The functional modification of PEGylated brush-polymer by peptides and their interaction mechanism with phospholipid membrane

刷状聚合物的合成和在医学治疗的应用是目前纳米生物分子材料领域研究的热点。同时PEG化后的刷状聚合物更有优良的生物性能，兼具多方面的优势，因而被应用于多肽/蛋白质药物的功能化修饰、药物可控递送体系以及生物传感等方面。但同时这些材料在其应用中也面临着与生物磷脂膜的作用能力弱和进细胞效率低的问题。因此，我们需要对材料表面进行电荷性质和疏水性质的调控以实现与磷脂膜的作用增强和进细胞效率的提高，并更为深入研究其作用机制。本论文旨在通过构建不同氨基酸序列多肽功能化修饰的PEG类刷状聚合物形成的多肽-聚合物结合物的体系，以实现对PEG类刷状聚合物表面电荷性质和疏水性质的调控，并在此体系的基础上研究电荷性质和...The synthesis and application in medical treatment of the brush-polymer are the hotspot in the research of nano biological molecules. At the same time, PEGylated brush-polymers have more excellent biological performance as well as advantages, and can be applied to functional modification of peptide/protein drugs, drug controlled delivery system and biological sensing, etc. But these materials in t...学位：理学硕士院系专业：化学化工学院_分析化学学号：2052013115156

Microwave Synthesis of Upconversion Luminescence and Magnetic Nanomaterials and Their Application in Biosensing

摘要 本论文以稀土掺杂上转换发光纳米晶和尖晶石型铁氧体磁性纳米粒子这两类纳米材料为研究主体，力图从制备方法、结构与性能关系及其在生物医学检测等几方面都有所创新性的研究。主要内容概括如下： 第一章，综述了微波加热的原理及其在无机纳米材料可控合成中的优势与应用，上转换发光的基本原理、磁性纳米材料的磁学特性以及这两类材料在生物医学中的应用研究进展。 第二章，结合微波加热与“乙醇/水”溶剂体系，发展了一种快速、便捷的NaGdF4:Yb,Er/Tm/Ho上转换发光/顺磁性纳米晶制备方法。通过对照实验，证实微波加热不仅非常显著地把传统热传导加热条件下所需的数小时保持时间缩短到5min以内，而且产物的...Abstract In this thesis, rare earth doped upconversion luminescence nanoparticles (UCNPs) and spinel ferrite magnetic nanoparticles (MNPs) are choosen as research objects. The project mainly focuses on the preparation process, the relationship between structure & properties and the application in biomedical detection. The main contents are as follow. In the first chapter, the mechanism of microw...学位：理学博士院系专业：材料学院_高分子化学与物理学号：2072011015349

Research on the Effects of Pulsed Field on Cell and The Cellular Dielectric Parameters

近年来，随着电子和通讯技术的进步，电磁场对生命体的影响越来越受人们的关 注。其中，脉冲电场对细胞结构和功能的影响与对肿瘤细胞的消融作用逐渐成为近些 年生物电磁领域研究的热点。在脉冲电场作用下，细胞的形状，内部结构的电磁性质 会发生改变，甚至可以靶向性诱导肿瘤细胞凋亡、坏死。研究脉冲电场作用下细胞的 电响应及细胞内部导电、介电性质的改变对揭示电磁场生物效应的发生机制及肿瘤治 疗有着非常重要的意义。为此，本论文着眼于电磁场与细胞的相互作用，深入研究了 脉冲电场下细胞穿孔和细胞融合的机理、细胞内部结构介电参数的测试及带电纳米粒 子介入后细胞内的微观电磁效应。具体内容如下： 第一章，电...Recently, with the improvement of electronic and communication technologies, the effects of electromagnetic on human beings have attracted a great deal of attention. In particular, the effects of pulse field on cellular structure and function and ablation of tumor cells have been emerged as hot topics in the field of bioelectromagnetics in recent years. The shape and the electromagnetic proper...学位：工学博士院系专业：物理科学与技术学院_物理电子学学号：3312013015421

2种钼簇化合物对酪氨酸酶的抑制作用及对凡纳滨对虾的保鲜效果研究

通过合成2种高核钼簇（NH4）12[Mo36O108（NO）4（H2O）16].33H2O(Mo36)和[Mo36O112（OH2）16（H2bipy）4].28H2O (Mo36-bipy),其对酪氨酸二酚酶活性的抑制作用机理以及对凡纳滨对虾的防黑变保鲜效果进行研究。结果表明Mo36和Mo36-bipy对酪氨酸二酚酶IC50值分别为0.0358 mmol/L和0.0601 mmol/L,Mo36对酪氨酸酶的抑制作用为可逆竞争性抑制,Mo36-bipy对酪氨酸酶的抑制作用为可逆混合性抑制,且Mo36对酪氨酸酶活性的抑制作用效果优于Mo36-bipy。将凡纳滨对虾在4℃环境下贮藏10 d,通过对感官评分、颜色、pH值、TVB-N和菌落总数等指标检测,发现高核钼簇对样品具有一定保鲜效果。其均能适当提升感官分值、L*值,降低pH值与菌落总数。本研究结果为开发一种新型的抗虾体黑变的保鲜剂提供实验基础,并对多金属氧酸盐的应用研究拓展新的研究领域。国家自然科学基金项目(21871110

含镁离子电解质对氧化铝浆料流变性能的影响

通过高纯氧化铝陶瓷浆料粘度和zeta(ζ)电位等参数的考察,探讨了不同pH值下,含镁离子电解质对其流变性能的影响。结果表明,镁离子会降低氧化铝浆料的流变性能。在其存在下,无分散剂时与添加聚丙烯酸(PAA)作分散剂时,pH值分别为6.5和9.5的浆料具有良好的流变性能。无镁离子存在时,PAA的最佳添加量为0.5%;镁离子浓度为0.05mol/L时,该量增加至0.9%

用细胞电泳技术检测化疗药物电性

细胞电泳技术可用于研究氟尿嘧啶(5FU),甲氨喋呤(MTX)和环磷酸胺(CP)等药物的电性,用戊二醛化绵羊红细胞(SRBC)为载体,分别吸附3种化疗药物,均可以导致细胞表面电荷下降致使电泳缓慢率为:5 FU 14.55％,MTX 13.77％,CP 12.21％,这3种药物均带正电荷,此研究对电化学疗法结合化疗治疗恶性肿瘤有参考价值

Preparation and in Vitro Digestive Analysis of Casein-Derived Peptide-Zinc Chelates

In this study, casein peptides were obtained by alcalase hydrolyzation and Lactobacilus fermentation to prepare the casein peptide-zinc chelates, which are a kind of efficient and well-absorbed zinc supplement. The structure of casein peptide-zinc chelates was characterized by spectroscopic instruments. The digestive stability and safety were also assayed using in vitro digestive model and Caco-2 cell experiment. The results indicated that the optimal conditions for preparing casein peptides were as follows: Volume of alcalase was 0.3% (w/v), pH of reaction system was 9.0, Lactobacilus fermentation time was 12 h. Under this condition, the peptide content was 142.39±0.95 mg/g, and the zinc chelation rate was 31.41%±0.97%. The chelation with zinc destroyed the structure of casein peptides leading to a loose surface structure. Spectroscopic analysis showed that Zn2+ can bind to the reactive groups on casein peptides, with the chelating sites being carboxyl oxygen, hydroxyl oxygen and amino groups. The results of in vitro digestion showed that casein peptide-zinc chelates possessed better solubility than that of zinc sulfate. After gastrointestinal digestion, the DPPH and ABTS+ scavenging ability of casein peptide-zinc chelates increased by 26.19%±3.30% and 71.96%±7.06%, respectively. However, the ferric ion reducing power of the casein peptide-zinc chelates decreased by 36.26%±2.80%. At the same time, the β-turn and random coil content of the casein peptide-zinc chelate decreases during digestion, the β-sheet structure increases and Zn2+ play a role in maintaining the peptide structure. Furthermore, the cytotoxicity test indicated that casein peptide-zinc chelates gastrointestinal digest were toxic to Caco-2 cells when the concentration exceeded 0.4 mg/mL. Finally, 15 and 13 dairy-derived peptides were identified from casein hydrolysate and casein peptide-zinc chelates, respectively, using mass spectrometry. These results will provide scientific evidence for the development and application of efficient casein peptide-zinc chelates

The Expression and Significance of Polysialic Acid and Trisialic Acid in Lung Cancer, Breast Cancer, Stomach Cancer and Intestinal Cancer

多聚唾液酸(Poly-sia)是以α2，8键连接唾液酸残基而形成的线性均一多聚的碳水化合物，在细胞间黏附、细胞迁移以及神经发育中发挥了重要的作用，研究表明PolySia的表达与某些癌症的恶性程度、浸润、转移相关，认为其可作为某些癌症判断预后的临床标志物，但目前尚缺乏其与胃癌、肠癌，乳腺癌的病理关系。三聚唾液酸（TriSia）是含有三个唾液酸残基的线性糖链，主要黏附于神经节苷脂和蛋白质上，由于缺少识别糖蛋白上TriSia的抗体，TriSia的合成和功能及其作用机制都尚属未知，其在肿瘤中的表达及其意义尚未见报道。本研究采用westernblot的方法来检测93例肺癌，52例乳腺癌，38例胃癌和72...Polysialic acid (PolySia) is a carbohydrate which composes of a linear homopolymer of a-2, 8-linked sialic acid residues and plays important roles in intercellular adhesion, cell migration, formation and remodeling of the neural system through regulating adhesive property of NCAM. PolySia has been reported to be correlated with tumor progression, invasion and metastasis. The PolySia can be a clini...学位：医学硕士院系专业：医学院临床医学系_肿瘤学学号：2452009115306

On-line Comprehensive Two-dimensional Normal-phase Liquid Chromatography × Reversed-phase Liquid Chromatography for Preparative Isolation of Peucedanum praeruptorum

本文旨在构建在线全二维制备正相液相色谱（NPLC）与反相液相色谱（RPLC)联用的系统与方法。针对在线正相液相色谱与反相液相色谱联用中流动相不兼容的问题，设计了全新的接口装置。该接口装置包括正相富集单元、反相富集单元以及连接二者的正反相切换单元，其中正相富集单元采用了新设计的气流辅助吸附（AAA）技术，可有效地除去正相液相色谱中的有机流动相，并将样品富集于正相（硅胶）富集柱上；正反相切换单元用于洗脱正相富集柱上的样品，并将其转移至下一单元，该部分还负责正相富集柱的再生；反相富集单元为流动相稀释-固相捕集接口，能将第一维正相富集柱的样品洗脱液进行稀释后，富集在反相富集柱上，并进一步将其送入反相液...A new on-line comprehensive preparative two-dimensional normal-phase liquid chromatography × reversed-phase liquid chromatography (2D NPLC × RPLC) system was developed for the separation of complicated natural products. It was based on the use of a silica gel packed medium-pressure column as the first dimension and an ODS preparative HPLC column as the second dimension. The two dimensions were con...学位：医学硕士院系专业：药学院_药物化学学号：3232012115310