6,551 research outputs found
A Survey on Wireless Sensor Network Security
Wireless sensor networks (WSNs) have recently attracted a lot of interest in
the research community due their wide range of applications. Due to distributed
nature of these networks and their deployment in remote areas, these networks
are vulnerable to numerous security threats that can adversely affect their
proper functioning. This problem is more critical if the network is deployed
for some mission-critical applications such as in a tactical battlefield.
Random failure of nodes is also very likely in real-life deployment scenarios.
Due to resource constraints in the sensor nodes, traditional security
mechanisms with large overhead of computation and communication are infeasible
in WSNs. Security in sensor networks is, therefore, a particularly challenging
task. This paper discusses the current state of the art in security mechanisms
for WSNs. Various types of attacks are discussed and their countermeasures
presented. A brief discussion on the future direction of research in WSN
security is also included.Comment: 24 pages, 4 figures, 2 table
Bulk de novo mitogenome assembly from pooled total DNA elucidates the phylogeny of weevils (Coleoptera: Curculionoidea)
Complete mitochondrial genomes have been shown to be reliable markers for phylogeny reconstruction among diverse animal groups. However, the relative difficulty and high cost associated with obtaining de novo full mitogenomes have frequently led to conspicuously low taxon sampling in ensuing studies. Here, we report the successful use of an economical and accessible method for assembling complete or near-complete mitogenomes through shot-gun next-generation sequencing of a single library made from pooled total DNA extracts of numerous target species. To avoid the use of separate indexed libraries for each specimen, and an associated increase in cost, we incorporate standard polymerase chain reaction-based âbaitâ sequences to identify the assembled mitogenomes. The method was applied to study the higher level phylogenetic relationships in the weevils (Coleoptera: Curculionoidea), producing 92 newly assembled mitogenomes obtained in a single Illumina MiSeq run. The analysis supported a separate origin of wood-boring behavior by the subfamilies Scolytinae, Platypodinae, and Cossoninae. This finding contradicts morphological hypotheses proposing a close relationship between the first two of these but is congruent with previous molecular studies, reinforcing the utility of mitogenomes in phylogeny reconstruction. Our methodology provides a technically simple procedure for generating densely sampled trees from whole mitogenomes and is widely applicable to groups of animals for which bait sequences are the only required prior genome knowledge
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