the VCO by the sampling at the reference frequency. The underlying VCO sampling spur mechanisms are analyzed and their effect is minimized by using dummy samplers and isolation buffers. A duty-cycle-controlled reference buffer and delay-locked loop (DLL) tuning are proposed to further reduce the worst case spur level. To demonstrate the effectiveness of the\ud proposed spur reduction techniques, a 2.21 GHz PLL is designed and fabricated in 0.18 m CMOS technology. While using a high loop-bandwidth-to-reference-frequency ratio of 1/20, the reference spur measured from 20 chips is 80 dBc. The PLL consumes 3.8 mW while the in-band phase noise is 121 dBc/Hz at 200 kHz and the output jitter integrated from 10 kHz to 100 MHz is 0.3 ps rms

A study of Radiation-Tolerant Voltage-Controlled Oscillators designs in 65 nm bulk and 28 nm FDSOI CMOS technologies

Phase-locked loop (PLL) systems are widely employed in integrated circuits for space analog devices and communications systems that operate in radiation environments, where significant perturbations, especially in terms of phase noise, can be generated due to radiation particles. Among all the blocks that form a PLL system, previous research suggests the voltage-controlled oscillator (VCO) is one of the most critical components in terms of radiation tolerance and electric performance. Ring oscillators (ROs) and LC-tank VCOs have been commonly employed in high-performance PLLs. Nevertheless, both structures have drawbacks including a limited tuning range, high sensitivity to phase noise, limited radiation tolerance, and large design areas. In order to fulfill these high-performance requirements, a current-model logic (CML) based RO-VCO is presented as a possible solution capable of reducing the limitations of the commonly used structures and exploiting their advantages. The proposed hybrid VCO model includes passive components in its design which are the key parameters that define oscillation frequency of this structure. This tunable oscillator has been designed and tested in 65nm Bulk and 28 nm Fully depleted silicon-on-insulator (FDSOI) CMOS technologies The 65nm testchip was designed to compare the behavior of the proposed CML VCO with a current-starved RO and a radiation hardened by design (RHBD) LC-tank VCO in terms of tuning range, phase noise, Single event effect (SEE) sensitivity and design area. Simulations were carried out by applying a double exponential current pulse into different sensitive nodes of the three VCOs. In addition, SEE tests were conducted using pulsed laser experiments. Simulation and test results show that a CML VCO can effectively overcome the limitations presented by a RO-VCO and LC-tank VCO, achieving a wide range of tuning, and low sensitivity to noise and SEEs without the need for a large cross-section. Further studies of the proposed CML VCO were done on 28nm FDSOI in order to reduce the leakage current and increase the switching speed. the same current-starved VCO and CML VCO were implemented on this testchip, and simulations were performed by injecting a double exponential current pulse energy into the previously defined sensitive nodes. The results show SEE sensitivity improvement without narrowing the tuning range or affecting the phase noise response

Influence of Resonances on the Noise Performance of SQUID Susceptometers

Scanning Superconducting Quantum Interference Device (SQUID) Susceptometry simultaneously images the local magnetic fields and susceptibilities above a sample with sub-micron spatial resolution. Further development of this technique requires a thorough understanding of the current, voltage, and flux ( IVΦ ) characteristics of scanning SQUID susceptometers. These sensors often have striking anomalies in their current–voltage characteristics, which we believe to be due to electromagnetic resonances. The effect of these resonances on the performance of these SQUIDs is unknown. To explore the origin and impact of the resonances, we develop a model that qualitatively reproduces the experimentally-determined IVΦ characteristics of our scanning SQUID susceptometers. We use this model to calculate the noise characteristics of SQUIDs of different designs. We find that the calculated ultimate flux noise is better in susceptometers with damping resistors that diminish the resonances than in susceptometers without damping resistors. Such calculations will enable the optimization of the signal-to-noise characteristics of scanning SQUID susceptometers

PT-symmetric cross injection dual optoelectronic oscillator

An optoelectronic oscillator (OEO) is a time delay oscillator (TDO) that uses photonics technology to provide the long delay required to generate pristine microwave carriers. Parity-time (PT) symmetry concepts applied to an OEO offer the potential to achieve combined low phase noise and high sidemode suppression. A TDO composed of a pair of identical ring resonators coupled by a 2x2 coupler is modelled, and the coupler transmission matrix required for the oscillator to be PT- symmetric is derived. In a first configuration, the coupler is interpreted as the composition of a gain/loss block and a Mach-Zehnder interferometer (MZI) block. In practice, there are excess losses that must be compensated by a special dual amplifier with saturation characteristics compatible with PT- symmetry. The PT- symmetry phase transition determined by the gain/loss and the MZI differential phase parameters is found to be global and not local in its effect on modes. This is resolved by placing a short delay line within one arm of the MZI resulting in a frequency dependent and hence local mode-selective PT- symmetry phase transition. In addition, it is demonstrated that the first configuration may be transformed into a second but equivalent configuration as a cross-injection dual TDO with imbalanced delays. The local PT- symmetry phase transition may then be understood in terms of the Vernier effect. Advantageously, the second configuration enables the special dual amplifier to be replaced by a pair of matched but otherwise independent amplifiers. Thereby, the second configuration is amenable to practical implementation as a dual OEO using standard RF-photonic and RF-electronic components. The theory is validated by complex envelope model simulations using Simulink and phase model analytic results evaluated using MATLAB. There is excellent agreement between the theoretical and simulation results.Comment: 40 pages, 13 figure