Social representations of feeding people with diabetes mellitus

The aim of this study was to analyze the social representations of the diets 34 patients of the Brazilian public primary care service diagnosed with type 2 diabetes with DM2, according to how they represent their identity. Free association of words was used with responses to questions to survey social representations of food. Representations of identity were adopted from a previous study with the same participants. Normal participants eat healthily, eat little, eat greens and vegetables, avoid sweets, have positive attitudes, and maintain healthy eating habits and regular blood glucose levels. Participants who accept having the disease do not eat a lot, do not eat everything, avoid sweets, have an attitude of acceptance, and maintain inadequate eating habits and uncontrolled blood glucose. Participants with difficulties do not eat everything, do not eat a lot, do not follow the diet and eat vegetables and fruits, and have negative attitudes and altered blood glucose.info:eu-repo/semantics/publishedVersio

Social representations of feeding people with type-2 diabetes mellitus

The aim of this study was to analyze the social representations of the diets 34 patients of the Brazilian public primary care service diagnosed with type- 2 diabetes mellitus, according to how they represent their identity. Free association of words was used with responses to questions to survey social representations of food. Representations of identity were adopted from a previous study with the same participants. Normal participants eat healthily, eat little, eat greens and vegetables, avoid sweets, have positive attitudes, and maintain healthy eating habits and regular blood glucose levels. Participants who accept having the disease do not eat a lot, do not eat everything, avoid sweets, have an attitude of acceptance, and maintain inadequate eating habits and uncontrolled blood glucose. Participants with difficulties do not eat everything, do not eat a lot, do not follow the diet and eat vegetables and fruits, and have negative attitudes and altered blood glucose.info:eu-repo/semantics/publishedVersio

Toxoplasma effectors targeting host signaling and transcription

Early electron microscopy studies revealed the elaborate cellular features that define the unique adaptations of apicomplexan parasites. Among these were bulbous rhoptry (ROP) organelles and small, dense granules (GRAs), both of which are secreted during invasion of host cells. These early morphological studies were followed by the exploration of the cellular contents of these secretory organelles, revealing them to be comprised of highly divergent protein families with few conserved domains or predicted functions. In parallel, studies on host-pathogen interactions identified many host signaling pathways that were mysteriously altered by infection. It was only with the advent of forward and reverse genetic strategies that the connections between individual parasite effectors and the specific host pathways that they targeted finally became clear. The current repertoire of parasite effectors includes ROP kinases and pseudokinases that are secreted during invasion and that block host immune pathways. Similarly, many secretory GRA proteins alter host gene expression by activating host transcription factors, through modification of chromatin, or by inducing small noncoding RNAs. These effectors highlight novel mechanisms by whichhas learned to harness host signaling to favor intracellular survival and will guide future studies designed to uncover the additional complexity of this intricate host-pathogen interaction

Neutralizing antibody-independent immunity to SARS-CoV-2 in hamsters and hACE-2 transgenic mice immunized with a RBD/Nucleocapsid fusion protein [preprint]

The nucleocapsid (N) and the receptor binding domain (RBD) of the Spike (S) proteins elicit robust antibody and T cell responses either in vaccinated or COVID-19 convalescent individuals. We generated a chimeric protein that comprises the sequences of RBD from S and N antigens (SpiN). SpiN was highly immunogenic and elicited a strong IFNγ response from T cells and high levels of antibodies to the inactivated virus, but no neutralizing antibodies. Importantly, hamsters and the human Angiotensin Convertase Enzyme-2-transgenic mice immunized with SpiN were highly resistant to challenge with the wild type SARS-CoV-2, as indicated by viral load, clinical outcome, lung inflammation and lethality. Thus, the N protein should be considered to induce T-cell-based immunity to improve SARS-CoV-2 vaccines, and eventually to circumvent the immune scape by variants

Trypanosoma cruzi and its components as exogenous mediators of inflammation recognized through Toll-like receptors.

Trypanosoma cruzi is the etiologic agent of Chagas' disease, a parasitic disease of enormous importance in Latin America. Herein we review the studies that revealed the receptors from innate immunity that are involved in the recognition of this protozoan parasite. We showed that the recognition of T. cruzi and its components occurs through Toll-like receptors (TLR) 2/CD14. Further, we showed in vivo the importance of the myeloid differentiation factor (MyD88), an adapter protein essential for the function of TLRs, in determining the parasitemia and mortality rate of mice infected with T. cruzi. We also discuss the implications of these findings in the pathophysiology of Chagas' disease

Identity representation of users with diabetes Mellitus in primary care

As pessoas com diabetes mellitus tipo 2 (DM2) ao descobrirem a doença experimentam rupturas na identidade e nos comportamentos. Uma das formas de se aprofundar o conhecimento da visão subjetiva da pessoa com DM 2 é buscar suas representações identitárias, fenômeno dinâmico composto pelas representações de si-mesmo e representações intergrupo. Investigar a construção das representações identitárias dos usuários com DM2 da atenção primária à saúde e sua relação com as ações de autocuidado requeridas. Utilizou-se a associação livre de palavras, solicitando aos usuários com diabetes melittus tipo 2 de uma unidade básica de saúde (n=34) que sugerissem uma palavra ou expressão, com justificativa, após a questão indutora: quando se fala em eu sou diabético, o que vem à sua mente? Os discursos foram gravados, transcritos, categorizados e interpretados pela técnica da análise do conteúdo temático-categorial e as teorias da representação social e identidade social. As interações mediadas pelos processos identitários – comparação social, atribuição social e categorização no grupo estudado, proporcionaram a construção social das representações identitárias “normal”, “aceitar a doença”, “ser inconformado”, “ter dificuldades” dos participantes com DM2. Os profissionais da saúde devem compreender o contexto permeado pelo sentido subjetivo das pessoas com DM2 alterado pela doença, além do ponto de vista biomédico.People with type 2 diabetes mellitus (DM2) to discover the disease experience disruptions in identity and behavior. One of the ways to deepen the knowledge of the subjective view of the person with diabetes 2 is to seek their identity representations, dynamic phenomenon composed of representations of self and intergroup representations. To investigate the construction of the identity representations of users with DM2 of primary health care and its relationship with self-care actions required. We used the free word association, prompting users with diabetes mellitus type 2 in a basic health unit (n = 34) to suggest a word or phrase, with justification, after inducing question: when it comes to “I am diabetic, what comes to your mind?”. The speeches were recorded, transcribed, categorized and interpreted by analyzing the technique of thematic-categorical content and theories of social representation and social identity. The interactions mediated by identity processes – social comparison, social attribution and categorization in the study group, provided the social construction of identity representations “ normal”, “accepting the disease”, “being unaccepting, and “leading a difficult life”. Health professionals must understand the context permeated by subjective sense of people with DM2 changed by the disease, beyond the biomedical viewpoint.info:eu-repo/semantics/publishedVersio

Representações sociais das pessoas com diabetes mellitus: implicações no controle glicêmico

Pesquisas qualitativas envolvendo a relação entre representações sociais da alimentação, identidade e controle glicêmico não foram encontradas na literatura científica sobre o assunto. O objetivo desse estudo é analisar as representações sociais da alimentação das pessoas com diabetes mellitus tipo 2 (DM2) conforme representam a sua identidade e suas implicações no controle glicêmico. Participaram 34 usuários com DM2 de uma unidade básica de saúde de Belo Horizonte, Brasil. Utilizou-se a associação livre de palavras com justificativas de questões para identificar as representações sociais da identidade e da alimentação. As categorias das representações identitárias foram obtidas de um estudo anterior realizado com os mesmos participantes: aqueles que se consideram “normais”; os que aceitam a doença; os inconformados e os que levam a vida com dificuldades. As categorias das representações sociais da alimentação são: comer saudável, comer verduras e frutas, comer pouco, evitar doces, não comer de tudo, não comer muito e não seguir a dieta. É necessário desenvolver estudos que aumentem o conhecimento sobre as dificuldades e as necessidades das pessoas com diabetes e que promovam o seu envolvimento e o autocuidado.Qualitative research involving the relationship between social representations of diet, identity and glycemic control was not found in the scientific literature on the subject. The objective of this study is to analyze the social representations of the diet of people with type 2 diabetes mellitus (DM2) as they represent their identity and its implications for glycemic control. A total of 34 DM2 users from a basic health unit in Belo Horizonte, Brazil participated. We used the free association of words with justifications of questions to identify the social representations of identity and diet. The categories of identity representations were obtained from an earlier study conducted with the same participants: those who considered themselves "normal"; those who accept the disease; the nonconformists and those who lead a difficult life. The categories of social representations of diet are: eating healthy, eating vegetables and fruits, eating little, avoiding sweets, not eating at all, not eating too much and not following the diet. It is necessary to develop studies that increase awareness of the difficulties and needs of people with diabetes and promote their involvement and self-care.info:eu-repo/semantics/publishedVersio

Protective immunization against murine cytomegalovirus infection using adenoviruses and poxviruses expressing hepatitis B virus chimeras

Recombinant adenoviruses, poxviruses, and plasmid DNA vaccines encoding different hepatitis B virus (HBV)/murine cytomegalovirus (MCMV) protein chimeras were used to immunize mice. Processing of the chimeras resulted in presentation of a protective Ld/CD8+ T-cell epitope of the immediate early 1 protein pp89 (IE1 pp89) of MCMV to the immune system. Different levels of immunogenicity were observed depending on: (i) the type of viral vector used, (ii) whether the antigens were included in the cellular secretion pathway, and (iii) the location of the protective epitope within the chimeric protein. An adenovirus expressing a secretory HBV core protein with the MCMV epitope in its C-terminus induced the highest immune response. When the most immunogenic adenovirus and vaccinia virus were used in a heterologous prime-boost immunization protocol, even higher levels of epitope-specific T cells were obtained. Furthermore, responses were protective against a challenge with MCMV, inducing up to a 96% reduction of viral load in immunized animals, as determined by a sensitive real-time PCR assay. Together, these results confirmed previous observations of the efficient use of adenoviral and poxviral vectors in prime-boost protocols for immunization against diseases whose resolution depends on cellular immunity, as well as the aptness of correctly designed chimeric carrier proteins to facilitate this goal. [Int Microbiol 2007; 10(4):261-269