Optimization in Differentiable Manifolds in Order to Determine the Method of Construction of Prehistoric Wall-Paintings

In this paper a general methodology is introduced for the determination of potential prototype curves used for the drawing of prehistoric wall-paintings. The approach includes a) preprocessing of the wall-paintings contours to properly partition them, according to their curvature, b) choice of prototype curves families, c) analysis and optimization in 4-manifold for a first estimation of the form of these prototypes, d) clustering of the contour parts and the prototypes, to determine a minimal number of potential guides, e) further optimization in 4-manifold, applied to each cluster separately, in order to determine the exact functional form of the potential guides, together with the corresponding drawn contour parts. The introduced methodology simultaneously deals with two problems: a) the arbitrariness in data-points orientation and b) the determination of one proper form for a prototype curve that optimally fits the corresponding contour data. Arbitrariness in orientation has been dealt with a novel curvature based error, while the proper forms of curve prototypes have been exhaustively determined by embedding curvature deformations of the prototypes into 4-manifolds. Application of this methodology to celebrated wall-paintings excavated at Tyrins, Greece and the Greek island of Thera, manifests it is highly probable that these wall-paintings had been drawn by means of geometric guides that correspond to linear spirals and hyperbolae. These geometric forms fit the drawings' lines with an exceptionally low average error, less than 0.39mm. Hence, the approach suggests the existence of accurate realizations of complicated geometric entities, more than 1000 years before their axiomatic formulation in Classical Ages

Accurate detection of dysmorphic nuclei using dynamic programming and supervised classification

A vast array of pathologies is typified by the presence of nuclei with an abnormal morphology. Dysmorphic nuclear phenotypes feature dramatic size changes or foldings, but also entail much subtler deviations such as nuclear protrusions called blebs. Due to their unpredictable size, shape and intensity, dysmorphic nuclei are often not accurately detected in standard image analysis routines. To enable accurate detection of dysmorphic nuclei in confocal and widefield fluorescence microscopy images, we have developed an automated segmentation algorithm, called Blebbed Nuclei Detector (BleND), which relies on two-pass thresholding for initial nuclear contour detection, and an optimal path finding algorithm, based on dynamic programming, for refining these contours. Using a robust error metric, we show that our method matches manual segmentation in terms of precision and outperforms state-of-the-art nuclear segmentation methods. Its high performance allowed for building and integrating a robust classifier that recognizes dysmorphic nuclei with an accuracy above 95%. The combined segmentation-classification routine is bound to facilitate nucleus-based diagnostics and enable real-time recognition of dysmorphic nuclei in intelligent microscopy workflows