A hybrid method to characterise the mechanical behaviour of biological hyper-elastic tissues

The characterisation of biological tissues has become high interest area in recent years, due to the grow uses and development of artificial soft tissues implants. These tissues present a nonlinear mechanical behaviour, which highly differs from typical engineering materials. This aspect brings us an enormous difficulty in characterisation of soft tissues and thus required the development of new experimental techniques associated with new numerical algorithms. This work presents the mechanical characterisation of human vaginal mucosa based on a hybrid technique that combines the experimental measurement of displacement field, acquired during a tensile test with numerical simulation, using material constitutive laws. The digital image correlation technique was used for high spatial resolution measurement of the displacements field on the hyper-elastic biological tissues. Several numerical simulations were carryout based on finite element commercial package, Ansys®, by combining the experimental displacements with different hyper-elastic models, which were developed from the experimental tensile test. Fluid release from specimen was observed during the tensile test, producing speckle decorrelation and, therefore, lack of information in displacement field. This problem was overcome by extrapolating data at the boundaries, through the application of special algorithm developed by the authors. The proposed hybrid method is shown to be more acquired then the numerical method based only on material constitutive models

The mechanical design aspects of a small diameter vascular prosthesis

Bibliography: pages 81-86.Failure of medium to small diameter vascular grafts is believed to be in part due to the compliance mismatch between the native artery and the implanted graft. Consequently, designers are examining the use of more compliant materials for their manufacture. Ether free polyurethanes are currently amongst the most popular materials for use in biological implants although these materials are inherently too stiff for use in vascular prostheses. These materials can be made more compliant by introducing porosity. Apart from creating a more compliant overall material, under optimal biological conditions, the porosity may lead to cell in growth through the thickness of the graft allowing an endothelial cell layer to form on the inner flow surface. Compliance and cell ingrowth are both important characteristics that determine the successful functioning of the graft. The current work is part of a collaborative venture with the Cardiovascular Research Unit (CVRU) at the University of Cape Town to design and develop a new polyurethane graft. Finite element models are used to facilitate stress analyses and to evaluate the long-term behaviour and compliance of various graft designs made from a bio-inert thermoplastic polyurethane. Material properties of the polyurethane are determined from uniaxial tension tests, simple-shear tests and viscoelastic shear tests. The constitutive equations for a compressible, large strain hyper elastic material model with viscoelasticity are implemented in the finite element code using material constants calculated from the test data. The behaviour of the finite element model is verified by using a single element test and comparing results to the material data. The finite element model is validated for use m more sophisticated problems by comparing axi-symmetric models with in vitro experiments. An artery/graft anastomosis is then analysed by modelling the artery as an incompressible hyperplastic material. Further more complex graft designs are analysed with internal growth channels and spiral reinforcing winds. Viscoelastic effects are also examined. The modelling method is discussed and important results are noted

Design of human surrogates for the study of biomechanical injury: a review

Human surrogates are representations of living human structures employed to replicate “real-life” injurious scenarios in artificial environments. They are used primarily to evaluate personal protective equipment (PPE) or integrated safety systems (e.g., seat belts) in a wide range of industry sectors (e.g., automotive, military, security service, and sports equipment). Surrogates are commonly considered in five major categories relative to their form and functionality: human volunteers, postmortem human surrogates, animal surrogates, anthropomorphic test devices, and computational models. Each surrogate has its relative merits. Surrogates have been extensively employed in scenarios concerning “life-threatening” impacts (e.g., penetrating bullets or automotive accidents). However, more frequently occurring nonlethal injuries (e.g., fractures, tears, lacerations, contusions) often result in full or partial debilitation in contexts where optimal human performance is crucial (e.g., military, sports). Detailed study of these injuries requires human surrogates with superior biofidelity to those currently available if PPE designs are to improve. The opportunities afforded by new technologies, materials, instrumentation, and processing capabilities should be exploited to develop a new generation of more sophisticated human surrogates. This paper presents a review of the current state of the art in human surrogate construction, highlighting weaknesses and opportunities, to promote research into improved surrogates for PPE development

Modelling mitral valvular dynamics–current trend and future directions

Dysfunction of mitral valve causes morbidity and premature mortality and remains a leading medical problem worldwide. Computational modelling aims to understand the biomechanics of human mitral valve and could lead to the development of new treatment, prevention and diagnosis of mitral valve diseases. Compared with the aortic valve, the mitral valve has been much less studied owing to its highly complex structure and strong interaction with the blood flow and the ventricles. However, the interest in mitral valve modelling is growing, and the sophistication level is increasing with the advanced development of computational technology and imaging tools. This review summarises the state-of-the-art modelling of the mitral valve, including static and dynamics models, models with fluid-structure interaction, and models with the left ventricle interaction. Challenges and future directions are also discussed

The development of a soft tissue mimicking hydrogel: Mechanical characterisation and 3D printing

Accurate tissue phantoms are difficult to design due to the complex hyperelastic, viscoelastic and biphasic properties of real soft tissues. The aim of this work is to demonstrate the tissue mimicking ability of a composite hydrogel (CH), constituting of poly(vinyl alcohol) (PVA) and phytagel (PHY), as a soft tissue phantom over a range mechanical properties, for a variety of biomedical and tissue engineering applications. Its compressive stress-strain behaviour, relaxation response, tensile impact stresses and surgical needle-tissue interactions were mapped and characterised with respect to its constituent hydrogel formulation. The mechanical characterisation of biological tissues was also investigated and the results were used as the ground truth for mimicking. The best mimicking hydrogel compositions were determined by combining the most relevant mechanical properties for each desired application. This thesis demonstrates the use of the tissue mimicking composite hydrogel formulations as tissue phantoms for various surgical procedures, including convection enhanced drug delivery, and traumatic brain injury studies. To expand the applications of the CH, a preliminary biological evaluation of the hydrogel was performed using human dermal fibroblasts. Cell seeded on the collagen-coated composite hydrogel showed good attachment and viability. Finally, a novel fabrication method with the aim of creating samples that replicate the anisotropic properties of biological tissues was developed. A cryogenic 3D printing method utilising the liquid to solid phase change of the composite hydrogel ink was achieved by rapidly cooling the ink solution below its freezing point. The setup was able to successfully create complex 3D brain mimicking material. The method was validated by showing that the mechanical and microstructural properties of the 3D printed material was well matched to its cast-moulded equivalent. This greatly widens the applications of the CH as a mechanically accurate tool for in-vitro testing and also demonstrates promise for future mechanobiology and tissue engineering studies.Open Acces

Investigation of the Dynamic Interaction between the Human Body and Car Seat Using a Unique Simulation Technique

Numerical simulations and mathematical models have been developed over last many years on the certain portions of human body, car seat or automobile to characterise, monitor and assess the level of vibration and its effects on the human occupant inside the automotive. Though, the numerical simulations can define the level and nature of vibration and its transmissibility up to a certain stage, vibration measurement techniques have also been gaining importance for last several years to fill the limitations of the theoretical models. Efforts have also been made to carry out vibration related investigations using combined numerical simulation and measurement procedure for the car seat and the seated human body inside car, though the numbers of case studies carried out with the combination of simulation and measurement procedure are very less. Some technologies have been achieved to judge the level of vibration inside the car seat and its human occupant, though those technologies cover only effects of vibration, dynamics or measurement techniques on specific portions of the car or the human body without considering all the real life factors, e.g., human gender, shape of the human portions, size specific stiffness properties, in-vehicle operating conditions and damping factors. Approaches to provide a comprehensive solution to estimate the level of vibration without real life testing have not been carried out by the existing technologies very well. More than that the existing technologies investigate only particular modules of the entire human-car dynamic systems, e.g., a specific human part, seat and human interaction, vibration transmission from seat to human body or the vibration measurement technique. So, there is enormous scope of further improvement and the aim of this research work is to provide a unique simulated system considering all the critical real life factors. Outcome of this simulation study will evaluate the vibration levels inside the segments of seated human body inside a car and car seat omitting the necessity of real-life practical testing and provide the solution by linking module-wise investigations of human body and car seat. Initiative has been taken to fill up the gaps in the existing technologies and offer a novel study on the comprehensive simulation model of the combined human body and car seat bio-dynamic system to optimize the health, safety and comfort levels of the car seated human body. Present research work covered the tasks of establishing the simulations for non-linear bio-dynamic model of the seated human body, feasibility and behaviour inspection of polyurethane foam cushions, contact mechanism assignment between the human body and the car seat and establishing the simulation of car seated human occupant under the real life environment. Vertical displacements, vertical accelerations and frequencies at designated points of human body and car seat have been extracted from the simulation outcome and the obtained results have been validated though real-life vibration testing data. This unique simulation methodology can successfully be implemented to predict the final vibration levels inside the car seat and the car seated human body to optimize the health, safety and comfort of the human-car seat system. The outlined novel technique contributed knowledge to the entire human body and car seat dynamic system rather than focusing only on a very specific portion of the system. An industrial guideline has been presented to implement this unique simulation methodology in similar sectors, which will lead various industries to avoid time consuming and expensive bio-dynamic vibration testing methods and help to understand the impact of vibration on the in-vehicle human body in a better way

Development of a Fabrication Technique for Soft Planar Inflatable Composites

Soft robotics is a rapidly growing field in robotics that combines aspects of biologically inspired characteristics to unorthodox methods capable of conforming and/or adapting to unknown tasks or environments that would otherwise be improbable or complex with conventional robotic technologies. The field of soft robotics has grown rapidly over the past decade with increasing popularity and relevance to real-world applications. However, the means of fabricating these soft, compliant and intricate robots still poses a fundamental challenge, due to the liberal use of soft materials that are difficult to manipulate in their original state such as elastomers and fabric. These material properties rely on informal design approaches and bespoke fabrication methods to build soft systems. As such, there are a limited variety of fabrication techniques used to develop soft robots which hinders the scalability of robots and the time to manufacture, thus limiting their development. This research focuses towards developing a novel fabrication method for constructing soft planar inflatable composites. The fundamental method is based on a sub-set of additive manufacturing known as composite layering. The approach is designed from a planar manner and takes layers of elastomeric materials, embedded strain-limiting and mask layers. These components are then built up through a layer-by-layer fabrication method with the use of a bespoke film applicator set-up. This enables the fabrication of millimetre-scale soft inflatable composites with complex integrated masks and/or strain-limiting layers. These inflatable composites can then be cut into a desired shape via laser cutting or ablation. A design approach was also developed to expand the functionality of these inflatable composites through modelling and simulation via finite element analysis. Proof of concept prototypes were designed and fabricated to enable pneumatic driven actuation in the form of bending soft actuators, adjustable stiffness sensor, and planar shape change. This technique highlights the feasibility of the fabrication method and the value of its use in creating multi-material composite soft actuators which are thin, compact, flexible, and stretchable and can be applicable towards real-world application

Assessment of head injury risk caused by impact using finite element models

[ES] Las cargas de impacto son la fuente primaria de lesiones en la cabeza y pueden resultar en un rango de traumatismo desde leve hasta severo. Debido a la existencia de múltiples entornos en los que se pueden desencadenar lesiones por impacto (accidentes automovilísticos, deportes, caídas accidentales, violencia), éstas pueden afectar potencialmente a toda la población independientemente de su estado de salud. Pese al creciente esfuerzo en investigación para comprender la biomecánica de las lesiones por traumatismo en la cabeza, todavía no es del todo posible realizar predicciones precisas ni prevenir estos eventos. En esta Tesis, se han estudiado algunos aspectos del comportamiento ante impacto de los diferentes tejidos biológicos involucrados mediante el desarrollo de un modelo numérico de cabeza humana a partir de imágenes de tomografía computerizada (TAC). Se han realizado simulaciones en elementos finitos (EF) de ensayos experimentales de la literatura con el fin de validar el modelo numérico desarrollado, estableciendo unas propiedades mecánicas adecuadas para cada uno de sus constituyentes. De esta manera se puede adquirir una predicción adecuada del riesgo de sufrir daños. Parte de esta Tesis se centra en el entorno balístico, específicamente en cascos de combate antibalas, los cuales son susceptibles de causar traumatismo craneoencefálico debido a la elevada deformación que sufren durante el impacto. Previamente al estudio de estos fenómenos de alta velocidad, se han realizado ensayos experimentales y numéricos para caracterizar la respuesta mecánica de algunos materiales compuestos ante impacto de baja velocidad. Al principio de esta Tesis se ha realizado una revisión del estado del arte acerca de los criterios existentes para cuantificar el trauma craneoencefálico.Este es un aspecto clave para las simulaciones numéricas, ya que la idoneidad de algunos de estos criterios para la predicción de lesiones cerebrales todavía es un debate abierto. Mediante EF se han realizado simulaciones de impactos balísticos en una cabeza protegida con un casco de combate. Mediante la posterior aplicación de diferentes criterios de daño sobre los resultados obtenidos se ha evaluado el nivel de protección que aseguran los protocolos de aceptación de cascos de combate, así como las estrategias para determinar su tallaje. Se ha demostrado que las normativas existentes para cascos de combate son capaces de mitigar algunos mecanismos de trauma pero no logran prevenir otros como los gradientes de presión intracraneales. Además, se ha demostrado que algunas de las estrategias de tallaje más comúnmente adoptadas por los fabricantes, como producir un solo tamaño de calota, deberían ser reconsideradas ya que existe un mayor riesgo de traumatismo cuando la distancia entre la cabeza y la calota del casco no es suficiente. Siguiendo la línea de protecciones personales, algunos de los materiales compuestos comúnmente empleados en la industria armamentística se han combinado para crear distintas configuraciones de calota para optimizar la relación entre peso del casco y protección para la cabeza. Materiales ligeros como el UHMWPE han resultado en un comportamiento menos eficiente que el de los apilados de tejido de aramida a la hora de limitar la BFD (deformación máxima en la calota del casco en la zona de impacto). Hacia el final de la Tesis se presenta un modelo numérico de cabeza humana detallado, que incluye treinta y tres de las estructuras anatómicas principales. Dicho modelo se ha desarrollado para la simulación de un accidente ecuestre en el que aparecen múltiples lesiones craneoencefálicas. Principalmente, se pretende establecer un criterio mecánico para predecir el hematoma subdural (HS) basado en la ruptura de los vasos sanguíneos intracraneales. Se ha propuesto un valor umbral de ruptura en tensiones de 3.5 MPa, pero tanto este límite como la localización del vaso dañado son altamen[CA] Les càrregues d'impacte son la font primària de lesions al cap i poden resultar en un rang de severitat des de lleu a greu. Degut als múltiples entorns en que poden desencadenar-se lesions per impacte (accidents automobilístics, esports, caigudes accidentals, violència), aquestes poden afectar potencialment a tota la població independentment del seu estat de salut. Malgrat el creixent esforç en investigació per comprendre la biomecànica de les lesions per traumatisme al cap, encara no és del tot possible realitzar prediccions precises ni prevenir aquestos esdeveniments. En aquesta Tesi, s'han estudiat alguns aspectes del comportament a impacte dels diferents teixits biològics involucrats mitjançant el desenvolupament d'un model numèric de cap humà a partir d'imatges de tomografia computeritzada (TAC). S'han realitzat simulacions en elements finits (EF) d'assajos experimentals de la literatura amb la finalitat de validar el model numèric desenvolupat, establint unes propietats mecàniques adequades per a cadascun dels seus constituents. D'aquesta manera es pot aconseguir una predicció del risc de sofrir danys traumàtics. Part d'aquesta Tesi es centra en l'entorn balístic, específicament en cascs de combat antibales, els quals són susceptibles de causar traumatisme degut a l'elevada deformació que sofrixen durant l'impacte. Previament a l'estudi d'aquests fenòmens d'alta velocitat, s'han realitzat assajos experimentals i numèrics per a caracteritzar la resposta mecànica d'alguns materials compostos en condicions d'impacte a baixa velocitat. Al començament d'aquesta Tesi s'ha realitzat una revisió de l'estat de l'art sobre els criteris existents per quantificar el trauma cranioencefàlic. Aquest és un aspecte clau per a les simulacions numèriques, ja que l'utilitat d'alguns d'aquestos criteris per a la predicció de lesions cerebrals és encara un debat obert. Mitjançant EF s'han realitzat simulacions numèriques d'impactes balístics en un cap protegit amb un casc de combat. Gràcies a la posterior aplicació de diferents criteris de dany sobre els resultats obtinguts s'ha evaluat el nivell de protecció que asseguren els protocols d'acceptació de cascs de combat, així com les estratègies per a determinar les seues talles. S'ha demostrat que les normatives existents són capaces de mitigar alguns mecanismes de trauma però no aconseguixen prevenir altres com els gradients de pressions intracranials. A més, s'ha demostrat que algunes estratègies per determinar les talles més comunament adoptades pels fabricants (com produir només un tamany de calota i adaptar el gruix de les escumes interiors a les diferents dimensions dels subjectes) haurien de ser reconsiderades ja que existeix un major risc de traumatisme quan la distància entre el cap i la calota del casc no és suficient. Seguint la línia de proteccions personals, alguns dels materials compostos comunament utilitzats en la indústria de l'armament s'han combinat per a crear distintes possibles configuracions de calota amb la finalitat d'optimitzar la relació entre pes i protecció. Materials lleugers com l'UHMWPE han resultat en un comportament menys eficient que el d'apilats de teixit d'aramida a l'hora de limitar la BFD (deformació màxima a la calota del casc a la zona d'impacte). Cap al final de la Tesi es presenta un model numèric detallat de cap humà, que inclou trenta-tres de les estructures anatòmiques principals. Aquest model s'ha desenvolupat per a la simulació d'un accident eqüestre en el qual apareixen múltiples lesions cranioencefàliques. Principalment, es pretén establir un criteri mecànic per a la predicció de l'hematoma subdural (HS) basat en la ruptura dels vasos sanguinis intracranials. S'ha proposat un valor umbral de ruptura en tensions de 3.5 MPa, pero tant aquest límit com la ubicació del vas danyat són altament dependents de l'anatomia específica de cada subjecte.[EN] Impact loading is the primary source of head injuries and can result in a range of trauma from mild to severe. Because of the multiple environments in which impact-related injuries can take place (automotive accidents, sports, accidental falls, violence), they can potentially affect the entire population regardless of their health conditions. Despite the increasing research effort on the understanding of head impact biomechanics, accurate prediction and prevention of traumatic injuries has not been completely achieved. In this Thesis, some aspects of the impact behaviour of the different biological tissues involved have been analysed through the development of a numerical human head model from Computed Tomography (CT) images. FE simulations of experimental tests from the literature have been performed and enhanced the validation of the head model through the establishment of proper material laws for its constituents, which enable adequate prediction of injury risks. Part of this Thesis focuses on the ballistic environment, especifically in bulletproof composite helmets, which are susceptible to cause blunt injuries to the head because of their large deformation during impact. Prior to the study of these high-speed impacts, experimental tests and finite element (FE) models have been performed to characterise the mechanical response of composite materials subjected to low velocity impact. The implementation of a continuum damage mechanics approach coupled to a Hashin failure criterion and surface-to-surface cohesive relations to the numerical model provided a good matching with the impact behaviour obtained experimentally, capturing the principal damage mechanisms. A review of the head injury criteria currently available in the literature has been performed at the beginning of this Thesis. This is a key issue for the numerical simulations, as the suitability of some criteria to predict head injuries is still an open question. Numerical simulation of ballistic impacts on a human head protected with a combat helmet has been conducted employing explicit FE analysis. The level of protection ensured by helmet acceptance protocols as well as their sizing strategies have been studied and discussed by means of the application of different mechanical-based head injury criteria. It has been demonstrated that current helmet testing standards do mitigate some specific forms of head trauma but fail to prevent other injury mechanisms such as the intracranial pressure gradients within the skull. Furthermore, it has been demonstrated that some well-established helmet sizing policies like manufacturing one single composite shell and adapting the thickness of the interior pads to the different head dimensions should be reconsidered, as there is a great risk of head injury when the distance between the head and the helmet shell (stand-off distance) is not sufficient. Following the line of personal protections, some composite materials commonly employed in the soft body armour industry have been combined into different helmet shells configurations to optimise the ratio of weight-to-head protection. Light materials like UHMWPE appear to be less efficient than integral woven-aramid lay-ups in the limitation of the backface deformation (BFD), the maximum deformation sustained by the helmet at the impact site. A detailed head numerical model including thirty-three of its main anatomical structures has been developed for the simulation of an equestrian accident that resulted in many head injuries. Above all, the establishment of a mechanical criterion for the prediction of subdural hematona (SDH) based on the rupture of the head blood vessels is intended. A stress threshold for vein rupture has been set on 3.5 MPa, but both this limit and the location of vessel failure are highly dependent on the specific anatomy of the subject's vascularity.Palomar Toledano, M. (2019). Assessment of head injury risk caused by impact using finite element models [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/135254TESI