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LC treatment selectively induces expression of p21<sup>cip1</sup> gene, mRNA and protein in cancer cells.

By Hongbiao Huang (292949), Ningning Liu (292952), Haiping Guo (292959), Siyan Liao (292957), Xiaofen Li (292961), Changshan Yang (292954), Shouting Liu (292962), Wenbin Song (292969), Chunjiao Liu (292965), Lixia Guan (292963), Bing Li (43671), Li Xu (108702), Change Zhang (292967), Xuejun Wang (174299), Q. Ping Dou (141563) and Jinbao Liu (292973)


<p>(<b>a</b>) LC induces p21<sup>cip1</sup> gene expression but not p27 and GAPDH. HepG2 cells were treated with LC (2.5, 5.0, 10 mM) for 24 h; cells were collected for gene expression profile analysis. In the gene chip, there are 2 probes for p21<sup>cip1</sup> and 1 probe for p27<sup>kip1</sup>. All the fold increases of p21<sup>cip1</sup> and p27<sup>kip1</sup> gene expression <i>versus</i> control were shown. (<b>b</b>) LC dose-dependently induces p21<sup>cip1</sup> mRNA expression but not p27<sup>kip1</sup> in HepG2 cells. HepG2 cells were incubated with different concentrations of LC (2.5, 5, 10 mM) for either 12 h or 24 h; the cells were collected for mRNA assay of p21<sup>cip1</sup> and p27<sup>kip1</sup> by real-time PCR. Fold increase of the LC-treated <i>versus</i> control was shown. Mean+SD (nā€Š=ā€Š3). *<i>P</i><0.01, **<i>P</i><0.05, compared with control. (<b>c</b>) LC dose-dependently and time-dependently induces p21<sup>cip1</sup> protein accumulation in HepG2 cancer cells. HepG2 and SMMC7721 cells were treated with various doses of LC for 48 h or HepG2 cells were exposed to 5 mM of LC for 12, 24, 36, 48 h; p21 and p27 proteins were detected by Western blot. (<b>d</b>) LC dose-dependently decreases Rb phosphorylation. HepG2 cells were treated with LC for 48 h; Rb and phosphorylated Rb were dectected by Western blot. Typical Western images were shown (left) and band intensity was quantified (right).</p

Topics: Genetics, Molecular Biology, Biological Sciences, Cancer, selectively, induces, mrna, cancer
Year: 2013
DOI identifier: 10.1371/journal.pone.0049062.g003
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Provided by: FigShare
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