Fuzzy-Logic Based Detection and Characterization of Junctions and Terminations in Fluorescence Microscopy Images of Neurons

Digital reconstruction of neuronal cell morphology is an important step toward understanding the functionality of neuronal networks. Neurons are tree-like structures whose description depends critically on the junctions and terminations, collectively called critical points, making the correct localization and identification of these points a crucial task in the reconstruction process. Here we present a fully automatic method for the integrated detection and characterization of both types of critical points in fluorescence microscopy images of neurons. In view of the majority of our current studies, which are based on cultured neurons, we describe and evaluate the method for application to two-dimensional (2D) images. The method relies on directional filtering and angular profile analysis to extract essential features about the main streamlines at any location in an image, and employs fuzzy logic with carefully designed rules to reason about the feature values in order to make well-informed decisions about the presence of a critical point and its type. Experiments on simulated as well as real images of neurons demonstrate the detection performance of our method. A comparison with the output of two existing neuron reconstruction methods reveals that our method achieves substantially higher detection rates and could provide beneficial information to the reconstruction process

Organization of the dorsal lateral geniculate nucleus in the mouse

AbstractThe dorsal lateral geniculate nucleus (dLGN) of the thalamus is the principal conduit for visual information from retina to visual cortex. Viewed initially as a simple relay, recent studies in the mouse reveal far greater complexity in the way input from the retina is combined, transmitted, and processed in dLGN. Here we consider the structural and functional organization of the mouse retinogeniculate pathway by examining the patterns of retinal projections to dLGN and how they converge onto thalamocortical neurons to shape the flow of visual information to visual cortex.</jats:p

Neuropathy Classification of Corneal Nerve Images Using Artificial Intelligence

Nerve variations in the human cornea have been associated with alterations in the neuropathy state of a patient suffering from chronic diseases. For some diseases, such as diabetes, detection of neuropathy prior to visible symptoms is important, whereas for others, such as multiple sclerosis, early prediction of disease worsening is crucial. As current methods fail to provide early diagnosis of neuropathy, in vivo corneal confocal microscopy enables very early insight into the nerve damage by illuminating and magnifying the human cornea. This non-invasive method captures a sequence of images from the corneal sub-basal nerve plexus. Current practices of manual nerve tracing and classification impede the advancement of medical research in this domain. Since corneal nerve analysis for neuropathy is in its initial stages, there is a dire need for process automation. To address this limitation, we seek to automate the two stages of this process: nerve segmentation and neuropathy classification of images. For nerve segmentation, we compare the performance of two existing solutions on multiple datasets to select the appropriate method and proceed to the classification stage. Consequently, we approach neuropathy classification of the images through artificial intelligence using Adaptive Neuro-Fuzzy Inference System, Support Vector Machines, Naïve Bayes and k-nearest neighbors. We further compare the performance of machine learning classifiers with deep learning. We ascertained that nerve segmentation using convolutional neural networks provided a significant improvement in sensitivity and false negative rate by at least 5% over the state-of-the-art software. For classification, ANFIS yielded the best classification accuracy of 93.7% compared to other classifiers. Furthermore, for this problem, machine learning approaches performed better in terms of classification accuracy than deep learning

Artificial neural network and its applications in quality process control, document recognition and biomedical imaging

In computer-vision based system a digital image obtained by a digital camera would usually have 24-bit color image. The analysis of an image with that many levels might require complicated image processing techniques and higher computational costs. But in real-time application, where a part has to be inspected within a few milliseconds, either we have to reduce the image to a more manageable number of gray levels, usually two levels (binary image), and at the same time retain all necessary features of the original image or develop a complicated technique. A binary image can be obtained by thresholding the original image into two levels. Therefore, thresholding of a given image into binary image is a necessary step for most image analysis and recognition techniques. In this thesis, we have studied the effectiveness of using artificial neural network (ANN) in pharmaceutical, document recognition and biomedical imaging applications for image thresholding and classification purposes. Finally, we have developed edge-based, ANN-based and region-growing based image thresholding techniques to extract low contrast objects of interest and classify them into respective classes in those applications. Real-time quality inspection of gelatin capsules in pharmaceutical applications is an important issue from the point of view of industry\u27s productivity and competitiveness. Computer vision-based automatic quality inspection and controller system is one of the solutions to this problem. Machine vision systems provide quality control and real-time feedback for industrial processes, overcoming physical limitations and subjective judgment of humans. In this thesis, we have developed an image processing system using edge-based image thresholding techniques for quality inspection that satisfy the industrial requirements in pharmaceutical applications to pass the accepted and rejected capsules. In document recognition application, success of OCR mostly depends on the quality of the thresholded image. Non-uniform illumination, low contrast and complex background make it challenging in this application. In this thesis, optimal parameters for ANN-based local thresholding approach for gray scale composite document image with non-uniform background is proposed. An exhaustive search was conducted to select the optimal features and found that pixel value, mean and entropy are the most significant features at window size 3x3 in this application. For other applications, it might be different, but the procedure to find the optimal parameters is same. The average recognition rate 99.25% shows that the proposed 3 features at window size 3x3 are optimal in terms of recognition rate and PSNR compare to the ANN-based thresholding technique with different parameters presented in the literature. In biomedical imaging application, breast cancer continues to be a public health problem. In this thesis we presented a computer aided diagnosis (CAD) system for mass detection and classification in digitized mammograms, which performs mass detection on regions of interest (ROI) followed by the benign-malignant classification on detected masses. Three layers ANN with seven features is proposed for classifying the marked regions into benign and malignant and 90.91% sensitivity and 83.87% specificity is achieved that is very much promising compare to the radiologist\u27s sensitivity 75%

On the Origin of Abstraction : Real and Imaginary Parts of Decidability-Making

International audienceThe behavioral tradition has largely anchored on Simon's early conception of bounded rationality, it is important to engage more explicitly cognitive approaches particularly ones that might link to the issue of identifying novel competitive positions. The purpose of the study is to describe the cognitive processes by which decision-makers manage to work, individually or collectively, through undecidable situations and design innovatively. Most widespread models of rationality developed for preference-making and based on a real dimension should be extended for abstraction-making by adding a visible imaginary one. A development of a core analytical/conceptual apparatus is proposed to purposely account this dual form of reasoning, deductive to prove (then make) equivalence and abstractive to represent (then unmake) it. Complex numbers, comfortable to describe repetitive, expansional and superimposing phenomena (like waves, envelope of waves, interferences or holograms, etc.) appear as generalizable to cognitive processes at work when redesigning a decidable space by abstraction (like relief vision to design a missing depth dimension, Loyd's problem to design a missing degree of freedom, etc.). This theoretical breakthrough may open up vistas capacity in the fields of information systems, knowledge and decision