Mutations in <i>RPS3</i> affect cleavage efficiency independent of stall sequence.

Abstract

Northern analysis of 5’ fragments accumulation from ski2Δ strains, with and without mutations in RPS3, carrying different stalling reporters. PGK1 contains a PGK1 mRNA without any additional sequence; SL contains a stem loop at position 1040 of PGK1; (CGA)12, (AAA)12, and (UUU)12 indicate the corresponding codons were inserted at position 950 of PGK1. Corresponding ethidium-bromide stained agarose gel is shown (bottom panel).</p