Early Childhood ABA: Issues of Early ASD Detection and Access to Intervention

This paper provides an overview of ABA, summarizes findings from studies of early childhood ABA with a focus on intervention dosage, and addresses the influence of demographics and culture on early ASD detection and access to early childhood ABA intervention. Although, this paper is not inclusive of all the ABA studies conducted between 1987-2007 and beyond, it summarizes the findings of some of the historical studies in early childhood ABA. These historical studies were located through two systematic reviews including studies between the years 1987- 2007 and selected if they met the following criteria: (1) intervention was based on behavioral principals (2) participants were five years of age or younger at the onset of intervention (3) intervention was in-home or clinic-based (4) utilized a group research design. A total of seven studies met the inclusion criteria. All seven studies reported increasing intellectual, educational, and behavioral gains for children with ASD. However, three of the studies did not report significant differences across all outcome measures favoring the treatment group. The seven studies reported mixed findings regarding treatment dosage with weekly treatment dosage ranging from 40 to 19.45 hours. Additional findings indicated that demographics and culture should be considered as they influence the early detection of ASD and access to intervention. Based on these findings, future research should further investigate how these variables along with others moderate or mediate early childhood ABA outcomes associated with early ASD detection and access to intervention

ADAPTATION OF MEDITERRANEAN CROPS TO WATER PRESSURE IN THE EBRO BASIN: A WATER EFFICIENCY INDEX

In this paper, we assess the output-oriented technical efficiency of agricultural production functions in order to compare, over time, economic and environmental production processes in the different regions of the Spanish Ebro basin, in a climate change context. The measurement of technical efficiency in agriculture can provide useful information about the competitiveness of farms and their potential to increase its productivity moreover can help in the crops adaptation to water pressure by improving the management of scarce resources. Here, we generate an agricultural water efficiency index to evaluate the adaptation of some Mediterranean crops to the water pressures in this area. We estimate frontier production functions and technical efficiency measures, using panel data models. This will allow us to observe changes in production due to individual specific effects and those that are time specific. To characterize our model, we use historical data, about crop yields, water requirements and climate as well as socio-economic and geographical aspects of the most representative crops in the provinces of the Ebro basin during 1976-2007. Then we generate a ranking of the most efficient crops across geographical areas, given their water use and other inputs, to evaluate policy scenarios with adjustments in water supply.water efficiency index in agriculture, Ebro basin, climate change adaptation, Crop Production/Industries, Resource /Energy Economics and Policy,

Selected Reaction Monitoring (SRM) frente a Western Blot

We propose the SRM technology as a complementary method to the Western Blot for the detection and quantification of proteins in a sample. The technique Western Blot has its own limitations: i) only a protein-of-choice is detected, ignoring any non-relevant proteins, ii) the sensitivity of the technique depends on the specificity of the antibody and iii) Western Blot is expensive and time-consuming. The advantages of SRM with respect Western Blot are remarkable: i) you can detect up to hundreds of different proteins in a sample, ii) SRM is more sensitive, because just 50 copies of the target protein per cell are enough for the detection and iii) once it has been made an investment in the necessary machinery to develop this technique, the detection of proteins in a sample turns into a cheaper, faster, more specific and full-quantitative procedure, without the need of using antibodies. First of all, SRM requires the identification of little peptides, obtained by tryptic digestion, whose sequence must be unique for a single protein or isoform. There is software for that aim. Then, it’s necessary to create isotope-labeled peptides of that identified for acting as internal standards. That sample is introduced in a triple quadrupole mass spectrometer: it passes through a first quadrupole, which functions as a filter, where the fragments are selected, previously ionized, attending to the mass/charge (m/z) relation that correspond to that unique fragments of the protein of interest. In this first selection may be other peptides from other proteins, with the same m/z but with different sequence. To select those that are exclusive from the target protein, the fragments are moved to a second quadrupole, where they are fragmented again with a physical method, and so new smaller fragments are generated. All the new fragments are conduced to the third quadrupole, where just those which come from the protein of interest are selected, attending at their m/z again. The target peptide concentration is determined by measuring the observed signal response for the target peptide relative to that of the isotopic-labeled peptide, the concentration of which is calculated from a pre-determined calibration-response curve. Calibration curves have to be generated for each target peptide in the sample. Because SRM technology is increasing its use, there have been developed databases where the scientific community upload information about protocols and standards for each protein with the aim to facilitate the work to other researchers