UVA Assisted 4-Thiothymidine for Cancer Treatment

This article reviews the developments of 4-thiothymidine analogues, assisted with UVA light, as a novel cancer therapy. First, the key points on synthetic chemistry, photochemistry and cellular toxicity of 4-thiothymidine are summarized. As the chemical structure of 4-thiothymidine is very similar to that of its parent thymidine, thus 4-thiothymidine can be readily incorporated into cellular DNA, and with the help of thymidine kinase, much more preferably into cancerous DNA. Unlike thymidine, 4-thiothymdine can absorb strongly in UVA (longer wavelengths of UV) light. Thus UVA-assisted 4-thiothymidine offers an effective cancer treatment. Some underlying mechanisms of action by 4-thiothymidine/UVA and compares this cancer approach with the commonly used photodynamic therapy are discussed. The various interactions between 4-thiothymidine with human serum albumin are introduced. Finally, a short conclusion on the past efforts and a brief prospect for future work in this exciting research field are given

Influence of Root Secretion Flavonoids on the Biogeochemistry Behavior of Cadmium in Avicennia marina (Forsk.) Vierh Rhizosphere

红树林生态系统主要分布于热带和亚热带的海岸潮间带，其具有独特的生态学和生物学特性，为污染物的吸附治理提供了最佳场所。然而，近年来，随着工业化、城市化和地区经济的快速发展，导致大量生活污水和工业废水流向红树林湿地，使得红树林生态系统面临越来越严重的重金属污染，从而使得滨海湿地面临着日益严峻的重金属污染。进入红树林湿地的污染物最先在红树植物根际区进行一系列形态转化等过程，而后被植物吸收累积。因此，红树林根际区重金属化学行为已经成为近年来污染生态学的研究热点。 类黄酮是植物体内广泛存在的一类重要次生代谢产物，是一种常见的根系分泌物，对植物缓解胁迫、抵御逆境有着重要的生理生态作用。目前对红树林生态系...Mangrove ecosystem is widely distributed in tropical and subtropical coastal intertidal zone, and it provides the best places for adsorption of pollutants and governance of environment because of its unique biological and ecological characteristics. However, a large amount of domestic and industrial sewage discharge into mangrove with rapid development of urbanization, industrialization and region...学位：理学博士院系专业：环境与生态学院_生态学学号：3312012015356

Preparation and Application of Monoclonal Antibody against Puerarin

葛根为豆科植物野葛或粉葛的干燥根，为常用中药之一，具有解肌退热、生津、透疹、升阳止泻的功效，多用于治疗外感发热、头痛、项强、消渴、麻疹不透、热痢、泄泻、高血压等症状。葛根素(Puerarin，PU)是葛根的主要活性成分，属于黄酮类化合物，药理作用广泛，是一种很有开发前景的药物。 本实验采用杂交瘤技术制备葛根素的单克隆抗体，在此基础上建立葛根素的酶联免疫吸附分析法检测技术，对葛根中药及中成药中葛根素的含量进行检测和评价。 主要的实验结果如下： （1）采用高碘酸钠法合成免疫人工抗原PU-BSA和包被人工抗原PU-HSA，经MALDI-TOF-MS鉴定，载体蛋白连接的半抗原数分别为18和16个...Pueraria is the dry root of Pueraria lobata (wild) Ohwi or Pueraria thomsonii Benth, one of characteristic traditional Chinese medicines, has many pharmaceutical effects, such as anti-inflammatory, reducing blood sugar, blood pressure, and so on. Pharmaceutical researches indicated that puerarin, an isoflavone, is the major pharmaceutical ingredient of herbal Pueraria, functioning lots of bio-medi...学位：理学硕士院系专业：生命科学学院_细胞生物学学号：2172009115218

小鱼仙草精油化学成分的GC-MS分析

福建省科委基金资助项目 (K20096);福建省自然科学基金项目;国家中医药管理局项目 (2000-J-P-40

Observation on the adverse reactions of different concentrations of povidone-iodine applied before cataract surgery

AIM: To evaluate the efficiency and safety of 50g/L povidone-iodine solution in preventing postoperative endophthalmitis through comparing the incidence of postoperative endophthalmitis and adverse reactions after conjunctival sac washing with povidone-iodine of different concentrations.<p>METHODS: Totally 500 cataract patients were divided into 50g/L povidone-iodine group and 25g/L povidone-iodine group. All the operated eyes were observed during and after surgery.The patients' subjective discomfort was inquired and their signs of eyes were recorded. <p>RESULTS: The eye irritation of 50g/L povidone-iodine group was more significant than 25g/L povidone-iodine group. No significant difference in the corneal epithelial loss and endophthalmitis was observed between two groups.<p>CONCLUSION:Conjunctival sac washing with 50g/L povidone-iodine is an effective and safe measure to prevent endophthalmitis after cataract surgery

液滴传质过程Marangoni对流的引发

对在丙酮-甲苯-水体系中由于传质而引发的Marangoni效应进行数值模拟,比较了二维和三维模型模拟计算结果的差异,主要考察丙酮相对质量浓度、界面平均流动速度等参数在传质过程中的变化。结果表明,两种模型计算结果差异较大,三维模型中Marangoni对流引发的更加充分,更贴近于实际情况。随后在三维模型中,分别考察了Gibbs吸附层与丙酮初始质量浓度对传质的影响,与文献结果对比发现液-液界面中Gibbs吸附层的影响可以忽略,而丙酮初始质量浓度越高,Marangoni对流的引发时间越早,强度越强

Functions of DHP and its nuclear receptor on reproductive neuroendocrine in zebrafish (Danio rerio)

本文研究了DHP对野生型和孕酮核受体敲除纯合型两种类型雄性斑马鱼垂体中促性腺激素β亚基基因（fshb和lhb）、脑中多巴胺合成限速酶酪氨酸羟基化酶基因（th2）及多巴胺二型受体基因（drd2a和drd2b）和促性腺激素释放激素基因（gnrh2和gnrh3）mRNA表达的影响。主要研究结果如下： 1.野生型雄性斑马鱼垂体中促性腺激素β亚基基因（fshb和lhb）mRNA表达呈现周日变化趋势，可分为基础表达期、上升表达期和高峰表达期3个期，高峰表达期的mRNA表达量显著高于其它2个期。野生型雄性斑马鱼活体暴露于DHP水溶液后，垂体中fshb和lhbmRNA表达量随DHP暴露浓度的增加和暴露时间的...We examined the effects of DHP on the fshb, lhb, th2, drd2a, drd2b, gnrh2 and gnrh3 mRNA expression in WT and pgr-/-. The main results were as follows: 1. fshb and lhb mRNA expression of WT showed diurnal change. The level of peak expression phase was significantly higher than those of other two expression phases. After WT were exposed to DHP in vivo, fshb and lhb mRNA expression showed a upward ...学位：理学博士院系专业：海洋与地球学院_海洋生物学学号：2232012015345

Over-expression of Xylose isomerase/Xylulokinase and Construction of O2-tolerant hydrogenase in Klebsiella oxytoca HP1

在石化燃料枯竭和全球气候变暖的双重危机下，氢气作为一种可再生、零排放和高热值的清洁能源载体而备受关注。关于氢能的研究主要围绕氢的制取和氢的利用两个方面展开。在众多制氢方式中，生物制氢因其生产成本低、原料来源丰富和环境污染小，从而成为制氢研究的热点。在氢的利用中，燃料电池技术一直以来都是研究的重点。氢酶是一种含金属的氧化还原酶，其广泛存在于微生物中，能够可逆地催化H+的还原和H2的氧化(2H++2e-&#8596;H2)。无论是在氢的制取还是氢的利用中，氢酶都是氢能技术的核心之一。 KlesiellaoxytocaHP1具有高效产氢的特性，且生长迅速，可用于大规模发酵产氢。纤维素是世界上最丰富...Facing energy crisis and global warming, the clean energy carrier H2 has been a hot spot with characters of renewability, zero-emission and high energy yield. Research on hydrogen energy mainly focuses on hydrogen production and hydrogen application. Among all hydrogen production modes, biohydrogen production has become a promising method because of its low cost, abundant source of raw materials a...学位：理学硕士院系专业：生命科学学院_生物化学与分子生物学学号：2162012115231

Synthesis of Bis-β-diketonate Rare Earth Complexes with Azobenzene Bridged and Studies of Their Photo / Thermal Response Properties

稀土β-二酮配合物是一类最受广泛研究的稀土配位化合物的分子基材料。这主要基于β-二酮配体作为一种经典的螯合配体，通过烯醇式与稀土金属离子进行配位，可以螯合形成稳定的六元环而赋予动力学活性的稀土配合物具有一定的稳定性，且由于β-二酮配体中具有高摩尔吸收系数，能与中心稀土离子之间发生高效能量传递，使得配合物当有紫外光照射时能发出稀土离子的特征荧光，从而稀土β-二酮类配合物在荧光器件材料、磁学材料、催化、分子识别与吸附等方面具有广泛的应用前景。因此，对新型稀土β-二酮配合物的开发及其性能研究具有重要的实际意义。 本文在双β-二酮配体中引入具有光响应性质的偶氮苯基团，构建具有光响应性质的双核稀土β-...The rare earth β-diketonate complex is one of the most widely studied molecular-based materials. This is mainly based on the β-diketone ligand as a classical chelate ligand, it can chelate with rare earth metal ions to form a stable six-membered ring by enol form and to give kinetic lability of rare earth complexes with certain stability. In the rare earth β-diketonate complexes, the β-diketone li...学位：理学硕士院系专业：化学化工学院_无机化学学号：2052014115150

The effection of regulate AQP-4 on hydrocephalus in rats

目的：研究调控AQP-4蛋白对大鼠脑积水发生发展的治疗作用。 方法：雄性成年（周龄约8-9周）SD大鼠（250±20g），随机分成4组，脑积水组20只，生理盐水组（假手术组）20只，阴性干预组（予以腹腔注射黄体酮）20只，阳性干预组（予以腹腔注射睾酮）20只。脑积水模型建立方法为：腹腔注射水合氯醛麻醉SD大鼠后，固定于脑立体定位仪上，以微量注射泵调节微量进样器向大鼠脑室内注射30μl的3%高岭土混悬液，使之产生脑积水。生理盐水组的大鼠脑室内注射为生理盐水，其余的脑积水组、阴性干预组、阳性干预组则同用脑积水模型建立方法。造模1周后，对脑积水组不行任何处理，对阴性干预组予以腹腔注射黄体酮，对阳性...Objective: To study the effection of regulate AQP-4 protein on hydrocephalus’ development in rats Methods: Male adult (about 8 weeks to 9 weeks) Sprague-Dawley rats (250±20g), were randomly divided into four groups, hydrocephalus group (n=20), the saline group (sham group) (n=20) and negative in the intervention group ( intraperitoneal injection of progesterone) (n=20), positive intervention gro...学位：医学硕士院系专业：医学院_外科学学号：2452012115318