Effect of oleic acid supplementation on prostaglandin production in maternal endometrial and fetal allantochorion cells isolated from late gestation ewes

Elevated circulating non-esterified fatty acids including oleic acid (OA) are associated with many pregnancy related complications. Prostaglandins (PGs) play crucial roles during parturition. We investigated the effect of OA supplementation on PG production using an in vitro model of ovine placenta

Year 1 report for ‘Conserving Texas Biodiversity: Status, Trends, and Conservation Planning for Fishes of Greatest Conservation Need’

State Wildlife Grant Program, grant TX T-106-1 (CFDA# 15.634), Contract No. 459125 UTA14-001402Substantive progress was made on all major Project Activities in this first year: Activity 1. Coordinate and Facilitate Science and Conservation Actions for Conserving Texas Biodiversity - We expanded and strengthened UT-TPWD coordination, transitioning the relationship between these partners into a much more collaborative one than was previously realized. The flow of data between TPWD and the Fishes of Texas Project (supported in part by this project) has become much more bi-directional. Many newly collected TPWD specimens, agency databases, legacy data products and reports, and feedback from resource managers are now beginning to contribute substantively to growth and diversity (now including non-specimen-vouchered records) of data served through the FoTX Project’s websites. Work on cleaning and normalizing of FoTX’s online specimen-vouchered database continued, and the updated FoTX occurrence and distribution data are being actively used. Most recently they were used by this project, together with expert (TPWD, UT and others’) opinions, to develop recommendations on conservation status of native fishes of Texas’ Species of Greatest Conservation Need for TPWD’s consideration in anticipated updates to the Texas Conservation Action Plan. Within two months of this report, a new and substantially larger and improved version of the FoTX website/database and related collection of images, field notes, and ancillary datasets, will be formally announced. Activity 2. Identify Priority Geographic Management Units for Conserving Fishes of Greatest Conservation Need - We used FoTX data in a systematic conservation area prioritization analysis to identify Native Fish Conservation Areas (NFCAs) for large portions of Texas where such comprehensive planning had not been previously carried out. Updated and new FoTX data for all Texas fish Species of Greatest Conservation Need (SGCN) were used in production of newly improved Species Distribution Models for input into this planning process, and the results of the planning exercise have already been integrated by TPWD into management prioritizations of both those species and the resultant NFCAs. Activity 3. Develop Monitoring and Conservation Plans for Native Fish Conservation Areas - Monitoring and conservation plans were delivered to TPWD for all NFCAs identified in Activity 2. Activity 4. Conduct Field-Based Surveys Detailed Biodiversity Assessments (i.e. Bioblitzing), and Citizen-Based Monitoring - Field surveys with detailed biodiversity assessments (“bioblitzes”) and citizen-based monitoring were conducted in three areas selected collaboratively by TPWD and FoTX Project staff from within the identified NFCAs: Nueces River headwaters, Big Cypress Bayou basin, and Village Creek basin. Along with this field effort, FoTX Project staff developed and circulated guidelines and best practices, and provided training for citizen-based monitoring that leverages iNaturalist for capture and reporting of photo-vouchered occurrence records in ways that will help assure scientifically useful data are obtained. All specimens acquired during these field efforts, and from many other routine specimen acquisitions from across the state (1845 total records/jars of specimens), were cataloged in the UT Fish Collection database. From there, these new records will soon be fed into GBIF, VertNet, FishNet2 and other major online data aggregators, including the online Fishes of Texas database.Texas Parks and Wildlife Department; U.S. Fish and Wildlife ServiceIntegrative Biolog

Bitopic binding mode of an M1 muscarinic acetylcholine receptor agonist associated with adverse clinical trial outcomes

The realisation of the therapeutic potential of targeting the M1 muscarinic acetylcholine receptor (M1 mAChR) for the treatment of cognitive decline in Alzheimer's disease has prompted the discovery of M1 mAChR ligands showing efficacy in alleviating cognitive dysfunction in both rodents and humans. Among these is GSK1034702, described previously as a potent M1 receptor allosteric agonist, which showed pro-cognitive effects in rodents and improved immediate memory in a clinical nicotine withdrawal test but induced significant side-effects. Here we provide evidence using ligand binding, chemical biology and functional assays to establish that rather than the allosteric mechanism claimed, GSK1034702 interacts in a bitopic manner at the M1 mAChR such that it can concomitantly span both the orthosteric and an allosteric binding site. The bitopic nature of GSK1034702 together with the intrinsic agonist activity and a lack of muscarinic receptor subtype selectivity reported here, all likely contribute to the adverse effects of this molecule in clinical trials. We conclude that these properties, whilst imparting beneficial effects on learning and memory, are undesirable in a clinical candidate due to the likelihood of adverse side effects. Rather, our data supports the notion that "pure" positive allosteric modulators showing selectivity for the M1 mAChR with low levels of intrinsic activity would be preferable to provide clinical efficacy with low adverse responses

Effects of cell elasticity on the migration behavior of a monolayer of motile cells: Sharp Interface Model

In order to study the effect of cell elastic properties on the behavior of assemblies of motile cells, this paper describes an alternative to the cell phase field (CPF) \cite{Palmieri2015} we have previously proposed. The CPF is a multi-scale approach to simulating many cells which tracked individual cells and allowed for large deformations. Though results were largely in agreement with experiment that focus on the migration of a soft cancer cell in a confluent layer of normal cells \cite{Lee2012}, simulations required large computing resources, making more detailed study unfeasible. In this work we derive a sharp interface limit of CPF, including all interactions and parameters. This new model offers over $200$ fold speedup when compared to our original CPF implementation. We demonstrate that this model captures similar behavior and allows us to obtain new results that were previously intractable. We obtain the full velocity distribution for a large range of degrees of confluence, $\rho$, and show regimes where its tail is heavier and lighter than a normal distribution. Furthermore, we fully characterize the velocity distribution with a single parameter, and its dependence on $\rho$ is fully determined. Finally, cell motility is shown to linearly decrease with increasing $\rho$, consistent with previous theoretical results

Water Planning In Alcobaça Cistercian Lands / O Ordenamento Hidráulico no Território Cisterciense de Alcobaça

This paper concerns the main domain (coutos) of the Cistercian Abbey of Alcobaça (central Portugal), founded in 1153. It shows the involvement of the monks in shaping hydraulic landscapes along time. This monastic territory is limited westwards by the Atlantic ocean with a cliff coast indented by two large gulfs, the former Pederneira and Alfeizerão lagoons, sanded up presently. These landscapes have been consolidated along with the monks’ intervention in the hydrographic plan, particularly through a network of canals, the types of which can be summarized as follows: canals for water conveyance and evacuation, to and from the abbey buildings; canals related with water-powered engines as grain-, oil-, saw- and fulling-mills, forges and other industrial devices; canals consequent to the diversion of rivers and streams with two main purposes: to drain the fields in order to improve the marshes for agricultural use, and to irrigate cultures

Heritage entrepreneurship. Agency-driven promotion of the Mediterranean diet in Spain.

This article explores the role of the agency in the social process that constitutes cultural heritage. By introducing the concept of heritage entrepreneurship to explain the conversion of cultural elements into heritage, we discuss the case of the Mediterranean diet (MD) in Spain. We explore the role of an expert NGO in the recent inclusion of the MD in the UNESCO Representative List of the intangible cultural heritage of Humanity. Empirical evidence is presented for two basic patterns of heritage entrepreneurship, namely the construction and promotion of cultural heritage. First, we show how the community-heritage narrative is constructed in the official nomination file of the MD. Second, we analyse how businesses, governments and researchers constitute a specific heritage entrepreneur. We argue that the promotion of the MD as cultural heritage makes ordinary food different, both qualitatively (healthy and sustainable) and culturally (Mediterranean and traditional). We then look at the specific political, economic and scientific value of such a difference and its uses in Spain