Effect of organic and conventional cultivation techniques on yield, phenolic content, and sensory parameters in two carrot varieties

conference paperCarrots are one of the most important field grown vegetables in Ireland with a farm gate value of 16 million euros in 2007. They contain health promoting bioactive compounds including carotenoids, phenolics and polyacetylenes. Organically grown vegetables are often perceived as healthier and to have better flavour. The objective of this study was to determine levels of phenolics and flavonoids in organic and conventionally grown carrots, and to determine if they can be distinguished by taste.The Department of Agriculture, Fisheries and Food (FIRM 06/NITARFC6) is gratefully acknowledged for financial support of this wor

Soluble and insoluble-bound phenolics and antioxidant activity of various industrial plant wastes

The potential of selected industrial food wastes from juice and nut production including apple peel, apple pomace, pomegranate peel, pomegranate seed, chestnut shell, and black carrot pomace as resources for natural antioxidants was investigated. Soluble free and insoluble-bound phenolics were extracted from the wastes and analyzed for total phenolic and flavonoid contents, phenolic profile and antioxidant activity. Total phenolic and total flavonoid contents of wastes were positively correlated with their antioxidant activity. The highest total phenolic and antioxidant activity were determined in soluble fraction of pomegranate peel due to a significant amount of punicalagin derivatives. Pomegranate peel and seed had the most phenolics and flavonoids in soluble form while other wastes had more than 45% of total phenolics in insoluble-bound form. Chestnut shell showed more antioxidant activity in insoluble-bound fraction compared to that of its soluble fraction. These findings showed that not only soluble but also an insoluble-bound fraction of the industrial wastes has good potential for valorization as a source of natural antioxidants

Ocean acidification and the loss of phenolic substances in marine plants.

Rising atmospheric CO(2) often triggers the production of plant phenolics, including many that serve as herbivore deterrents, digestion reducers, antimicrobials, or ultraviolet sunscreens. Such responses are predicted by popular models of plant defense, especially resource availability models which link carbon availability to phenolic biosynthesis. CO(2) availability is also increasing in the oceans, where anthropogenic emissions cause ocean acidification, decreasing seawater pH and shifting the carbonate system towards further CO(2) enrichment. Such conditions tend to increase seagrass productivity but may also increase rates of grazing on these marine plants. Here we show that high CO(2) / low pH conditions of OA decrease, rather than increase, concentrations of phenolic protective substances in seagrasses and eurysaline marine plants. We observed a loss of simple and polymeric phenolics in the seagrass Cymodocea nodosa near a volcanic CO(2) vent on the Island of Vulcano, Italy, where pH values decreased from 8.1 to 7.3 and pCO(2) concentrations increased ten-fold. We observed similar responses in two estuarine species, Ruppia maritima and Potamogeton perfoliatus, in in situ Free-Ocean-Carbon-Enrichment experiments conducted in tributaries of the Chesapeake Bay, USA. These responses are strikingly different than those exhibited by terrestrial plants. The loss of phenolic substances may explain the higher-than-usual rates of grazing observed near undersea CO(2) vents and suggests that ocean acidification may alter coastal carbon fluxes by affecting rates of decomposition, grazing, and disease. Our observations temper recent predictions that seagrasses would necessarily be "winners" in a high CO(2) world

Nutritional and sensory evaluation of ready-to-eat salads during shelf life

The evolution of the antioxidant and sensory properties of six commercially available ready-to-eat salads, rocket, iceberg lettuce, baby lettuce, lamb’s lettuce, curly endive and radicchio, was studied throughout the shelf life. Both the storage under modified atmosphere and in unsealed pouch in a domestic refrigerator were considered and compared. Ascorbic acid, total phenolics content and antioxidant capacity were chosen as the most representative nutritional parameters for this purpose. The data obtained were analyzed by Principal Component Analysis (PCA). Radicchio, lamb’s lettuce and rocket showed an initial higher content of the quality parameters studied and demonstrated a better resistance to air exposure than the other salads, being ascorbic acid the most affected parameter. Modified atmosphere packaging demonstrated to be a very useful and reliable technology to extend nutritional and sensory properties during the shelf life period for all the vegetables studied

Comparison of phenolic and flavonoid content and antioxidant activity in vitro among potato varieties.

Conference paperDiets rich in fruits and vegetables have been associated with a lower incidence of cancer and heart disease, which may be related to the antioxidant activity of bioactive compounds present in these foods. Phenolic compounds are potent antioxidants in vitro, of which flavonoids are of particular interest for their potential positive impact on health. Potatoes are one of the most consumed vegetables in the world and therefore an important source of phenolics. In Ireland they are the third most important crop with a farm gate value of approximately 45 million euros annually. The aim of this work was to evaluate the antioxidant activity of methanolic solutions extracted from the skin and flesh of potato tubers against the stable radical DPPH and analyze its relationship to the content of total phenolics and total flavonoids.Teagasc Walsh Fellowship Programm

Nutritional and Defensive Chemistry of Three North American Ash Species: Possible Roles in Host Performance and Preference by Emerald Ash Borer Adults

Black ash (Fraxinus nigra), green ash (F. pennsylvanica), and white ash (F. americana) are the three most abundant ash species in the northeastern USA. We compared emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), adult performance and preference among seedlings of the three ash species, and then related performance and preference to foli- age nutritional quality and defensive compounds. Longevity of EAB adults reared on green and white ash was found to be greater than on black ash. EAB adult females also seemed to show feeding preference among the three species of ash trees because the total foliage area consumption was greater on green ash and white ash compared to black ash in dual-choice tests; however, the total mass of foliage consumed did not differ. The foliage of all ash species was high in nitrogen and in most macro- and micro-nutrients studied. The patterns of EAB performance and preference did not correspond to any of the individual chemical compounds tested (nitrogen, proteins, most macro- and micro-nutrients, or putative defensive compounds of ash seedlings). Never- theless, greater longevity of EAB adults on green and white ash compared to black ash was probably related to unbalanced nutrients (total nitrogen/total non-structural carbohydrate ratio) of black ash. Putative defensive compounds (i.e., phenolics and protease inhibitors) did not contribute to EAB longevity in this study, probably because (1) EAB adults were able to excrete most of these compounds and (2) their effects were alleviated by high nitrogen levels. More research is needed to elucidate the interactions of nitrogen and carbohydrate levels, and the interactions of nutrient balance and defensive plant allelochemicals on EAB performance and preference

A note on the effectiveness of selenium supplementation of Irish-grown Allium crops.

peer-reviewedThis study is funded by the Department of Agriculture and Food through the Network and Team Building Initiative of the Food Institutional Research Measure (FIRM Reference Number 06/NITARFC6).Onions and other Allium crops contain high levels of dietary phenolics and, unlike many other crops, accumulate the beneficial mineral selenium. Selenium-enhanced Allium crops are of interest both from a public good perspective and as a market positioning strategy for growers. Field trials were carried out to i) identify onion and scallion varieties that contain high levels of health-promoting phenolic and flavonoid compounds as potential targets for selenium supplementation and ii) investigate selenium supplementation in the widely-grown commercial onion variety ‘Hyskin’ at different application rates of nitrogen fertilizer. Levels of selenium in onion bulbs were significantly increased from 0.5–5.9 μg/g dry weight (DW) to 40.6–70.0 μg/g DW.Department of Agriculture, Food and the Marin

Change in Nutritional Quality of Detached Aspen and Willow Foliage Used as Insect Food in the Laboratory

Leaves of Populus tremuloides and Salix babylonica held in the laboratory as for feeding insects were analyzed for total nitrogen, total phenolics, and total sugars at 0, 3, and 6 days. More often than not, the three components varied due to leaf age, time since excision, or temperature. Temporal changes altered nutritional balance and probable impact on insect performance, Results suggest that other woody plant species would not remain nutritionally stable for long in the laboratory

Cell-wall polysaccharides play an important role in decay resistance of Sphagnum and actively depressed decomposition in vitro

Sphagnum-dominated peatlands head the list of ecosystems with the largest known reservoirs of organic carbon (C). The bulk of this C is stored in decomposition-resistant litter of one bryophyte genus: Sphagnum. Understanding how Sphagnum litter chemistry controls C mineralization is essential for understanding potential interactions between environmental changes and C mineralization in peatlands. We aimed to separate the effects of phenolics from structural polysaccharides on decay of Sphagnum. Wemeasured aerobic microbial respiration of different moss litter types in a lab. We used chemical treatments to step-wise remove the chemical compounds thought to be important in decay-resistance in three taxonomically distant moss genera. We also focused on the effect of Sphagnum-specific cell-wall pectin-like polysaccharides (sphagnan) on C and N mineralization. Removing polymeric lignin-like phenolics had only negligible effects on C mineralization of Sphagnum litter, but increased mineralization of two other bryophyte genera, suggesting a minor role of these phenolics in decay resistance of Sphagnum but a major role of cell-wall polysaccharides. Carboxyl groups of pectin-like polysaccharides represented a C-source in non-Sphagnum litters but resisted decay in Sphagnum. Finally, isolated sphagnan did not serve as C-source but inhibited C and N mineralization instead, reminiscent of the effects reported for phenolics in other ecosystems. Our results emphasize the role of polysaccharides in resistance to, and active inhibition of, microbial mineralization in Sphagnum-dominated litter. As the polysaccharides displayed decay-inhibiting properties hitherto associated with phenolics (lignin, polyphenols), it raises the question if polysaccharide- dominated litter also shares similar environmental controls on decomposition, such as temperature or nutrient and water availabilit