<p>(<b>a</b>) LC induces p21<sup>cip1</sup> gene expression but not p27 and GAPDH. HepG2 cells were treated with LC (2.5, 5.0, 10 mM) for 24 h; cells were collected for gene expression profile analysis. In the gene chip, there are 2 probes for p21<sup>cip1</sup> and 1 probe for p27<sup>kip1</sup>. All the fold increases of p21<sup>cip1</sup> and p27<sup>kip1</sup> gene expression <i>versus</i> control were shown. (<b>b</b>) LC dose-dependently induces p21<sup>cip1</sup> mRNA expression but not p27<sup>kip1</sup> in HepG2 cells. HepG2 cells were incubated with different concentrations of LC (2.5, 5, 10 mM) for either 12 h or 24 h; the cells were collected for mRNA assay of p21<sup>cip1</sup> and p27<sup>kip1</sup> by real-time PCR. Fold increase of the LC-treated <i>versus</i> control was shown. Mean+SD (n = 3). *<i>P</i><0.01, **<i>P</i><0.05, compared with control. (<b>c</b>) LC dose-dependently and time-dependently induces p21<sup>cip1</sup> protein accumulation in HepG2 cancer cells. HepG2 and SMMC7721 cells were treated with various doses of LC for 48 h or HepG2 cells were exposed to 5 mM of LC for 12, 24, 36, 48 h; p21 and p27 proteins were detected by Western blot. (<b>d</b>) LC dose-dependently decreases Rb phosphorylation. HepG2 cells were treated with LC for 48 h; Rb and phosphorylated Rb were dectected by Western blot. Typical Western images were shown (left) and band intensity was quantified (right).</p
Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.