Segmentation in large-scale cellular electron microscopy with deep learning: A literature survey

Electron microscopy (EM) enables high-resolution imaging of tissues and cells based on 2D and 3D imaging techniques. Due to the laborious and time-consuming nature of manual segmentation of large-scale EM datasets, automated segmentation approaches are crucial. This review focuses on the progress of deep learning-based segmentation techniques in large-scale cellular EM throughout the last six years, during which significant progress has been made in both semantic and instance segmentation. A detailed account is given for the key datasets that contributed to the proliferation of deep learning in 2D and 3D EM segmentation. The review covers supervised, unsupervised, and self-supervised learning methods and examines how these algorithms were adapted to the task of segmenting cellular and sub-cellular structures in EM images. The special challenges posed by such images, like heterogeneity and spatial complexity, and the network architectures that overcame some of them are described. Moreover, an overview of the evaluation measures used to benchmark EM datasets in various segmentation tasks is provided. Finally, an outlook of current trends and future prospects of EM segmentation is given, especially with large-scale models and unlabeled images to learn generic features across EM datasets

Analysis of physiological signals using machine learning methods

Technological advances in data collection enable scientists to suggest novel approaches, such as Machine Learning algorithms, to process and make sense of this information. However, during this process of collection, data loss and damage can occur for reasons such as faulty device sensors or miscommunication. In the context of time-series data such as multi-channel bio-signals, there is a possibility of losing a whole channel. In such cases, existing research suggests imputing the missing parts when the majority of data is available. One way of understanding and classifying complex signals is by using deep neural networks. The hyper-parameters of such models have been optimised using the process of back propagation. Over time, improvements have been suggested to enhance this algorithm. However, an essential drawback of the back propagation can be the sensitivity to noisy data. This thesis proposes two novel approaches to address the missing data challenge and back propagation drawbacks: First, suggesting a gradient-free model in order to discover the optimal hyper-parameters of a deep neural network. The complexity of deep networks and high-dimensional optimisation parameters presents challenges to find a suitable network structure and hyper-parameter configuration. This thesis proposes the use of a minimalist swarm optimiser, Dispersive Flies Optimisation(DFO), to enable the selected model to achieve better results in comparison with the traditional back propagation algorithm in certain conditions such as limited number of training samples. The DFO algorithm offers a robust search process for finding and determining the hyper-parameter configurations. Second, imputing whole missing bio-signals within a multi-channel sample. This approach comprises two experiments, namely the two-signal and five-signal imputation models. The first experiment attempts to implement and evaluate the performance of a model mapping bio-signals from A toB and vice versa. Conceptually, this is an extension to transfer learning using CycleGenerative Adversarial Networks (CycleGANs). The second experiment attempts to suggest a mechanism imputing missing signals in instances where multiple data channels are available for each sample. The capability to map to a target signal through multiple source domains achieves a more accurate estimate for the target domain. The results of the experiments performed indicate that in certain circumstances, such as having a limited number of samples, finding the optimal hyper-parameters of a neural network using gradient-free algorithms outperforms traditional gradient-based algorithms, leading to more accurate classification results. In addition, Generative Adversarial Networks could be used to impute the missing data channels in multi-channel bio-signals, and the generated data used for further analysis and classification tasks

Disease diagnosis in smart healthcare: Innovation, technologies and applications

To promote sustainable development, the smart city implies a global vision that merges artificial intelligence, big data, decision making, information and communication technology (ICT), and the internet-of-things (IoT). The ageing issue is an aspect that researchers, companies and government should devote efforts in developing smart healthcare innovative technology and applications. In this paper, the topic of disease diagnosis in smart healthcare is reviewed. Typical emerging optimization algorithms and machine learning algorithms are summarized. Evolutionary optimization, stochastic optimization and combinatorial optimization are covered. Owning to the fact that there are plenty of applications in healthcare, four applications in the field of diseases diagnosis (which also list in the top 10 causes of global death in 2015), namely cardiovascular diseases, diabetes mellitus, Alzheimer’s disease and other forms of dementia, and tuberculosis, are considered. In addition, challenges in the deployment of disease diagnosis in healthcare have been discussed

A Novel Deep Learning, Camera, and Sensorbased System for Enforcing Hand Hygiene Compliance in Healthcare Facilities

Hospital-acquired infections are a major cause of death worldwide, and poor hand hygiene compliance is a primary reason for their spread. This paper proposes an artificial intelligence, microcontroller, and sensor-based system that monitors and improves staff hand hygiene compliance at various critical points in a hospital. The system uses a Convolutional Neural Network (CNN) to detect and track if staff have followed the WHO hand rub/hand wash guidelines at alcohol dispensers, hospital sinks, and patient beds. The system also uses RFID tags, vibration motors, LEDs, and a central server to identify staff, alert them of their cleaning requirements, monitor their cleaning activity, and report compliance data. We obtain an accuracy of 90.6% in classifying all steps of the WHO-stipulated hand wash/hand rub guidelines and a testing accuracy of 89.8% on Ivanovs et al.’s dataset. The system ensures that hospital staff stay compliant to all WHO hand hygiene guidelines, saving countless lives

Advanced Computational Methods for Oncological Image Analysis

[Cancer is the second most common cause of death worldwide and encompasses highly variable clinical and biological scenarios. Some of the current clinical challenges are (i) early diagnosis of the disease and (ii) precision medicine, which allows for treatments targeted to specific clinical cases. The ultimate goal is to optimize the clinical workflow by combining accurate diagnosis with the most suitable therapies. Toward this, large-scale machine learning research can define associations among clinical, imaging, and multi-omics studies, making it possible to provide reliable diagnostic and prognostic biomarkers for precision oncology. Such reliable computer-assisted methods (i.e., artificial intelligence) together with clinicians’ unique knowledge can be used to properly handle typical issues in evaluation/quantification procedures (i.e., operator dependence and time-consuming tasks). These technical advances can significantly improve result repeatability in disease diagnosis and guide toward appropriate cancer care. Indeed, the need to apply machine learning and computational intelligence techniques has steadily increased to effectively perform image processing operations—such as segmentation, co-registration, classification, and dimensionality reduction—and multi-omics data integration.