38 research outputs found

    STUDIES OF THE GENETIC EXPRESSION OF 31A-FIMBRIAE BY 2 BOVINE SEPTICEMIC ESCHERICHIA-COLI STRAINS

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    Using transposon tnphoA, classical bacterial genetic assays, SDS-PAGE and Western blot of superficial proteins, we have studied the expression of the 31A fimbriae of two bovine wild-type septicaemic Escherichia coli strains. The genes responsible for encoding colonization factor 31A were located in both the plasmid (strain BZ2468) and the chromosome (strain BZ43). The results obtained using HeLa cell cultures led us to believe that the BZ43 strain could have another colonization factor besides 31A, since one mutant, which did not express fimbriae, was still able to adhere to HeLa cells.17436537

    RELATIONSHIP AMONG ENTERO-TOXIGENIC PHENOTYPES, SEROTYPES, AND SOURCES OF STRAINS IN ENTERO-TOXIGENIC ESCHERICHIA-COLI

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    281242

    SINGLE RADIAL IMMUNE HEMOLYSIS TEST FOR DETECTION OF ESCHERICHIA-COLI THERMOLABILE ENTERO-TOXIN

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    14547347

    INCIDENCE OF PORCINE ROTAVIRUSES AT REGION OF CAMPINAS, SP, BRAZIL

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    19332733

    Genes coding for enterotoxins and verotoxins in porcine Escherichia coli strains belonging to different O:K:H serotypes: Relationship with toxic phenotypes

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    Seventy-four E. coli strains isolated from piglets with diarrhea or edema disease in Spain were serotyped and examined for production of heat-labile (LT) and heat-stable (ST) enterotoxins (LT-I, LT-II, STaH, STaP, and STb) and verotoxins (VT1, VT2, and VT2v VTe) by phenotypic (Vero cell assay and infant mouse test) and genotypic (colony hybridization and PCR) methods. In general, an excellent correlation was found between the results obtained with a PCR approach and those determined with biological assays. DNA probes used in the hybridization also showed a very good agreement with phenotypic results, with the exception of a VT1 probe that initially produced 10 false-positive reactions. The gene coding for STb (58 strains) was the most prevalent gene detected by PCR, followed by those coding for STa (46 strains), LT (19 strains), VT2v (11 strains), and VT1 (1 strain). Apparently, in Spain three seropathotypes are predominant: (i) 0149:K91:H10 K88(+) LT-I+ STb+ (ii) 0141:K85ab:H- P987(+) STaP+, and (iii) 0138:K81:H14 or H-STaP+ VT2v(+). We conclude that PCR is a fast, specific, and practical method for the identification of enterotoxin and VT genes in clinical and epidemiological studies.35112958296
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