Fugitive emissions in Moravian-Silesian Region

Import 22/07/2015Predložená práca sa zaoberá fugitívnymi emisiami na území priemyselnej aglomerácie Ostravska. Fugitívny prach predstavuje hlavnú časť atmosférických aerosólov, zvýšená pozornosť je mu venovaná kvôli významným dopadom na zmenu klímy, kvalitu ovzdušia a zdravie ľudí a ekosystémov. Hlavná časť práce je venovaná štúdiu vertikálnej distribúcie PM1 vo výške až 500 m n. m., ktorá bola sledovaná vo vybraných lokalitách Ostravy v jarnom a letnom období 2014, za použitia metódy merania balónom. Pozornosť bola venovaná závislosti koncentrácie PM1 na výške a meteorologických podmienkach. Ďalej bolo zisťované rozloženie organických látok vo vertikálnych profiloch atmosféry v najzaťaženejších miestach Ostravy použitím metódy Py-GC/MS a pomocou matematických metód boli identifikované príspevky zdrojov znečistenia.This thesis deals with the topic of fugitive emissions in the industrial agglomeration of Ostrava region. Fugitive dust is a major part of atmospheric aerosols, increased attention is given to it due to its significant impact on climate change, air quality and human health, and ecosystems. The main part is focused on the study of the vertical distribution of PM1 of up to 500 m a. s. l. which was monitored at selected locations during spring and summer seasons of 2014 using the balloon measuring method. Attention was given to influence of meteorological parameters on PM1 concentrations. Furthermore, distribution of organic matter in the vertical profiles of the atmosphere in the most exposed places was studied using the Py-GC/MS and, using the mathematical methods, contributions of the sources of pollution were identified.Prezenční546 - Institut environmentálního inženýrstvívýborn

Origin of Structural Anomaly in Cuprous Halides

Cuprous halides (CuX; X = Cl, Br, or I) have been extensively investigated in the literature, but many of their fundamental properties are still not very well understood. For example, debate about their crystal stability, i.e., whether the ground-state structures of CuX are zinc-blende, still exists. By performing rigorous first-principles calculations for CuX using an accurate hybrid functional, we unambiguously demonstrate that CuX are indeed stable in the zinc-blende structure, but their accurate description requires careful treatment of the exchange interaction. Previous calculations based on local or semilocal density functionals underestimated the important contributions from exchange interactions and thus underestimated the energy separation between the unoccupied 4s and occupied 3d orbitals in Cu, resulting in an overestimation of the s–d coupling and the energy reduction of distorted CuX. Our study clarifies a long-standing and highly debated issue with regard to ground-state structures of CuX and advances the physics of phase stability and the importance of s–d coupling in semiconductors

Origin of Structural Anomaly in Cuprous Halides

Cuprous halides (CuX; X = Cl, Br, or I) have been extensively investigated in the literature, but many of their fundamental properties are still not very well understood. For example, debate about their crystal stability, i.e., whether the ground-state structures of CuX are zinc-blende, still exists. By performing rigorous first-principles calculations for CuX using an accurate hybrid functional, we unambiguously demonstrate that CuX are indeed stable in the zinc-blende structure, but their accurate description requires careful treatment of the exchange interaction. Previous calculations based on local or semilocal density functionals underestimated the important contributions from exchange interactions and thus underestimated the energy separation between the unoccupied 4s and occupied 3d orbitals in Cu, resulting in an overestimation of the s–d coupling and the energy reduction of distorted CuX. Our study clarifies a long-standing and highly debated issue with regard to ground-state structures of CuX and advances the physics of phase stability and the importance of s–d coupling in semiconductors

1N3R-tau induces S phase arrest in HEK293 cells.

<p><b>(A)</b> The distribution of HEK293 with six tau isoforms in sub G1 phase. <b>(B)</b> The distribution of HEK293 with six tau isoforms in G1 phase. *<i>P</i> < 0.05, compared with vector. <b>(C)</b> The distribution of HEK293 with six tau isoforms in S phase. **<i>P</i> < 0.01, compared with vector. <b>(D)</b> The distribution of HEK293 with six tau isoforms in G2/M phase. *<i>P</i> < 0.05, compared with vector.</p

1N3R-tau inhibits cell proliferation measured by BrdU incorporation.

<p>Fluorescent micrographs of eGFP and BrdU expression with six tau isoforms in N2a cells after 48 h transfection. GFP (Green), BrdU (Red). Quantification of BrdU-positive cells in eGFP expressing N2a cells with six tau isoforms after 48 h transfection. Scale bar = 50 μm. All values are standardized with vector. *<i>P</i> < 0.05, compared with vector.</p

Body weight and IPGTT in ApoE^−/−/LDLR^−/− mice.

<p>A: Body weight at the age of 3, 9, 11, 20, 22 and 24 weeks. B–D: Glucose tolerance tests at the age of 3, 9 and 11 weeks. E–G: The area under blood glucose concentration curve (AUC) at the age of 3, 9 and 11 weeks. Data are mean ± SEM; n = 16 per group. *<i>P</i><0.05 vs. Control.</p

1N3R tau induces translocation of cyclin E from nucleus to cytoplasm.

<p><b>(A)</b> N2a cells were transfected transiently with full length tau (2N4R), 1N3R tau and empty vector for 48 h, then cytoplasm and nuclear proteins were extracted and cyclin A, cyclin B1, cyclin D1, cyclin E, Cdk2, Cdk4 were detected by western blotting. DM1A and Lamin B1 represented loading and nuclear reference respectively. <b>(B)</b> Quantitative analysis of (A). All values are standardized with vector. *<i>P</i> < 0.05, compared with vector.</p

The effect of STAMP2 gene overexpression on inflammatory factors expression in WAT and BAT of ApoE^−/−/LDLR^−/− mice.

<p>A: Immunohistochemical staining for adipose tissue IL-6, TNF-α, MCP-1 and IL-10 (brown staining considered positive staining; scale bar: 50 µm). B: Semiquantification of IL-6, TNF-α, MCP-1 and IL-10 immunohistochemical staining. Data are mean ± SEM (three sections per mice; n = 4 per group). *<i>P</i><0.05 vs. Control+vehicle; ^#<i>P</i><0.05 vs. DM+vehicle. IOD: integrated optical density; EWAT: epididymal white adipose tissue; SWAT: subcutaneous white adipose tissue; BAT: brown adipose tissue.</p

Images and quantifications of STAMP2 expression in adipose tissues of ApoE^−/−/LDLR^−/− mice with or without STAMP2 overexpression.

<p>A: Immunohistochemical staining for adipose tissue STAMP2 (brown staining considered positive staining; scale bar: 50 µm); B. Representative Western blot of adipose tissue STAMP2; C. Western blot analysis of STAMP2 expression. Data are mean ± SEM; *<i>P</i><0.05 vs. Control+vehicle; ^#<i>P</i><0.05 vs. DM+vehicle. EWAT: epididymal white adipose tissue; SWAT: subcutaneous white adipose tissue; BAT: brown adipose tissue.</p