IMPROVED LICENSE PLATE LOCALIZATION ALGORITHM BASED ON MORPHOLOGICAL OPERATIONS

Automatic License Plate Recognition (ALPR) systems have become an important tool to track stolen cars, access control, and monitor traffic. ALPR system consists of locating the license plate in an image, followed by character detection and recognition. Since the license plate can exist anywhere within an image, localization is the most important part of ALPR and requires greater processing time. Most ALPR systems are computationally intensive and require a high-performance computer. The proposed algorithm differs significantly from those utilized in previous ALPR technologies by offering a fast algorithm, composed of structural elements which more precisely conducts morphological operations within an image, and can be implemented in portable devices with low computation capabilities. The proposed algorithm is able to accurately detect and differentiate license plates in complex images. This method was first tested through MATLAB with an on-line public database of Greek license plates which is a popular benchmark used in previous works. The proposed algorithm was 100% accurate in all clear images, and achieved 98.45% accuracy when using the entire database which included complex backgrounds and license plates obscured by shadow and dirt. Second, the efficiency of the algorithm was tested in devices with low computational processing power, by translating the code to Python, and was 300% faster than previous work

Analysis of lesion border segmentation using watershed algorithm

Automatic lesion segmentation is an important part of computer-based skin cancer detection. A watershed algorithm was introduced and tested on benign and melanoma images. The average of three dermatologists\u27 manually drawn borders was compared as the benchmark. Hair removing, black border removing and vignette removing methods were introduced in preprocessing steps. A new lesion ratio estimate was added to the merging method, which was determined by the outer bounding box ratio. In postprocessing, small blob removing and border smoothing using a peninsula removing method as well as a second order B-Spline smoothing method were included. A novel threshold was developed for removing large light areas near the lesion boundary. A supervised neural network was applied to cluster results and improve the accuracy, classifying images into three clusters: proper estimate, over-estimate and under-estimate. Comparing to the manually drawn average border, an overall of 11.12% error was achieved. Future work will involve reducing peninsula-shaped noise and looking for other reliable features for the classifier --Abstract, page iii

Machine vision system for weed detection using image filtering in vegetables crops

This work presents a machine vision system for weed detection in vegetable crops using outdoor images, avoiding lighting and sharpness problems during acquisition step. This development will be a module for a weed removal mobile robot with camera obscura (Latin for “dark room”) for lighting controlled conditions. The purpose of this paper is to develop a useful algorithm to discriminate weed, using image filtering to extract color and area features, then, a process to label each object in the scene is implemented, finally, a classification based on area is proposed, including sensitivity, specificity, positive and negative predicted values in order to evaluate algorithm performance

Label-free imaging flow cytometry for analysis and sorting of enzymatically dissociated tissues

Biomedical research relies on identification and isolation of specific cell types using molecular biomarkers and sorting methods such as fluorescence or magnetic activated cell sorting. Labelling processes potentially alter the cells’ properties and should be avoided, especially when purifying cells for clinical applications. A promising alternative is the label-free identification of cells based on physical properties. Sorting real-time deformability cytometry (soRT-DC) is a microfluidic technique for label-free analysis and sorting of single cells. In soRT-FDC, bright-field images of cells are analyzed by a deep neural net (DNN) to obtain a sorting decision, but sorting was so far only demonstrated for blood cells which show clear morphological differences and are naturally in suspension. Most cells, however, grow in tissues, requiring dissociation before cell sorting which is associated with challenges including changes in morphology, or presence of aggregates. Here, we introduce methods to improve robustness of analysis and sorting of single cells from nervous tissue and provide DNNs which can distinguish visually similar cells. We employ the DNN for image-based sorting to enrich photoreceptor cells from dissociated retina for transplantation into the mouse eye

Cancer diagnosis using deep learning: A bibliographic review

In this paper, we first describe the basics of the field of cancer diagnosis, which includes steps of cancer diagnosis followed by the typical classification methods used by doctors, providing a historical idea of cancer classification techniques to the readers. These methods include Asymmetry, Border, Color and Diameter (ABCD) method, seven-point detection method, Menzies method, and pattern analysis. They are used regularly by doctors for cancer diagnosis, although they are not considered very efficient for obtaining better performance. Moreover, considering all types of audience, the basic evaluation criteria are also discussed. The criteria include the receiver operating characteristic curve (ROC curve), Area under the ROC curve (AUC), F1 score, accuracy, specificity, sensitivity, precision, dice-coefficient, average accuracy, and Jaccard index. Previously used methods are considered inefficient, asking for better and smarter methods for cancer diagnosis. Artificial intelligence and cancer diagnosis are gaining attention as a way to define better diagnostic tools. In particular, deep neural networks can be successfully used for intelligent image analysis. The basic framework of how this machine learning works on medical imaging is provided in this study, i.e., pre-processing, image segmentation and post-processing. The second part of this manuscript describes the different deep learning techniques, such as convolutional neural networks (CNNs), generative adversarial models (GANs), deep autoencoders (DANs), restricted Boltzmann’s machine (RBM), stacked autoencoders (SAE), convolutional autoencoders (CAE), recurrent neural networks (RNNs), long short-term memory (LTSM), multi-scale convolutional neural network (M-CNN), multi-instance learning convolutional neural network (MIL-CNN). For each technique, we provide Python codes, to allow interested readers to experiment with the cited algorithms on their own diagnostic problems. The third part of this manuscript compiles the successfully applied deep learning models for different types of cancers. Considering the length of the manuscript, we restrict ourselves to the discussion of breast cancer, lung cancer, brain cancer, and skin cancer. The purpose of this bibliographic review is to provide researchers opting to work in implementing deep learning and artificial neural networks for cancer diagnosis a knowledge from scratch of the state-of-the-art achievements