Severidade do oídio da videira em Juazeiro-BA.

Suplemento, ref. 272. Edição dos Resumos do XXXV Congresso Paulista de Fitopatologia, Jaguariúna, fev. 2012

Ocorrência do míldio da videira em diferentes épocas de podas em Petrolina - PE.

Suplemento. Edição dos Resumos do 44 Congresso Brasileiro de Fitopatologia, Bento Gonçalves, ago. 2011

Impacto de alterações de temperatura no crescimento e esporulação de Fusarium oxysporum f. sp. tracheiphilum.

A murcha-de-fusarium é uma severa doença do feijão-caupi. O desenvolvimento de estudos sobre este patossistema requer inoculações artificiais, sendo necessária a reprodução massal do inóculo. Sendo assim, o objetivo do trabalho foi determinar o comportamento de F. oxysporum f. sp. tracheiphilum quanto à produção de esporos e o crescimento vegetativo em diferentes temperaturas. Discos do micélio com 5 mm de diâmetro, foram transferidos para Placas de Petri contendo BDA, 15 , 20 , 25 , 30 e 35 ºC, sob fotoperíodo de 12 horas. Avaliou-se o diâmetro das colônias, durante 15 dias. Em câmara de Neubauer, quantificou-se o número de esporos produzidos em 1 mL de suspensão. A temperatura influenciou o crescimento micelial, sendo maior crescimento a 30º C. A maior produção de esporos ocorreu em 35º C. Verificou-se que a temperatura tem efeito no crescimento micelial e na produção de conídios de F. oxysporum f. sp. tracheiphilum, sendo que a faixa ótima, entre 30 e 35º C, otimizam o crescimento e a esporulação do fungo

Delayed enzymatic debridement in severe burns: Proof of concept

Introduction Enzymatic debridement (ED) is a novel powerful therapy for debridement of severe burns. Standard ED is usually performed within 72 h after injury following a presoaking phase. Little evidence exists on the effectiveness of ED later than 72 h after trauma. In this retrospective study, we compared outcomes of burn patients treated within versus later than 72 h after injury. Patients and Methods 110 patients with severe burns treated with ED between 2016 and 2020 were evaluated. Patients treated later than 72 h after trauma were identified and matched to a control group treated within 72 h. Matching criteria included age, area treated with ED, and localization of ED. Exclusion criteria were abbreviated burn severity index (ABSI) greater than 12 and death within the first 10 days after burn injury. Primary outcomes were time to full epithelialization and number of secondary surgical interventions. Results 16 patients (11 female, 5 male) matched the inclusion criteria and were assigned to the late treatment group. Mean age was 54.0 ± 19.0 years, the = and mean ABSI score 6.3 ± 3.2. 16 matched patients were assigned to the early ED group. Secondary surgical procedures were performed in 62.5% of cases in both groups with a mean of 1.7 (late treatment) vs. 2.2 (control; p = 0.29) secondary procedures in each group, respectively. No significant difference between groups regarding time to complete epithelialization (28.2 days vs. 27.3 days, p = 0.45) was observed. Infection rate was higher (18.8% vs. 6.3%, p = 0.28) in the delayed group. Conclusion Delayed ED is a feasible procedure as part of personalized care in burn surgery. In our retrospective study, we could not identify r safety issues except a slightly higher infection rate. This may however be attributed to delayed initiation of burn treatment itself

Novel compounds targeting the RNA-binding protein HuR : Structure-based design, synthesis and interaction studies

The key role of RNA-binding proteins (RBPs) in regulating post-transcriptional processes and their involvement in several pathologies (i.e., cancer and neurodegeneration) have highlighted their potential as therapeutic targets. In this scenario, Embryonic Lethal Abnormal Vision (ELAV) or Hu proteins and their complexes with target mRNAs have been gaining growing attention. Compounds able to modulate the complex stability could constitute an innovative pharmacological strategy for the treatment of numerous diseases. Nevertheless, medicinal-chemistry efforts aimed at developing such compounds are still at an early stage. As part of our ongoing research in this field, we hereby present the rational design and synthesis of structurally novel HuR ligands, potentially acting as HuR-RNA interferers. The following assessment of the structural features of their interaction with HuR, combining saturation-transfer difference NMR and in silico studies, provides a guide for further research on the development of new effective interfering compounds of the HuR-RNA complex

Exploration of ligand binding modes towards the identification of compounds targeting HuR : a combined STD-NMR and Molecular Modelling approach

Post-transcriptional processes have been recognised as pivotal in the control of gene expression, and impairments in RNA processing are reported in several pathologies (i.e., cancer and neurodegeneration). Focusing on RNA-binding proteins (RBPs), the involvement of Embryonic Lethal Abnormal Vision (ELAV) or Hu proteins and their complexes with target mRNAs in the aetiology of various dysfunctions, has suggested the great potential of compounds able to interfere with the complex stability as an innovative pharmacological strategy for the treatment of numerous diseases. Here, we present a rational follow-up investigation of the interaction between ELAV isoform HuR and structurally-related compounds (i.e., favonoids and coumarins), naturally decorated with diferent functional groups, by means of STD-NMR and Molecular Modelling. Our results represent the foundation for the development of potent and selective ligands able to interfere with ELAV–RNA complexes