Prevalence of Inherited Hemoglobin Disorders and Relationships with Anemia and Micronutrient Status among Children in Yaoundé and Douala, Cameroon.

Information on the etiology of anemia is necessary to design effective anemia control programs. Our objective was to measure the prevalence of inherited hemoglobin disorders (IHD) in a representative sample of children in urban Cameroon, and examine the relationships between IHD and anemia. In a cluster survey of children 12-59 months of age (n = 291) in Yaoundé and Douala, we assessed hemoglobin (Hb), malaria infection, and plasma indicators of inflammation and micronutrient status. Hb S was detected by HPLC, and α⁺thalassemia (3.7 kb deletions) by PCR. Anemia (Hb < 110 g/L), inflammation, and malaria were present in 45%, 46%, and 8% of children. A total of 13.7% of children had HbAS, 1.6% had HbSS, and 30.6% and 3.1% had heterozygous and homozygous α⁺thalassemia. The prevalence of anemia was greater among HbAS compared to HbAA children (60.3 vs. 42.0%, p = 0.038), although mean Hb concentrations did not differ, p = 0.38). Hb and anemia prevalence did not differ among children with or without single gene deletion α⁺thalassemia. In multi-variable models, anemia was independently predicted by HbAS, HbSS, malaria, iron deficiency (ID; inflammation-adjusted ferritin <12 µg/L), higher C-reactive protein, lower plasma folate, and younger age. Elevated soluble transferrin receptor concentration (>8.3 mg/L) was associated with younger age, malaria, greater mean reticulocyte counts, inflammation, HbSS genotype, and ID. IHD are prevalent but contribute modestly to anemia among children in urban Cameroon

Streaming Kernelization

Kernelization is a formalization of preprocessing for combinatorially hard problems. We modify the standard definition for kernelization, which allows any polynomial-time algorithm for the preprocessing, by requiring instead that the preprocessing runs in a streaming setting and uses $\mathcal{O}(poly(k)\log|x|)$ bits of memory on instances $(x,k)$. We obtain several results in this new setting, depending on the number of passes over the input that such a streaming kernelization is allowed to make. Edge Dominating Set turns out as an interesting example because it has no single-pass kernelization but two passes over the input suffice to match the bounds of the best standard kernelization

The insect pathogen Serratia marcescens Db10 uses a hybrid non-ribosomal peptide synthetase-polyketide synthase to produce the antibiotic althiomycin

There is a continuing need to discover new bioactive natural products, such as antibiotics, in genetically-amenable micro-organisms. We observed that the enteric insect pathogen, Serratia marcescens Db10, produced a diffusible compound that inhibited the growth of Bacillis subtilis and Staphyloccocus aureus. Mapping the genetic locus required for this activity revealed a putative natural product biosynthetic gene cluster, further defined to a six-gene operon named alb1-alb6. Bioinformatic analysis of the proteins encoded by alb1-6 predicted a hybrid non-ribosomal peptide synthetase-polyketide synthase (NRPS-PKS) assembly line (Alb4/5/6), tailoring enzymes (Alb2/3) and an export/resistance protein (Alb1), and suggested that the machinery assembled althiomycin or a related molecule. Althiomycin is a ribosome-inhibiting antibiotic whose biosynthetic machinery had been elusive for decades. Chromatographic and spectroscopic analyses confirmed that wild type S. marcescens produced althiomycin and that production was eliminated on disruption of the alb gene cluster. Construction of mutants with in-frame deletions of specific alb genes demonstrated that Alb2-Alb5 were essential for althiomycin production, whereas Alb6 was required for maximal production of the antibiotic. A phosphopantetheinyl transferase enzyme required for althiomycin biosynthesis was also identified. Expression of Alb1, a predicted major facilitator superfamily efflux pump, conferred althiomycin resistance on another, sensitive, strain of S. marcescens. This is the first report of althiomycin production outside of the Myxobacteria or Streptomyces and paves the way for future exploitation of the biosynthetic machinery, since S. marcescens represents a convenient and tractable producing organism

Polish yers revisited

It is common in linguistic research to attempt a unified analysis for similar patterns in related languages. In this paper, I argue that to do so for Polish and Russian vowel alternations would be a mistake. Although they share some notable phonological properties, they differ in their prevalence and their extensibility. I present an account of Polish under which vowel alternations are unexceptional, and exceptional blocking of alternation is achieved with lexically indexed constraints. This is the complement of Gouskova's (2012) account of Russian, which I argue to be desirable on the basis of novel corpus statistics from the Polish lexicon and their divergences from the trends for analogous words in Russian.En la investigació lingüística és corrent buscar una anàlisi unificada per a fenòmes similars de llengües relacionades. En aquest treball, s'argumenta que fer-ho per a les alternaces vocàliques del polonès i del rus seria un error. Encara que comparteixen propietats fonològiques notables, difereixen respecte a la seva prevalència i la seva extensibilitat. Es presenta una anàlisi del polonès en la qual les alternances vocàliques no són excepcionals i el blocatge excepcional de les alternances s'assoleix amb restriccions indexades lèxicament. Aquesta és una anàlisi complementària de la de Gouskova (2012) per al rus, que s'argumenta que és desitjable sobre la base d'una nova exploració estadística d'un corpus del lexicó polonès i les seves divergències respecte a les tendències per a mots anàlegs en rus