Light-sheet microscopy: a tutorial

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.Peer ReviewedPostprint (published version

Dynamic Object Tracking for Quadruped Manipulator with Spherical Image-Based Approach

Exactly estimating and tracking the motion of surrounding dynamic objects is one of important tasks for the autonomy of a quadruped manipulator. However, with only an onboard RGB camera, it is still a challenging work for a quadruped manipulator to track the motion of a dynamic object moving with unknown and changing velocities. To address this problem, this manuscript proposes a novel image-based visual servoing (IBVS) approach consisting of three elements: a spherical projection model, a robust super-twisting observer, and a model predictive controller (MPC). The spherical projection model decouples the visual error of the dynamic target into linear and angular ones. Then, with the presence of the visual error, the robustness of the observer is exploited to estimate the unknown and changing velocities of the dynamic target without depth estimation. Finally, the estimated velocity is fed into the model predictive controller (MPC) to generate joint torques for the quadruped manipulator to track the motion of the dynamical target. The proposed approach is validated through hardware experiments and the experimental results illustrate the approach's effectiveness in improving the autonomy of the quadruped manipulator

Design and Demonstration of a Two-Dimentional Test Bed for UAV Controller Evaluation

A three degree-of-freedom (DOF) planar test bed for Unmanned Aerial Vehicle (UAV) controller evaluation was built. The test-bed consists of an instrumented tether and an experimental twin-rotor, planar UAV mounted with a one DOF manipulator mounted below the UAV body. The tether was constructed to constrain the UAV under test to motion on the surface of a sphere. Experiments can be conducted through the tether, approximating motion in a vertical plane by a UAV under test. The tether provides the means to measure the position and attitude of the UAV under test. The experimental twin-rotor UAV and one-link on-board manipulator, were designed and built to explore a unified control strategy for Manipulator on VTOL Aircraft (MOVA), in which the interaction of UAV body dynamics with the manipulator motion is of primary interest. The dynamics of the propulsion unit was characterized through experiments, based on which a phase lead compensator was designed to improve the UAV frequency response. A \u27separate\u27 controller based on independent nonlinear control of the VTOL aircraft and PD linear control of the on-board manipulator was designed as a reference for comparison to the unified MOVA controller. Tests with the separate controller show the negative effect that a coupled manipulator can have on the UAV body motion, while the tests on MOVA show the potential benefit of explicit compensation of the UAV and manipulator interaction

Design and Development of a Surgical Robot for Needle-Based Medical Interventions

Lung cancer is the leading cause of cancer related deaths. If diagnosed in a timely manner, the treatment of choice is surgical resection of the cancerous lesions followed by radiotherapy. However, surgical resection may be too invasive for some patients due to old age or weakness. An alternative is minimally invasive needle-based interventions for cancer diagnosis and treatment. This project describes the design, analysis, development and experimental evaluation of a modular, compact, patient-mounted robotic manipulator for lung cancer diagnosis and treatment. In this regard, a novel parallel Remote Centre of Motion (RCM) mechanism is proposed for minimally invasive delivery of needle-based interventions. The proposed robot provides four degrees of freedom (DOFs) to orient and move a surgical needle within a spherical coordinate system. There is an analytical solution for the kinematics of the proposed parallel mechanism and the end-effectors motion is well-conditioned within the required workspace. The RCM is located beneath the skin surface to minimize the invasiveness of the surgical procedure while providing the required workspace to target the cancerous lesions. In addition, the proposed robot benefits from a design capable of measuring the interaction forces between the needle and the tissue. The experimental evaluation of the robot has proved its capability to accurately orient and move a surgical needle within the required workspace. Although this robotic system has been designed for the treatment of lung cancer, it is capable of performing other procedures in the thoracic or abdominal cavity such as liver cancer diagnosis and treatment

RomniStereo: Recurrent Omnidirectional Stereo Matching

Omnidirectional stereo matching (OSM) is an essential and reliable means for $360^{\circ}$ depth sensing. However, following earlier works on conventional stereo matching, prior state-of-the-art (SOTA) methods rely on a 3D encoder-decoder block to regularize the cost volume, causing the whole system complicated and sub-optimal results. Recently, the Recurrent All-pairs Field Transforms (RAFT) based approach employs the recurrent update in 2D and has efficiently improved image-matching tasks, ie, optical flow, and stereo matching. To bridge the gap between OSM and RAFT, we mainly propose an opposite adaptive weighting scheme to seamlessly transform the outputs of spherical sweeping of OSM into the required inputs for the recurrent update, thus creating a recurrent omnidirectional stereo matching (RomniStereo) algorithm. Furthermore, we introduce two techniques, ie, grid embedding and adaptive context feature generation, which also contribute to RomniStereo's performance. Our best model improves the average MAE metric by 40.7\% over the previous SOTA baseline across five datasets. When visualizing the results, our models demonstrate clear advantages on both synthetic and realistic examples. The code is available at \url{https://github.com/HalleyJiang/RomniStereo}.Comment: accepted by IEEE RA-L, https://github.com/HalleyJiang/RomniStere

Intraoperative Endoscopic Augmented Reality in Third Ventriculostomy

In neurosurgery, as a result of the brain-shift, the preoperative patient models used as a intraoperative reference change. A meaningful use of the preoperative virtual models during the operation requires for a model update. The NEAR project, Neuroendoscopy towards Augmented Reality, describes a new camera calibration model for high distorted lenses and introduces the concept of active endoscopes endowed with with navigation, camera calibration, augmented reality and triangulation modules