4,820 research outputs found

    The future of cosmology? A case for CMB spectral distortions

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    This thesis treats the topic of CMB Spectral Distortions (SDs), which represent any deviation from a pure black body shape of the CMB energy spectrum. As such, they can be used to probe the inflationary, expansion and thermal evolution of the universe both within Λ\LambdaCDM and beyond it. The currently missing observation of this rich probe of the universe makes of it an ideal target for future observational campaigns. In fact, while the Λ\LambdaCDM signal guarantees a discovery, the sensitivity to a wide variety of new physics opens the door to an enormous uncharted territory. In light of these considerations, the thesis opens by reviewing the topic of CMB SDs in a pedagogical and illustrative fashion, aimed at waking the interest of the broader community. This introductory premise sets the stage for the first main contribution of the thesis to the field of SDs: their implementation in the Boltzmann solver CLASS and the parameter inference code MontePython. The CLASS+MontePython pipeline is publicly available, fast, it includes all sources of SDs within Λ\LambdaCDM and many others beyond that, and allows to consistently account for any observational setup. By means of these numerical tools, the second main contribution of the thesis consists in showcasing the versatility and competitiveness of SDs for several cosmological models as well as for a number of different mission designs. Among others, the results cover features in the primordial power spectrum, primordial gravitational waves, non-standard dark matter properties, primordial black holes, primordial magnetic fields and Hubble tension. Finally, the manuscript is disseminated with (20) follow-up ideas that naturally extend the work carried out so far, highlighting how rich of unexplored possibilities the field of CMB SDs still is. The hope is that these suggestions will become a propeller for further interesting developments.Comment: PhD thesis. Pedagogical review of theory, experimental status and numerical tools (CLASS+MontePython) with broad overview of applications. Includes 20 original follow-up idea

    Thermophoresis of electrolyte solutions and protein-ligand systems

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    Thermophoresis or thermodiffusion is the mass transport driven by a temperature gradient. This thesis focuses on the thermophoretic motion of ionic compounds in a biological context and is motivated by a practical application, in which thermodiffusion is used to monitor protein-ligand reactions. Proteins are complex molecules containing non-ionic and ionic groups. While recent studies of non-ionic compounds found a strong correlation between thermodiffusion and hydration, it is unclear how this correlation changes when molecules are charged. To separate ionic from non-ionic contributions, it is reasonable to look first into the thermophoretic motion of simple salts without large organic side groups and to study in the next step complex protein-ligand systems, which typically contain hydrophobic and hydrophilic groups. The systematic studies of aqueous solutions of simple salts should reveal differences between ionic and non-ionic systems and should give further information about ion and ion specific effects. Due to the high complexity of protein-ligand systems, complementary methods should be used to gain a better understanding of the interactions between different components that are present in the system. This will help to understand how the thermophoretic behavior of the free protein differs from that of the protein-ligand complex formed. Study of the thermophoretic behavior of ionic systems indicates that several correlations, which were found for aqueous solutions of non-ionic solutes are no longer valid for ionic solutes. For non-ionic solutes hydrogen bonds primarily influence the thermophoretic behavior. In case of ionic solutes, although both electrostatic interactions and hydrogen bonds are present, it is found that thermophoretic behavior is influenced by electrostatic interactions. Focusing on the specific ion effects for ionic systems in the context of the Hofmeister series, a change of the anion is found to influence the thermophoretic behavior more than a change of the cation. Further, a correlation between thermophoretic behavior and hydrophilicity of the ionic solutes is found, which underlines the sensitivity of thermodiffusion to changes in hydration. Based on this sensitivity, a preliminary model is developed for describing the non-monotonous variation of Soret coefficient ST with concentration for aqueous solutions of alkali iodide salts. To study the thermodiffusion of binding reactions, we also use complementary methods such as Isothermal Titration Calorimetry (ITC) and a thermophoretic microfluidic cell. As systems, we have chosen EDTA-CaCl2 and protein-ligand systems (binding of Bovine Carbonic Anhydrase I (BCA I) with two aryl sulfonamide ligands). To gain deeper insight into the complex formation reactions thermophoretic data (non-equilibrium process) are compared with thermodynamic data (equilibrium process) to establish a mathematical relation between ST and Gibb’s free energy ΔG. For EDTA-CaCl2 and protein-ligand systems, the derived relation holds valid, which enables calculation of ΔG at a particular temperature from ST

    Financial and Economic Review 22.

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    Antimicrobial Peptides Aka Host Defense Peptides – From Basic Research to Therapy

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    This Special Issue reprint will address the most current and innovative developments in the field of HDP research across a range of topics, such as structure and function analysis, modes of action, anti-microbial effects, cell and animal model systems, the discovery of novel host-defense peptides, and drug development

    Intelligent computing : the latest advances, challenges and future

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    Computing is a critical driving force in the development of human civilization. In recent years, we have witnessed the emergence of intelligent computing, a new computing paradigm that is reshaping traditional computing and promoting digital revolution in the era of big data, artificial intelligence and internet-of-things with new computing theories, architectures, methods, systems, and applications. Intelligent computing has greatly broadened the scope of computing, extending it from traditional computing on data to increasingly diverse computing paradigms such as perceptual intelligence, cognitive intelligence, autonomous intelligence, and human computer fusion intelligence. Intelligence and computing have undergone paths of different evolution and development for a long time but have become increasingly intertwined in recent years: intelligent computing is not only intelligence-oriented but also intelligence-driven. Such cross-fertilization has prompted the emergence and rapid advancement of intelligent computing

    Pollution-induced community tolerance in freshwater biofilms – from molecular mechanisms to loss of community functions

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    Exposure to herbicides poses a threat to aquatic biofilms by affecting their community structure, physiology and function. These changes render biofilms to become more tolerant, but on the downside community tolerance has ecologic costs. A concept that addresses induced community tolerance to a pollutant (PICT) was introduced by Blanck and Wängberg (1988). The basic principle of the concept is that microbial communities undergo pollution-induced succession when exposed to a pollutant over a long period of time, which changes communities structurally and functionally and enhancing tolerance to the pollutant exposure. However, the mechanisms of tolerance and the ecologic consequences were hardly studied up to date. This thesis addresses the structural and functional changes in biofilm communities and applies modern molecular methods to unravel molecular tolerance mechanisms. Two different freshwater biofilm communities were cultivated for a period of five weeks, with one of the communities being contaminated with 4 μg L-1 diuron. Subsequently, the communities were characterized for structural and functional differences, especially focusing on their crucial role of photosynthesis. The community structure of the autotrophs was assessed using HPLC-based pigment analysis and their functional alterations were investigated using Imaging-PAM fluorometry to study photosynthesis and community oxygen profiling to determine net primary production. Then, the molecular fingerprints of the communities were measured with meta-transcriptomics (RNA-Seq) and GC-based community metabolomics approaches and analyzed with respect to changes in their molecular functions. The communities were acute exposed to diuron for one hour in a dose-response design, to reveal a potential PICT and uncover related adaptation to diuron exposure. The combination of apical and molecular methods in a dose-response design enabled the linkage of functional effects of diuron exposure and underlying molecular mechanisms based on a sensitivity analysis. Chronic exposure to diuron impaired freshwater biofilms in their biomass accrual. The contaminated communities particularly lost autotrophic biomass, reflected by the decrease in specific chlorophyll a content. This loss was associated with a change in the molecular fingerprint of the communities, which substantiates structural and physiological changes. The decline in autotrophic biomass could be due to a primary loss of sensitive autotrophic organisms caused by the selection of better adapted species in the course of chronic exposure. Related to this hypothesis, an increase in diuron tolerance has been detected in the contaminated communities and molecular mechanisms facilitating tolerance have been found. It was shown that genes of the photosystem, reductive-pentose phosphate cycle and arginine metabolism were differentially expressed among the communities and that an increased amount of potential antioxidant degradation products was found in the contaminated communities. This led to the hypothesis that contaminated communities may have adapted to oxidative stress, making them less sensitive to diuron exposure. Moreover, the photosynthetic light harvesting complex was altered and the photoprotective xanthophyll cycle was increased in the contaminated communities. Despite these adaptation strategies, the loss of autotrophic biomass has been shown to impair primary production. This impairment persisted even under repeated short-term exposure, so that the tolerance mechanisms cannot safeguard primary production as a key function in aquatic systems.:1. The effect of chemicals on organisms and their functions .............................. 1 1.1 Welcome to the anthropocene .......................................................................... 1 1.2 From cellular stress responses to ecosystem resilience ................................... 3 1.2.1 The individual pursuit for homeostasis ....................................................... 3 1.2.2 Stability from diversity ................................................................................. 5 1.3 Community ecotoxicology - a step forward in monitoring the effects of chemical pollution? ................................................................................................................. 6 1.4 Functional ecotoxicological assessment of microbial communities ................... 9 1.5 Molecular tools – the key to a mechanistic understanding of stressor effects from a functional perspective in microbial communities? ...................................... 12 2. Aims and Hypothesis ......................................................................................... 14 2.1 Research question .......................................................................................... 14 2.2 Hypothesis and outline .................................................................................... 15 2.3 Experimental approach & concept .................................................................. 16 2.3.1 Aquatic freshwater biofilms as model community ..................................... 16 2.3.2 Diuron as model herbicide ........................................................................ 17 2.3.3 Experimental design ................................................................................. 18 3. Structural and physiological changes in microbial communities after chronic exposure - PICT and altered functional capacity ................................................. 21 3.1 Introduction ..................................................................................................... 21 3.2 Methods .......................................................................................................... 23 3.2.1 Biofilm cultivation ...................................................................................... 23 3.2.2 Dry weight and autotrophic index ............................................................. 23 3.2.4 Pigment analysis of periphyton ................................................................. 23 3.2.4.1 In-vivo pigment analysis for community characterization ....................... 24 3.2.4.2 In-vivo pigment analysis based on Imaging-PAM fluorometry ............... 24 3.2.4.3 In-vivo pigment fluorescence for tolerance detection ............................. 26 3.2.4.4 Ex-vivo pigment analysis by high-pressure liquid-chromatography ....... 27 3.2.5 Community oxygen metabolism measurements ....................................... 28 3.3 Results and discussion ................................................................................... 29 3.3.1 Comparison of the structural community parameters ............................... 29 3.3.2 Photosynthetic activity and primary production of the communities after selection phase ................................................................................................. 33 3.3.3 Acquisition of photosynthetic tolerance .................................................... 34 3.3.4 Primary production at exposure conditions ............................................... 36 3.3.5 Tolerance detection in primary production ................................................ 37 3.4 Summary and Conclusion ........................................................................... 40 4. Community gene expression analysis by meta-transcriptomics ................... 41 4.1 Introduction to meta-transcriptomics ............................................................... 41 4.2. Methods ......................................................................................................... 43 4.2.1 Sampling and RNA extraction................................................................... 43 4.2.2 RNA sequencing analysis ......................................................................... 44 4.2.3 Data assembly and processing................................................................. 45 4.2.4 Prioritization of contigs and annotation ..................................................... 47 4.2.5 Sensitivity analysis of biological processes .............................................. 48 4.3 Results and discussion ................................................................................... 48 4.3.1 Characterization of the meta-transcriptomic fingerprints .......................... 49 4.3.2 Insights into community stress response mechanisms using trend analysis (DRomic’s) ......................................................................................................... 51 4.3.3 Response pattern in the isoform PS genes .............................................. 63 4.5 Summary and conclusion ................................................................................ 65 5. Community metabolome analysis ..................................................................... 66 5.1 Introduction to community metabolomics ........................................................ 66 5.2 Methods .......................................................................................................... 68 5.2.1 Sampling, metabolite extraction and derivatisation................................... 68 5.2.2 GC-TOF-MS analysis ............................................................................... 69 5.2.3 Data processing and statistical analysis ................................................... 69 5.3 Results and discussion ................................................................................... 70 5.3.1 Characterization of the metabolic fingerprints .......................................... 70 5.3.2 Difference in the metabolic fingerprints .................................................... 71 5.3.3 Differential metabolic responses of the communities to short-term exposure of diuron ............................................................................................................ 73 5.4 Summary and conclusion ................................................................................ 78 6. Synthesis ............................................................................................................. 79 6.1 Approaches and challenges for linking molecular data to functional measurements ...................................................................................................... 79 6.2 Methods .......................................................................................................... 83 6.2.1 Summary on the data ............................................................................... 83 6.2.2 Aggregation of molecular data to index values (TELI and MELI) .............. 83 6.2.3 Functional annotation of contigs and metabolites using KEGG ................ 83 6.3 Results and discussion ................................................................................... 85 6.3.1 Results of aggregation techniques ........................................................... 85 6.3.2 Sensitivity analysis of the different molecular approaches and endpoints 86 6.3.3 Mechanistic view of the molecular stress responses based on KEGG functions ............................................................................................................ 89 6.4 Consolidation of the results – holistic interpretation and discussion ............... 93 6.4.1 Adaptation to chronic diuron exposure - from molecular changes to community effects.............................................................................................. 93 6.4.2 Assessment of the ecological costs of Pollution-induced community tolerance based on primary production ............................................................. 94 6.5 Outlook ............................................................................................................ 9

    Examples of works to practice staccato technique in clarinet instrument

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    Klarnetin staccato tekniğini güçlendirme aşamaları eser çalışmalarıyla uygulanmıştır. Staccato geçişlerini hızlandıracak ritim ve nüans çalışmalarına yer verilmiştir. Çalışmanın en önemli amacı sadece staccato çalışması değil parmak-dilin eş zamanlı uyumunun hassasiyeti üzerinde de durulmasıdır. Staccato çalışmalarını daha verimli hale getirmek için eser çalışmasının içinde etüt çalışmasına da yer verilmiştir. Çalışmaların üzerinde titizlikle durulması staccato çalışmasının ilham verici etkisi ile müzikal kimliğe yeni bir boyut kazandırmıştır. Sekiz özgün eser çalışmasının her aşaması anlatılmıştır. Her aşamanın bir sonraki performans ve tekniği güçlendirmesi esas alınmıştır. Bu çalışmada staccato tekniğinin hangi alanlarda kullanıldığı, nasıl sonuçlar elde edildiği bilgisine yer verilmiştir. Notaların parmak ve dil uyumu ile nasıl şekilleneceği ve nasıl bir çalışma disiplini içinde gerçekleşeceği planlanmıştır. Kamış-nota-diyafram-parmak-dil-nüans ve disiplin kavramlarının staccato tekniğinde ayrılmaz bir bütün olduğu saptanmıştır. Araştırmada literatür taraması yapılarak staccato ile ilgili çalışmalar taranmıştır. Tarama sonucunda klarnet tekniğin de kullanılan staccato eser çalışmasının az olduğu tespit edilmiştir. Metot taramasında da etüt çalışmasının daha çok olduğu saptanmıştır. Böylelikle klarnetin staccato tekniğini hızlandırma ve güçlendirme çalışmaları sunulmuştur. Staccato etüt çalışmaları yapılırken, araya eser çalışmasının girmesi beyni rahatlattığı ve istekliliği daha arttırdığı gözlemlenmiştir. Staccato çalışmasını yaparken doğru bir kamış seçimi üzerinde de durulmuştur. Staccato tekniğini doğru çalışmak için doğru bir kamışın dil hızını arttırdığı saptanmıştır. Doğru bir kamış seçimi kamıştan rahat ses çıkmasına bağlıdır. Kamış, dil atma gücünü vermiyorsa daha doğru bir kamış seçiminin yapılması gerekliliği vurgulanmıştır. Staccato çalışmalarında baştan sona bir eseri yorumlamak zor olabilir. Bu açıdan çalışma, verilen müzikal nüanslara uymanın, dil atış performansını rahatlattığını ortaya koymuştur. Gelecek nesillere edinilen bilgi ve birikimlerin aktarılması ve geliştirici olması teşvik edilmiştir. Çıkacak eserlerin nasıl çözüleceği, staccato tekniğinin nasıl üstesinden gelinebileceği anlatılmıştır. Staccato tekniğinin daha kısa sürede çözüme kavuşturulması amaç edinilmiştir. Parmakların yerlerini öğrettiğimiz kadar belleğimize de çalışmaların kaydedilmesi önemlidir. Gösterilen azmin ve sabrın sonucu olarak ortaya çıkan yapıt başarıyı daha da yukarı seviyelere çıkaracaktır

    Machine Learning Research Trends in Africa: A 30 Years Overview with Bibliometric Analysis Review

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    In this paper, a critical bibliometric analysis study is conducted, coupled with an extensive literature survey on recent developments and associated applications in machine learning research with a perspective on Africa. The presented bibliometric analysis study consists of 2761 machine learning-related documents, of which 98% were articles with at least 482 citations published in 903 journals during the past 30 years. Furthermore, the collated documents were retrieved from the Science Citation Index EXPANDED, comprising research publications from 54 African countries between 1993 and 2021. The bibliometric study shows the visualization of the current landscape and future trends in machine learning research and its application to facilitate future collaborative research and knowledge exchange among authors from different research institutions scattered across the African continent

    Automatic Question Generation to Support Reading Comprehension of Learners - Content Selection, Neural Question Generation, and Educational Evaluation

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    Simply reading texts passively without actively engaging with their content is suboptimal for text comprehension since learners may miss crucial concepts or misunderstand essential ideas. In contrast, engaging learners actively by asking questions fosters text comprehension. However, educational resources frequently lack questions. Textbooks often contain only a few at the end of a chapter, and informal learning resources such as Wikipedia lack them entirely. Thus, in this thesis, we study to what extent questions about educational science texts can be automatically generated, tackling two research questions. The first question concerns selecting learning-relevant passages to guide the generation process. The second question investigates the generated questions' potential effects and applicability in reading comprehension scenarios. Our first contribution improves the understanding of neural question generation's quality in education. We find that the generators' high linguistic quality transfers to educational texts but that they require guidance by educational content selection. In consequence, we study multiple educational context and answer selection mechanisms. In our second contribution, we propose novel context selection approaches which target question-worthy sentences in texts. In contrast to previous works, our context selectors are guided by educational theory. The proposed methods perform competitive to related work while operating with educationally motivated decision criteria that are easier to understand for educational experts. The third contribution addresses answer selection methods to guide neural question generation with expected answers. Our experiments highlight the need for educational corpora for the task. Models trained on noneducational corpora do not transfer well to the educational domain. Given this discrepancy, we propose a novel corpus construction approach. It automatically derives educational answer selection corpora from textbooks. We verify the approach's usefulness by showing that neural models trained on the constructed corpora learn to detect learning-relevant concepts. In our last contribution, we use the insights from the previous experiments to design, implement, and evaluate an automatic question generator for educational use. We evaluate the proposed generator intrinsically with an expert annotation study and extrinsically with an empirical reading comprehension study. The two evaluation scenarios provide a nuanced view of the generated questions' strengths and weaknesses. Expert annotations attribute an educational value to roughly 60 % of the questions but also reveal various ways in which the questions still fall short of the quality experts desire. Furthermore, the reader-based evaluation indicates that the proposed educational question generator increases learning outcomes compared to a no-question control group. In summary, the results of the thesis improve the understanding of the content selection tasks in educational question generation and provide evidence that it can improve reading comprehension. As such, the proposed approaches are promising tools for authors and learners to promote active reading and thus foster text comprehension
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