A portable 3D printer system for the diagnosis and treatment of multidrug-resistant bacteria

Summary: Multidrug-resistant bacteria are a major threat to human health, but broad-spectrum antibiotics are losing efficacy. There is a need to screen a given drug against a bacterial infection outside of the laboratory. To address this need, we have designed and built an inexpensive and easy-to-use 3D-printer-based system that allows easily readable quantitative tests for the performance of antibacterial drugs. The platform creates a sterile diagnostic device by using 3D printing, and the device is filled automatically with growth medium, and then antibiotics are sprayed onto the medium by ink-jet technology. The sample for testing can be introduced via a fluid port, and the printer hot bed is used to incubate the device, allowing operation in the field. Combining advantages from various established tests of antibacterial performance, this allows the comparison of a specific antibiotics and bacteria. Also, this system can create and test several antibiotic formulations for therapeutic use, and we demonstrate this potential by investigating a mixture of pathogens that are only killed by a mixture of drugs

Micro-manufacturing : research, technology outcomes and development issues

Besides continuing effort in developing MEMS-based manufacturing techniques, latest effort in Micro-manufacturing is also in Non-MEMS-based manufacturing. Research and technological development (RTD) in this field is encouraged by the increased demand on micro-components as well as promised development in the scaling down of the traditional macro-manufacturing processes for micro-length-scale manufacturing. This paper highlights some EU funded research activities in micro/nano-manufacturing, and gives examples of the latest development in micro-manufacturing methods/techniques, process chains, hybrid-processes, manufacturing equipment and supporting technologies/device, etc., which is followed by a summary of the achievements of the EU MASMICRO project. Finally, concluding remarks are given, which raise several issues concerning further development in micro-manufacturing

Beyond 3D Printing: The New Dimensions of Additive Fabrication

Additive fabrication, often referred to as 3D printing, is the construction of objects by adding material. This stands in contrast to subtractive methods, which involve removing material by means of milling or cutting. Although additive fabrication and 3D printing are thought of as synonymous, additive fabrication encompasses a far broader range of construction, and new dimensions are on the horizon, inspiring innovation across scales and applications. For instance, can you print a full-scale building? How can we structurally engineer color and alter on the nanoscale? If trees grow additively, can biology be designed for fabrication

Design, evaluation, and control of nexus: a multiscale additive manufacturing platform with integrated 3D printing and robotic assembly.

Additive manufacturing (AM) technology is an emerging approach to creating three-dimensional (3D) objects and has seen numerous applications in medical implants, transportation, aerospace, energy, consumer products, etc. Compared with manufacturing by forming and machining, additive manufacturing techniques provide more rapid, economical, efficient, reliable, and complex manufacturing processes. However, additive manufacturing also has limitations on print strength and dimensional tolerance, while traditional additive manufacturing hardware platforms for 3D printing have limited flexibility. In particular, part geometry and materials are limited to most 3D printing hardware. In addition, for multiscale and complex products, samples must be printed, fabricated, and transferred among different additive manufacturing platforms in different locations, which leads to high cost, long process time, and low yield of products. This thesis investigates methods to design, evaluate, and control the NeXus, which is a novel custom robotic platform for multiscale additive manufacturing with integrated 3D printing and robotic assembly. NeXus can be used to prototype miniature devices and systems, such as wearable MEMS sensor fabrics, microrobots for wafer-scale microfactories, tactile robot skins, next generation energy storage (solar cells), nanostructure plasmonic devices, and biosensors. The NeXus has the flexibility to fixture, position, transport, and assemble components across a wide spectrum of length scales (Macro-Meso-Micro-Nano, 1m to 100nm) and provides unparalleled additive process capabilities such as 3D printing through both aerosol jetting and ultrasonic bonding and forming, thin-film photonic sintering, fiber loom weaving, and in-situ Micro-Electro-Mechanical System (MEMS) packaging and interconnect formation. The NeXus system has a footprint of around 4m x 3.5m x 2.4m (X-Y-Z) and includes two industrial robotic arms, precision positioners, multiple manipulation tools, and additive manufacturing processes and packaging capabilities. The design of the NeXus platform adopted the Lean Robotic Micromanufacturing (LRM) design principles and simulation tools to mitigate development risks. The NeXus has more than 50 degrees of freedom (DOF) from different instruments, precise evaluation of the custom robots and positioners is indispensable before employing them in complex and multiscale applications. The integration and control of multi-functional instruments is also a challenge in the NeXus system due to different communication protocols and compatibility. Thus, the NeXus system is controlled by National Instruments (NI) LabVIEW real-time operating system (RTOS) with NI PXI controller and a LabVIEW State Machine User Interface (SMUI) and was programmed considering the synchronization of various instruments and sequencing of additive manufacturing processes for different tasks. The operation sequences of each robot along with relevant tools must be organized in safe mode to avoid crashes and damage to tools during robots’ motions. This thesis also describes two demonstrators that are realized by the NeXus system in detail: skin tactile sensor arrays and electronic textiles. The fabrication process of the skin tactile sensor uses the automated manufacturing line in the NeXus with pattern design, precise calibration, synchronization of an Aerosol Jet printer, and a custom positioner. The fabrication process for electronic textiles is a combination of MEMS fabrication techniques in the cleanroom and the collaboration of multiple NeXus robots including two industrial robotic arms and a custom high-precision positioner for the deterministic alignment process

Rapid annealing of Perovskite solar cell thin film materials through intense pulse light.

Perovskite solar cells (PSCs) have garnered a great attention due to their rapid efficiency improvement using cheap and solution processable materials that can be adapted for scalable high-speed automated manufacturing. Thin film perovskite photovoltaics (PVs) are typically fabricated in an inert environment, such as nitrogen glovebox, through a set of deposition and annealing steps, each playing a significant role on the power conversion efficiency (PCE), reproducibility, and stability of devices. However, atmospheric processing of PSCs would achieve lucrative commercialization. Therefore, it is necessary to utilize materials and methods that enable successful fabrication of efficient PSCs in the ambient environment. The lab scale experiments have been dominated using deposition methods, such as spin-coating or thermal evaporation in vacuum, which are not adaptable for automation; hence, taking advantage of scalable deposition methods, such as inkjet printing, is necessary for automation. Besides deposition, post process annealing is a pivotal aspect which crystallizes the thin film materials and determines the performance and stability of PSCs. Therefore, it is necessary to further investigate this step and develop new methods and utilize potential materials vi that are amenable for scalable, high-throughput, and cost-effective automated manufacturing of PSCs. Conventional methods have successfully resulted in efficient labscale PSCs using prolong and high temperature annealing; however, industrialization requires rapid annealing methods that allow for scalable, high-speed, and cost-effective manufacturing of efficient PSCs in the ambient environment. Intense pulse light is a rapid annealing method (IPL) that allows for the lucrative, scalable, and high throughput atmospheric processing of PSCs; thus, it is necessary to study the photothermal impact on the morphology and phase evolution of the thin film materials and develop ambient processable precursors that yield efficient perovskite modules. IPL exerts intermittent millisecond(s) duration flashes carrying energetic photons to anneal the material, and the parameters of flash energy, duration, count, and interval time between flashes determine the annealing extent and affect the PV performance of PSCs. This dissertation investigates the impact of these parameters on the morphology, phase change, and conductivity of the potential PSC thin films using various material characterization techniques of scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), photoluminescence (PL), impedance spectroscopy (IS), X-ray photoelectron spectroscopy (XPS), UV-Vis, as well as voltammetry, by introducing a novel additive and annealing approaches which allow for rapid fabrication of PSCs, and is applicable for rapid, cost-effective, and scalable automated fabrication of PSCs

New Manufacturing Environments with Micro- and Nanorobotics

UIDB/04647/2020 UIDP/04647/2020The convergence of nano-, bio-, information, and cognitive sciences and technologies (NBIC) is advancing continuously in many societal spheres. This also applies to the manufacturing sector, where technological transformations in robotics push the boundaries of human–machine interaction (HMI). Here, current technological advances in micro- and nanomanufacturing are accompanied by new socio-economic concepts for different sectors of the process industry. Although these developments are still ongoing, the blurring of the boundaries of HMI in processes at the micro- and nano- level can already be observed. According to the authors, these new socio-technical HMIs may lead to the development of new work environments, which can also have an impact on work organization. While there is still little empirical evidence, the following contribution focuses on the question whether the “manufacturing (or working) life” using enhancement practices pushes the boundaries of HMI and how these effects enable new modes of working in manufacturing. Issues of standardization, acceleration of processes, and order-oriented production become essential for technological innovation in this field. However, these trends tend to lead to a “manufacturing life” in work environments rather than to new modes of work in industry.publishersversionepub_ahead_of_prin

Connected healthcare: Improving patient care using digital health technologies

Now more than ever, traditional healthcare models are being overhauled with digital technologies of Healthcare 4.0 being increasingly adopted. Worldwide, digital devices are improving every stage of the patient care pathway. For one, sensors are being used to monitor patient metrics 24/7, permitting swift diagnosis and interventions. At the treatment stage, 3D printers are currently being investigated for the concept of personalised medicine by allowing patients access to on-demand, customisable therapeutics. Robots are also being explored for treatment, by empowering precision surgery or targeted drug delivery. Within medical logistics, drones are being leveraged to deliver critical treatments to remote areas, collect samples, and even provide emergency aid. To enable seamless integration within healthcare, the Internet of Things technology is being exploited to form closed-loop systems that remotely communicate with one another. This review outlines the most promising healthcare technologies and devices, their strengths, drawbacks, and scopes for clinical adoption

DEVELOPMENT OF FUNCTIONAL NANOCOMPOSITE MATERIALS TOWARDS BIODEGRADABLE SOFT ROBOTICS AND FLEXIBLE ELECTRONICS

World population is continuously growing, as well as the influence we have on the ecosystem\u2019s natural equilibrium. Moreover, such growth is not homogeneous and it results in an overall increase of older people. Humanity\u2019s activity, growth and aging leads to many challenging issues to address: among them, there are the spread of suddenly and/or chronic diseases, malnutrition, resource pressure and environmental pollution. Research in the novel field of biodegradable soft robotics and electronics can help dealing with these issues. In fact, to face the aging of the population, it is necessary an improvement in rehabilitation technologies, physiological and continuous monitoring, as well as personalized care and therapy. Also in the agricultural sector, an accurate and efficient direct measure of the plants health conditions would be of help especially in the less-developed countries. But since living beings, such as humans and plants, are constituted by soft tissues that continuously change their size and shapes, today\u2019s traditional technologies, based on rigid materials, may not be able to provide an efficient interaction necessary to satisfy these needs: the mechanical mismatch is too prohibitive. Instead, soft robotic systems and devices can be designed to combine active functionalities with soft mechanical properties that can allow them to efficiently and safely interact with soft living tissues. Soft implantable biomedical devices, smart rehabilitation devices and compliant sensors for plants are all applications that can be achieved with soft technologies. The development of sophisticated autonomous soft systems needs the integration on a unique soft body or platform of many functionalities (such as mechanical actuation, energy harvesting, storage and delivery, sensing capabilities). A great research interest is recently arising on this topic, but yet not so many groups are focusing their efforts in the use of natural-derived and biodegradable raw materials. In fact, resource pressure and environmental pollution are becoming more and more critical problems. It should be completely avoided the use of in exhaustion, pollutant, toxic and non-degradable resources, such as lithium, petroleum derivatives, halogenated compounds and organic solvents. So-obtained biodegradable soft systems and devices could then be manufactured in high number and deployed in the environment to fulfil their duties without the need to recover them, since they can safely degrade in the environment. The aim of the current Ph.D. project is the use of natural-derived and biodegradable polymers and substances as building blocks for the development of smart composite materials that could operate as functional elements in a soft robotic system or device. Soft mechanical properties and electronic/ionic conductive properties are here combined together within smart nanocomposite materials. The use of supersonic cluster beam deposition (SCBD) technique enabled the fabrication of cluster-assembled Au electrodes that can partially penetrate into the surface of soft materials, providing an efficient solution to the challenge of coupling conductive metallic layers and soft deformable polymeric substrates. In this work, cellulose derivatives and poly(3-hydroxybutyrate) bioplastic are used as building blocks for the development of both underwater and in-air soft electromechanical actuators that are characterized and tested. A cellulosic matrix is blended with natural-derived ionic liquids to design and manufacture completely biodegradable supercapacitors, extremely interesting energy storage devices. Lastly, ultrathin Au electrodes are here deposited on biodegradable cellulose acetate sheets, in order to develop transparent flexible electronics as well as bidirectional resistive-type strain sensors. The results obtained in this work can be regarded as a preliminary study towards the realization of full natural-derived and biodegradable soft robotic and electronic systems and devices