Binding of phage Φ29 protein p4 to the early A2c promoter: recruitment of a repressor by the RNA polymerase

Regulatory protein p4 from Bacillus subtilis phage Φ29 represses the early A2c promoter by binding upstream from RNA polymerase and interacting with the C-terminal domain of the RNA polymerase α subunit. This interaction stabilizes the RNA polymerase at the promoter in such a way that promoter clearance is prevented. Here, the binding of protein p4 to the A2c promoter has been studied. In the absence of RNA polymerase, protein p4 was found to bind with low affinity to a site centered at position −39 relative to the transcription start site. When RNA polymerase was present, protein p4 was displaced from this site and bound instead to a different target centered at position −71. Stable binding to this site requires the interaction of protein p4 with the C-terminal domain of the RNA polymerase α-subunit. Both sites contain sequences resembling the well-characterized p4 binding site present at the late A3 promoter, to which p4 binds with high affinity. A mutational analysis revealed that the site at −71 is critical for a stable interaction between protein p4 and RNA polymerase, and for efficient repression, whereas mutation of the site at −39 had only a small effect on repression efficiency. Therefore, RNA polymerase plays an active role in the repression mechanism by stabilizing the repressor at the promoter, generating a nucleoprotein complex that is too stable to allow promoter clearance.This investigation has been aided by Research Grant 5R01 GM27242-18 from the National Institutes of Health, by Grant PB93-0173 from Dirección General de Investigación Científica y Técnica, and by an Institutional Grant from Fundación Ramón Areces to the Centro de Biología Molecular “Severo Ochoa”. M. Monsalve and B.C. were holders of pre-doctoral fellowships from Comunidad Autónoma de Madrid and Ministerio de Educación y Ciencia, respectively.Peer reviewe