To study the influence on liver cancer by bear bile powder and its common content ursodeoxycholic acid

世界范围内，原发性肝癌（以下简称肝癌）在所有恶性肿瘤中，发病率第5位，死亡率第3位，其中半数以上发生于中国。肝癌通常发生于慢性肝病、肝硬化的基础上，大部分患者发现时已是中晚期肝癌，虽然治疗方法众多，但总体疗效不佳，亟需寻找更好的防治策略。一方面应高度重视乙肝疫苗接种等干预措施从根源上阻断肝癌，另一方面应积极开拓更好的治疗方法和源头创新药物。近年来中医药防治肝癌受到重视，因其整体观更强并能兼顾治疗基础性慢性肝病，提高患者的整体机能、改善生存质量、延长带瘤生存时间。 熊胆是传统名贵中药，因为保肝、利胆疗效显著，临床广泛应用于治疗各种慢性肝病，其主要成分熊去氧胆酸（UDCA）是FDA批准应用于治疗...Primary liver cancer (hereinafter referred to liver cancer) is a malignant tumor. The incidence was the fifth and the mortality was the third worldwide, and more than half of them occured in China. Liver cancer usually developed from chronic liver disease and cirrhosis. Most patients are diagnosed of hepatocellular carcinoma in late stage, though there are many kinds of treatments, the prognosis i...学位：理学博士院系专业：医学院_生理学学号：2452009015419

化疗药缓释制剂局部治疗肝癌的研究进展

本文综述了近年来应用抗癌药缓释剂对肝癌局部化疗的实验室及临床研究进展;并借鉴了其他实体肿瘤的研究成果,提出植入式缓释剂置入肝癌局部行间质化疗的新型给药途径

单用表阿霉素缓释剂及联合表阿霉素游离剂腹腔化疗治疗肝癌的疗效

目的观察单用表阿霉素(EPI)聚乳酸(PLA)缓释微球(MS)及联合游离表阿霉素(FEPI)腹腔化疗治疗肝癌的疗效。方法将35只H22腹水型肝癌荷瘤小鼠分为7组,每组5只,分别予空白微球,生理盐水,低、中、高剂量载药微球(分别含EPI 9、18、36 mg/kg),FEPI(9 mg/kg)和EPI-PLA-MS+FEPI(各含EPI 4.5 mg/kg),经腹腔注射给药,计算动物生命延长率。结果EPI-PLA-MS能有效延长荷瘤鼠的生存时间,并呈量效关系,小鼠最大耐受量约为18~36 mg/kg。联合用药组的平均生存时间、中位生存时间和生命延长率均达到最高值,分别为(40.0±16.9)d、33.5 d和222.58%。结论EPI-PLA-MS缓释剂与游离剂型联合腹腔化疗是一种新型、高效的给药方式,可有效治疗肝癌

脱氧核糖核酸在汞膜电极上的电化学行为

首次在汞膜电极上利用循环伏安、微分脉冲和交流伏安法研究脱氧核糖核酸（ＤＮＡ） 的电化学行为。结果表明汞膜电极作为一类固体电极可应用于负电位区ＤＮＡ电化学行为的 研究。同时结合凝胶电泳和ＵＶ光谱法，研究了用纯高氯酸处理的ＤＮＡ的氧化还原特性，结 果表明纯高氯酸可引起ＤＮＡ的变性和降解。纯高氯酸不适宜用于ＤＮＡ的变性处理

熊胆对二乙基亚硝胺诱发大鼠肝癌的影响

探讨熊胆对二乙基亚硝胺(diethylnitrosamine,DEN)诱发大鼠肝癌的影响。将大鼠随机分为正常组、模型组及熊胆两个剂量组(200、400 mg.kg 1,灌胃18周,每周5次)。DEN诱发肝癌,用熊胆粉干预。实验观察了体重等一般情况,检测肝功能,HE染色观察肝组织病理改变,免疫组化检测肝组织中增殖细胞核抗原(PCNA)和平滑肌动蛋白(α-SMA)。结果显示:4周后正常组体重高于其他各组(P<0.01),熊胆两组均高于模型组(P<0.05);除正常组外,其余各组谷丙转氨酶、总胆红素显著升高,但熊胆两组显著低于模型组;模型组呈典型肝硬化、肝癌改变,熊胆两组肝硬化程度较轻、癌组织分化较好;模型组高表达PCNA和α-SMA,熊胆两组较模型组弱,α-SMA减少具有显著意义。实验提示:熊胆在一定程度上抑制了DEN诱发肝癌的进程,可能与抑制肝星状细胞活化并减轻肝损害、肝硬化有关

Treatment of Hepatocellular Carcinoma in Mice with Locally Administered Epirubicin-loaded Poly(D,L)-lactic Acid Microspheres

[中文文摘]目的研究表柔比星-聚乳酸缓释微球(EPI-PLA-MS)局部给药治疗肝癌的效果。方法复乳-溶剂挥发法制备EPI-PLA-MS。将40只昆明小鼠随机分为5组,每组8只,分别腹腔注射不同剂量的游离表柔比星(FEPI),计算最大耐受量(MTD)。H22皮下实体瘤肝癌荷瘤小鼠3组,每组5只,分别用生理盐水(normalsaline,NS)、空白微球和含药微球(含EPI9mg/kg)瘤内注射给药,2周后取瘤称重。H22腹水型肝癌荷瘤小鼠3组,每组5只,分别用NS、空白微球和含药微球(含EPI9mg/kg)腹腔注射给药,计算动物生命延长率。结果FEPI腹腔注射的MTD为9mg/kg。EPI-PLA-MS瘤内给药后含药微球组和空白微球组的抑瘤率分别为40.35%和36.09%。腹腔给药后能显著延长荷瘤鼠的生存时间,含药微球组和空白微球组生命延长率分别为153.49%和142.22%。结论EPI-PLA-MS是一种有效低毒的药物新剂型,在局部治疗肝癌方面具有良好的临床应用前景。[英文文摘]Objective To study the effectiveness of treating hepatocellular carcinoma(HCC)in mice with locally administered epirubicin-loaded poly(D,L)-lactic acid microspheres(EPI-PLA-MS).Methods EPI-PLA-MS was prepared with double emulsion solvent evaporation technique.Five groups of mice(n=8 in each group)were intraperitoneally injected with five different doses of free epirubicin(FEPI),and the maximum tolerated dose(MTD)was calculated.Then 15 mice with transplanted subcutaneous H 22 HCC were divided into three group s ( n = 5) , which were respectively intratumorally injected with normal saline (NS) , blank micro-spheres, and EPI-PLA-MS (with 9mg/kg of EPI) 1 After two weeks the tumorswere excised and weighed1 Another 15 mice with transp lanted H22 ascites HCC were divided into three groups (n=5) , which were intraperitonealy injected with the same drugs, and the increased life span were registered exactly1 Resutls　TheMTD of intraperito neally injected FEPIwas 9mg/kg1 The tumour inhibiting rateswas 40135% and 36109% when EPI-PLA-MS were administered by intratumoral injection to the mice with subcutaneous H22 HCC1 It significantly p rolonged the survival time of mice with H22 ascites HCC and the increased life span by 153149% and 142122% when EPI-PLA-MS were intraperitoneally administered1 Conclusion　EPI-PLA-MS is a new sustained release preparation with high-ef-ficacy and low-toxicity in treating HCC and has shown promising prospects when administerd locally

Safety and Cost-Effectiveness Analysis of Laparoscopic Splenectomy by Secondary Pedicle Division Using Monopolar Electrocautery

Background/Aims: The expense of laparoscopic splenectomy (LS) has limited its use in developing countries, while medical costs are increasing worldwide. In this study, we performed LS by secondary pedicle division using monopolar electrocautery to achieve cost savings. Methodology: Over seven years, we performed 45 consecutive LSs by secondary pedicle division using monopolar electrocautery (n=17) or ultrasonic shears (n=28) at a single center. These were reviewed to assess outcome and cost. Results: Mean operating time was 179.7min, 7 Conversions to open operation (15.6%) were necessary. There were four postoperative complications (8.9%) and no deaths. Twenty-three of 28 (82.1%) patients with idiopathic thrombocytopenic purpura developed a long-term positive response; and mean operative cost was RMB6,577 (US$1,034), which was much lower than that of Endo-GIA (TM) in published reports. Between the monopolar electrocautery and ultrasonic shears groups, there were no significant differences in demographic characteristics or intraoperative and postoperative details, but operative cost was significantly lower in the former (RMB4,416, US$696 vs. RMB7,889, US$1,243; p<0.01). Conclusions: LS by secondary pedicle division using monopolar electrocautery is safe, efficacious and economical

控缓释制剂治疗肝癌新进展

控缓释制剂是一种新型药物剂型,与传统药物相比,达到了增效减毒的目的,对毒性大的抗肿瘤药意义非凡。近年来在肝癌研究方面取得了令人鼓舞的成果,拓宽了肝癌治疗尤其是化疗的思路。控缓释制剂包括多种形态,可经多途径给药应用于肝癌治疗

Chloride intracellular channel 1 is overexpression in hepatic tumor and correlates with a poor prognosis

Science and Technology Project of Xiamen, China [3502z20114012]; project of Xiamen health bureau, China [WSK 2010-02]Chloride intracellular channel 1 (CLIC1) is expressed in many human tissues and has been reported to be involved in the regulation of cell cycle, cell proliferation, and differentiation. Its roles in human hepatic tumor, however, remain unclear. The aim of this study was to investigate the clinicopathological significance and expression pattern of CLIC1 in human primary hepatic tumors. We examined the expression pattern of CLIC1 mRNA and protein in hepatic tumors using real-time quantitative RT-PCR and Western blot, respectively. CLIC1 protein and mRNA levels were significantly higher in cancerous tissues compared with corresponding normal tissue. In 85 hepatic tumor tissues, CLIC1 was significantly higher in 69 cases (81.2%), as determined by immunohistochemical staining. Increased CLIC1 expression was correlated with tumor size (p=0.021), distant metastasis (p=0.025), pathological TNM (pTNM) stage (p=0.023), and poor survival (25.11 +/- 2.27 vs 45.29 +/- 4.28months, p=0.001). Our data show that increased CLIC1 protein expression is associated with clinicopathological factors and a poor prognosis of hepatic tumors, and suggest that CLIC1 might represent a valuable prognostic marker for human hepatic tumors

Continuous regional arterial infusion and laparotomic decompression for severe acute pancreatitis with abdominal compartment syndrome

AIM: To evaluate the therapeutic effects of abdominal decompression plus continuous regional arterial infusion (CRAI) via a drug delivery system (DDS) in severe acute pancreatitis (SAP) patients with abdominal compartment syndrome (ACS)