An end-to-end software solution for the analysis of high-throughput single-cell migration data

The systematic study of single-cell migration requires the availability of software for assisting data inspection, quality control and analysis. This is especially important for high-throughput experiments, where multiple biological conditions are tested in parallel. Although the field of cell migration can count on different computational tools for cell segmentation and tracking, downstream data visualization, parameter extraction and statistical analysis are still left to the user and are currently not possible within a single tool. This article presents a completely new module for the open-source, cross-platform CellMissy software for cell migration data management. This module is the first tool to focus specifically on single-cell migration data downstream of image processing. It allows fast comparison across all tested conditions, providing automated data visualization, assisted data filtering and quality control, extraction of various commonly used cell migration parameters, and non-parametric statistical analysis. Importantly, the module enables parameters computation both at the trajectory-and at the step-level. Moreover, this single-cell analysis module is complemented by a new data import module that accommodates multiwell plate data obtained from high-throughput experiments, and is easily extensible through a plugin architecture. In conclusion, the end-to-end software solution presented here tackles a key bioinformatics challenge in the cell migration field, assisting researchers in their highthroughput data processing

Digital Preservation Services : State of the Art Analysis

Research report funded by the DC-NET project.An overview of the state of the art in service provision for digital preservation and curation. Its focus is on the areas where bridging the gaps is needed between e-Infrastructures and efficient and forward-looking digital preservation services. Based on a desktop study and a rapid analysis of some 190 currently available tools and services for digital preservation, the deliverable provides a high-level view on the range of instruments currently on offer to support various functions within a preservation system.European Commission, FP7peer-reviewe

Process migration in UNIX environments

To support process migration in UNIX environments, the main problem is how to encapsulate the location dependent features of the system in such a way that a host independent virtual environment is maintained by the migration handlers on the behalf of each migrated process. An object-oriented approach is used to describe the interaction between a process and its environment. More specifically, environmental objects were introduced in UNIX systems to carry out the user-environment interaction. The implementation of the migration handlers is based on both the state consistency criterion and the property consistency criterion

Key Success Factors for the Project of Migrating to the Open Office Suite

The penetration and performance of free software is raising issues regarding its true capacities and, particularly, the desirability of choosing it. It is from this perspective that the Linux Migration Project was launched within the Sous-secrétariat à l’inforoute gouvernementale et aux resources informationnelles (SSIGRI). Its accompaniment by a team of researchers from CIRANO is intended to assess the risks and identify the conditions for success. The purpose of this report is to identify and assess the key success factors of this project. Principal results An analysis of the project’s characteristics has enabled its specific features to be identified and the analytical tool to be adapted. From this approach, analysis of the key success factors has revealed that the pilot project substantially contributes to the reflection about migrating to free software. It demonstrates that, despite medium to high risk exposure, such a migration can be controlled. This is supported by considerable managerial ability and the reliability of the technology. Finally, it draws attention to a major problem that arises in a migration context: the absence of a shared interoperability framework, as is seen in two out of three parameters. The assessment grid of the project’s key success factors (Table 1, p. 6) allows the following to be ascertained: The importance of the Risk Assessment and Monitoring factor during the software implementation process. Its estimated value of 3.7, in particular due to the absence of a common interoperability framework and the impossibility of remedying it within the context of the project, lowers the average of the Processes success factor, which is 4.8/7. Managerial skills are high (6.2/7), and the values found for this factor’s components are generally comparable. Technology is assessed at 5.5/7; this parameter covers a contrasted reality: The technology’s intrinsic characteristics (independence with regard to software and publishers, cost controls, data continuity), assessed at 6.6/7, raise this ratio. The technology’s performance, assessed at 4.5/7, lowers this ratio. It implicates both the intrinsically high quality of the software tested, and problems due to the context of the pilot project—characterized, as it was, by the absence of a migration plan (choice of services/people to migrate) and to the absence of a common interoperability framework.