Mutations in <i>RPS3</i> affect cleavage efficiency independent of stall sequence.
<p>Northern analysis of 5’ fragments accumulation from <i>ski2Δ</i> strains, with and without mutations in <i>RPS3</i>, carrying different stalling reporters. PGK1 contains a PGK1 mRNA without any additional sequence; SL contains a stem loop at position 1040 of PGK1; (CGA)<sub>12</sub>, (AAA)<sub>12</sub>, and (UUU)<sub>12</sub> indicate the corresponding codons were inserted at position 950 of PGK1. Corresponding ethidium-bromide stained agarose gel is shown (bottom panel).</p
Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Infectious Diseases, Biological Sciences not elsewhere classified, NGD, residue, rps 3 mutations, entry tunnel, ribosome quality control, Rps 3, endonucleolytic cleavage reaction, mRNA, protein Rps 3, RQC, no-go decay No-go Decay
DOI identifier: 10.1371/journal.pgen.1007818.g003
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