Web Service Deployment for Selecting a Right Steganography Scheme for Optimizing Both the Capacity and the Detectable Distortion

The principal objective of this effort is to organize a network facility to hide the secret information in an image folder without disturbing its originality. In the literature lot of algorithms are there to hide the information in an image file but most of it consumes high resource for completing the task which is not suitable for light weight mobile devices. Few basic algorithms like 1LSB, 2LSB and 3LSB methods in the literature are suitable for mobile devices since the computational complexity is very low. But, these methods either lack in maintaining the originality of the source image or in increasing the number of bits to be fixed. Furthermore, every algorithm in the literature has its own merits and demerits and we cannot predict which algorithm is best or worst since, based on the parameters such as size of the safety duplicate and encryption algorithm used to generate the cipher text the steganography schemes may produce best or worst result with respect to computational complexity, capacity, and detectable distortion. In our proposed work, we have developed a web service that takes cover image and plain text as the input from the clients and returns the steganoimage to the clients. The steganoimage will be generated by our proposed work by analyzing the above said parameters and by applying the right steganography scheme. The proposed work helps in reducing the detectable distortion, computational complexity of the client device, and in increasing the capacity. The experimental result says that, the proposed system performs better than the legacy schemes with respect to capacity, computational complexity, and detectable distortion. This proposed work is more useful to the client devices with very low computational resource since all the computational tasks are deployed in the server side

Screen Genealogies

Against the grain of the growing literature on screens, *Screen Genealogies* argues that the present excess of screens cannot be understood as an expansion and multiplication of the movie screen nor of the video display. Rather, screens continually exceed the optical histories in which they are most commonly inscribed. As contemporary screens become increasingly decomposed into a distributed field of technologically interconnected surfaces and interfaces, we more readily recognize the deeper spatial and environmental interventions that have long been a property of screens. For most of its history, a screen was a filter, a divide, a shelter, or a camouflage. A genealogy stressing transformation and descent rather than origins and roots emphasizes a deeper set of intersecting and competing definitions of the screen, enabling new thinking about what the screen might yet become

Manual on application of molecular tools in aquaculture and inland fisheries management. Part 2. Laboratory protocols and data analysis

The aim of this manual is to provide a comprehensive practical tool for the generation and analysis of genetic data for subsequent application in aquatic resources management in relation to genetic stock identification in inland fisheries and aquaculture. The material only covers general background on genetics in relation to aquaculture and fisheries resource management, the techniques and relevant methods of data analysis that are commonly used to address questions relating to genetic resource characterisation and population genetic analyses. No attempt is made to include applications of genetic improvement techniques e.g. selective breeding or producing genetically modified organisms (GMOs). The manual includes two ‘stand-alone’ parts, of which this is the second volume: Part 1 – Conceptual basis of population genetic approaches: will provide a basic foundation on genetics in general, and concepts of population genetics. Issues on the choices of molecular markers and project design are also discussed. Part 2 – Laboratory protocols, data management and analysis: will provide step-by-step protocols of the most commonly used molecular genetic techniques utilised in population genetics and systematic studies. In addition, a brief discussion and explanation of how these data are managed and analysed is also included. This manual is expected to enable NACA member country personnel to be trained to undertake molecular genetic studies in their own institutions, and as such is aimed at middle and higher level technical grades. The manual can also provide useful teaching material for specialised advanced level university courses in the region and postgraduate students. The manual has gone through two development/improvement stages. The initial material was tested at a regional workshop and at the second stage feedback from participants was used to improve the contents

DEVELOPMENT OF A CARBOHYDRATE MICROARRAY SYSTEM AND A MICROCANTILEVER-BASED BIOSENSOR FOR DETECTION OF TARGET BACTERIA

The increasing number of disease outbreaks results in a demand for novel pathogen detectors. Carbohydrates serving as receptors for pathogen lectins have become the focus of such research. Two primary sugars, ‡-D-mannose and ‡-L-fucose, as receptors for Escherichia coli and Pseudomonas aeruginosa, respectively, are of great interest to researchers due to their high affinity. These interactions can be studied using carbohydrate microarrays, which are also suitable platforms for detecting bacterial pathogens. In addition, carbohydrates have the potential to act as sensing molecules in microcantilever-based biosensors. The goal of this research was to design a carbohydrate microarray system to study the interactions between bacteria and their carbohydrate receptors, and to apply these results to development of a microcantilever-based biosensor. First, a carbohydrate microarray system was developed and evaluated utilizing the recognition specificity between ‡-D-mannose and type-1 fimbriae of E. coli ORN178. Chemically synthesized polysaccharides were immobilized non-covalently onto nitrocellulose membrane-coated glass slides using a contact printer. This microarray system was used to establish the optimized conditions of bacterial cultivation and hybridization for the expression of lectins. It specifically detects E. coli ORN178 with a detection limit of 104-105 cells/membrane. This binding interaction is absent when using E. coli ORN208 (a mutant of ORN178 strain expressing abnormal type-1 fimbriae), or E. coli O157:H7 as the targets. In addition, this binding interaction is abolished when pre-exposing E. coli ORN178 to free mannose. Then, the feasibility of utilizing this carbohydrate microarray system for profiling of bacteria by their carbohydrate binding specificity was investigated using eight carbohydrates and six bacterial strains. Each bacterial strain was cultivated and allowed to hybridize to the carbohydrate microarray under its optimal conditions for cell growth and lectin expression. Second, a gold-coated microcantilever-based biosensor was covalently functionalized with carbohydrates, which served as the sensing molecules, followed by hybridization with 106cells/mL or 109cells/mL E. coli strains expressing green fluorescent proteins. Fluorescence emitted from E. coli ORN178/pGREEN or E. coli ORN208/pGREEN cells of both concentrations was observed under an epi-fluorescent microscope. Statistical analysis of the resonance frequencies, measured using Polytec Micro System Analyzer (MSA)-400, of both uncoated microcantilevers and microcantilevers functionalized with carbohydrates indicated that carbohydrate molecules were uniformly coated on the surface of functionalized microcantilevers. However, there was no statistically significant evidence to conclude that these two bacterial strains bound specifically to these fabricated microcantilever surfaces, a result different from our expectation, probably due to the non-uniform binding of bacterial cells. This research represents the first step into the development of a carbohydrate microarray system for the evaluation of bacterial binding specificity to host cell surface carbohydrates and a microcantilever biosensor based on the interactions between bacterial adhesins and host cell receptors. By exploring the use of adhesin-carbohydrate binding specificities for bacteria classification, identification, and detection, this research opens the door for more investigations in the bacteria-carbohydrate interactions and in the development of diagnostic tools and biosensors that are more stable, easier to fabricate, and ones that do not require sample pre-enrichment, cell lysis, or cell staining typically required for current antibody- or nucleic acid-based bioassays

Европейский и национальный контексты в научных исследованиях

В настоящем электронном сборнике «Европейский и национальный контексты в научных исследованиях. Технология» представлены работы молодых ученых по геодезии и картографии, химической технологии и машиностроению, информационным технологиям, строительству и радиотехнике. Предназначены для работников образования, науки и производства. Будут полезны студентам, магистрантам и аспирантам университетов.=In this Electronic collected materials “National and European dimension in research. Technology” works in the fields of geodesy, chemical technology, mechanical engineering, information technology, civil engineering, and radio-engineering are presented. It is intended for trainers, researchers and professionals. It can be useful for university graduate and post-graduate students

Portmerion, Proportion and Perspective

The holiday village of Portmerion was created by Bertram Clough Williams-Ellis (1883 1978) over a period of fifty-one years, starting in 1926. It was grade II listed in 1971. However, Portmerion has become a part of western popular culture rather than of mainstream architectural history. Its use as the setting for the cult 1967 television series “The Prisoner” ensures continued worldwide interest and a constant stream of visitors. Williams Ellis’ design methods were empirical, initial designs being adjusted by eye on site in close collaboration with trusted builders. This paper analyses the development of Portmerion as a gesamtkunstwerk; considering the experience of movement through the village as a dynamic composition of shifting vistas, focussing the visitor on a series of constructed views. Through this analysis, Portmerion is revealed as both a manifestation of the architecture of pleasure and an exercise in the pleasure of architecture

2008 IMSAloquium, Student Investigation Showcase

Marking its twentieth year, IMSA’s Student Inquiry and Research Program (SIR) is a powerful expression of the Academy’s mission, “to ignite and nurture creative ethical minds that advance the human condition.”https://digitalcommons.imsa.edu/archives_sir/1000/thumbnail.jp

The zebrafish as a novel model for the in vivo study of Toxoplasma gondii replication and interaction with macrophages.

Toxoplasma gondii is an obligate intracellular parasite capable of invading any nucleated cell. Three main clonal lineages (type I, II, III) exist and murine models have driven the understanding of general and strain-specific immune mechanisms underlying Toxoplasma infection. However, murine models are limited for studying parasite-leukocyte interactions in vivo, and discrepancies exist between cellular immune responses observed in mouse versus human cells. Here, we developed a zebrafish infection model to study the innate immune response to Toxoplasma in vivo By infecting the zebrafish hindbrain ventricle, and using high-resolution microscopy techniques coupled with computer vision-driven automated image analysis, we reveal that Toxoplasma invades brain cells and replicates inside a parasitophorous vacuole to which type I and III parasites recruit host cell mitochondria. We also show that type II and III strains maintain a higher infectious burden than type I strains. To understand how parasites are cleared in vivo, we further analyzed Toxoplasma-macrophage interactions using time-lapse microscopy and three-dimensional correlative light and electron microscopy (3D CLEM). Time-lapse microscopy revealed that macrophages are recruited to the infection site and play a key role in Toxoplasma control. High-resolution 3D CLEM revealed parasitophorous vacuole breakage in brain cells and macrophages in vivo, suggesting that cell-intrinsic mechanisms may be used to destroy the intracellular niche of tachyzoites. Together, our results demonstrate in vivo control of Toxoplasma by macrophages, and highlight the possibility that zebrafish may be further exploited as a novel model system for discoveries within the field of parasite immunity.This article has an associated First Person interview with the first author of the paper