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Mutations in <i>RPS3</i> affect cleavage efficiency independent of stall sequence.

By Carrie L. Simms (6008843), Kyusik Q. Kim (6008846), Liewei L. Yan (6008849), Jessica Qiu (3925493) and Hani S. Zaher (2522782)

Abstract

<p>Northern analysis of 5’ fragments accumulation from <i>ski2Δ</i> strains, with and without mutations in <i>RPS3</i>, carrying different stalling reporters. PGK1 contains a PGK1 mRNA without any additional sequence; SL contains a stem loop at position 1040 of PGK1; (CGA)<sub>12</sub>, (AAA)<sub>12</sub>, and (UUU)<sub>12</sub> indicate the corresponding codons were inserted at position 950 of PGK1. Corresponding ethidium-bromide stained agarose gel is shown (bottom panel).</p

Topics: Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Infectious Diseases, Biological Sciences not elsewhere classified, NGD, residue, rps 3 mutations, entry tunnel, ribosome quality control, Rps 3, endonucleolytic cleavage reaction, mRNA, protein Rps 3, RQC, no-go decay No-go Decay
Year: 2018
DOI identifier: 10.1371/journal.pgen.1007818.g003
OAI identifier: oai:figshare.com:article/7382144
Provided by: FigShare
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