Perfil eletrocardiográfico do coração de cobaia submetido ao extrato de Ginkgo biloba e seus terpenóides

Electrocardiographic effects produced by Ginkgo biloba extract (EGb) and by ginkgolides A (GA) and B (GB), and bilobalide (BB) were investigated in guinea pig heart mounted in Langendorff apparatus (Tyrode, 34 ± 0.1 oC, 95% O2, 5% CO2). Electrocardiographic parameters were evaluated in the conditions: 1) control with Tyrode and DMSO, 2) EGb (n=4), GA (n=5), GB (n=5) or BB (n=6), and 3) washout. The results showed that 0.1 and 1.0 mg/ml of EGb do not change the electrocardiographic parameters. However, 10 mg/ml of EGb increased the PR interval (PRi) at 21% (p<0.001). This increase was also observed for 50 mM GA (20%, p<0.001) and 70 mM BB (13%, p<0.001), which indicates Ca2+ channel block. However, the 50 mM GB reduced the PRi at 11 % (p<0.001). The GA (23%, p<0.001), GB (16%, p<0.001), and BB (40%, p<0.001) reduced the QT interval (QTi), which suggests the activation of the potassium channel. However, EGb increased QTi (6%, p<0.001). The EGb (28%, p<0.05) and GB (13%, p<0.05) reduced the heart rate. Atrioventricular (AV) block was observed with EGb, GA, and BB. We can conclude that EGb and its terpenoids alter the ECG parameters inducing AV block, which indicates possible arrhythmogenic potential._________________________________________________________________________________________ RESUMO: Os efeitos eletrocardiográficos produzidos pelo extrato de Ginkgo biloba (EGb) e gingkolídeos A (GA) e B (GB), e bilobalide (BB) foram investigados em coração de cobaia montado sistema de Langendorff (Tyrode, 34 ± 0.1 ºC, 95% O2, 5% CO2). Os parâmetros do ECG foram avaliados nas condições: 1) Tyrode e DMSO, 2) EGb (n=4), GA (n=5), GB (n=5) ou BB (n=6) diluídos em DMSO e 3) washout. Os resultados demonstram que 0,1 e 1,0 mg/mL de EGb não alteraram os parâmetros eletrocardiográficos. Entretanto, 10 mg/ml de EGb aumentaram o intervalo PR (PRi) em 21% (p<0.001). Esse aumento também foi observado com GA a 50µM (20%, p<0,001) e BB a 70 mM (13%, p<0,001) indicando bloqueio de canais de cálcio. Por outro lado, GB reduziu o PRi (11%, p<0,001). O intervalo QT (QTi) foi reduzido por GA (23%, p<0,001), GB (16%, p<0,001) e BB (40%, p < 0.001) sugerindo uma ativação de canais de potássio. Entretanto, EGb aumentou o QTi (6%, p<0.001). A frequência cardíaca foi reduzida por EGb (28%, p<0.05) e GB (13%, p<0.05). Bloqueios átrio-ventriculares (BAV) foram observados com EGb, GA e BB. Podemos concluir que EGb e os terpenos alteram parâmetros eletrocardiográficos induzindo BAV e demonstrando possível potencial arritmogênico

Therapeutic ultrasound associated with copaiba oil reduces pain and improves range of motion in patients with knee osteoarthritis

Abstract Introduction: Osteoarthritis is a disease that affects millions of Brazilians.Therapeutic ultrasound has been used in its treatment, either alone or associated with drugs. Objective: The aim of this study was to evaluate the effects of ultrasound (US) associated with Copaiba oil (CO) on knee osteoarthritis. Methods: Patients were divided into three different groups: US, US+CO, CO.Ten treatment sessions were held twice a week, 30 minutes each.Pain intensity was assessed through the Visual Analog Scale (VAS) and Range of Motion (ROM) by goniometry, and muscle strength was assessed by means of the Medical Research Council Scale. Statistical analysis was performed by Cohen’s d test, student’s t test and ANOVA, considering p<0.05 as significant. Results: Pain reduced in all groups.The US+CO group (d = -3.50) presented larger effect size when compared to the other groups. Regarding ROM, the largest effect size was observed in the US+CO group for flexion (d = 0.86) and extension (d = 0.97) in comparison with the remainder groups. Muscle strength increased in the US (d = 1.54) and US+CO (d = 1.60) groups for flexion.Regarding extension, the US group presented the largest effect size (d = 1.80). Conclusion: Therapeutic ultrasound associated with copaiba oil is a practical and effective therapy for the treatment of inflammatory diseases such as osteoarthritis

Avaliação do teste de 1600m sobre marcadores de estresse oxidativo e danos musculares em corredores juvenis

 Evaluation of the 1600m test on markers of oxidative stress and muscle damage in young runners The intense and prolonged physical exercise can cause oxidative stress and muscular damages, generating damage in the performance of the athlete. The aim of this study was to investigate the acute effect of the 1600m test on oxidative stress and muscle damage in young runners. Nine adolescents between 15 and 18 years of age participated in this study. The 1600m test was performed to evaluate the aerobic capacity, where the volunteers performed four laps on the 400m lane, making a total of 1600m. There was no increase in the tissue oxidative stress marker evaluated by the TBARS in the post-test. In relation to the antioxidant enzyme, the protocol did not promote an increase of glutathione activity. Plasma concentrations of lactate dehydrogenase and creatine kinase had a significant increase after the 1600m test compared to the pre-test. It was concluded that the 1600m test promotes muscle damage, however no oxidative stress was generated.O exercício físico intenso e prolongado pode ocasionar estresse oxidativo e danos musculares, gerando prejuízo no desempenho do atleta.  O estudo tem como objetivo investigar o efeito agudo do teste de 1600m sobre o estresse oxidativo e danos musculares em corredores juvenis. Participaram desse estudo nove adolescentes entre 15 e 18 anos de idade. Foi realizado o teste de 1600m para avaliar a capacidade aeróbia, onde os voluntários realizaram quatro voltas em pista de 400m, perfazendo um total de 1600m. Não houve aumento no marcador de estresse oxidativo tecidual avaliado pelo TBARS no pós-teste. Em relação à enzima antioxidante, o protocolo não promoveu um aumento da atividade da glutationa. Nas concentrações plasmáticas de lactato desidrogenase e da creatina quinase tiveram um aumento significativo depois do teste de 1600m comparado ao pré-teste. Conclui-se que o teste de 1600m promove danos musculares, no entanto não foi gerado estresse oxidativo