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Differential subcellular localization of wild type and mutant ACE.

By Sergei M. Danilov (118956), Sergey Kalinin (342870), Zhenlong Chen (342867), Elena I. Vinokour (365163), Andrew B. Nesterovitch (342866), David E. Schwartz (118944), Olivier Gribouval (365164), Marie-Claire Gubler (365165) and Richard D. Minshall (342873)


<p>Confocal microscopy was used to determine the localization of WT- vs. Q1069R-ACE mutant expressed in CHO cells following immunostaining with anti-ACE mAb 9B9 and Alexa 488 goat anti-mouse secondary Ab. A-C: WT-ACE expressing cells; D-F: Q1069R-ACE expressing cells. A,B and D,E. Immunostaining of WT and Q1069R ACE in 0.3% paraformaldehye for 15 min at 4C (fixed only). B and E. Treatment with the chemical chaperone sodium butyrate and proteosome inhibitor MG132 for 24 hrs followed by immunostaining with 9B9 mAb. C and F. CHO cells fixed and permeabilized with 3% paraformaldehyde for 15 min at 37C and labeled as above show WT-ACE on the plasma membrane and to a lesser extent in the cytosol and Q1069R-ACE in perinuclear areas.</p

Topics: Biochemistry, Medicine, Cell Biology, Genetics, Pharmacology, Virology, subcellular, localization, mutant
Year: 2013
DOI identifier: 10.1371/journal.pone.0010438.g004
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Provided by: FigShare
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