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TripleQuad MRM-MS detection of 4-MU.

By Claudia Cozma (766350), Sabrina Eichler (766351), Gyula Wittmann (766352), Alba Flores Bonet (766353), Guido Johannes Kramp (766354), Anne-Katrin Giese (440558) and Arndt Rolfs (140849)

Abstract

<p>Hydroxyl-chromen-2H-one compounds can be detected in Q1 scan as a (M-H)<sup>-</sup> ion and, under specific collision energy, the hetero-cycle is broken with a neutral loss of the fragment containing–COO•. We propose that the remaining fragment undergoes a molecular rearrangement to obtain a more stable structure. For enzymatic product detection and quantification, two transitions are monitored: 175/119 (4-MU) and 217/160 (internal standard). <b>A.</b> Collision induced dissociation fragmentation spectrum (MS<sup>2</sup>) of the analyte (4-MU), obtained using an ABSciex 5500, and MRM-MS transition spectrum monitored during the MPS IV assays (175/119); B. Collision induced dissociation fragmentation spectrum (MS2) of the internal standard (4-propyl-5-hydroxy-7-methyl-2H-chromen-2-one), obtained using an ABSciex 5500, and MRM-MS transition spectrum monitored during the MPS IV assays (217/160).</p

Topics: Biological Sciences, assay, autosomal recessive disease, dbs, Enzyme Replacement Therapy, MPS IVA, MPS IVA patients, GALNS, MPS IVA diagnosis, blood spots, lc, validation blood samples
Year: 2015
DOI identifier: 10.1371/journal.pone.0131228.g001
OAI identifier: oai:figshare.com:article/1476128
Provided by: FigShare
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