8 research outputs found
Isolation of Viable Type I and II Methanotrophs Using Cell-Imprinted Polyurethane Thin Films
Studies
on methanotrophs utilizing methane as sole source of carbon
and energy are meaningful for governing global warming; although,
the isolation of methanotrophs from nature is challenging. Here, surface
imprinted polyurethane films were fabricated to selectively capture
living methanotrophs from paddy soil. Two tracks of molecularly imprinted
film based on polyurethane (PU-MIF<sub>1</sub> and PU-MIF<sub>2</sub>) were imprinted using type I or II methanotrophs as template, respectively,
and then reacted with polyethylene glycol, castor oil, and hexamethylene
diisocyanate. Results demonstrated these PU-MIFs hold low water absorption
rate and superior biocompatibility, which was highly demanded for
maintaining cell viability. Superior selectivity and affinity of PU-MIFs
toward their cognate methanotroph cells was observed by fluorescent
microscopy. Atomic force microscopy revealed the adhesion force of
PU-MIFs with its cognate cells was much stronger in comparison with
noncognate ones. Using the as-prepared PU-MIFs, within 30 min, methanotroph
cells could be separated from rice paddy efficiently. Therefore, the
PU-MIFs might be used as an efficient approach for cell sorting from
environmental samples
Acid-Catalyzed Synthesis of Trioxane in Aprotic Media
The effects of solvent, acid specificity,
acid concentration, added
salt, and reaction temperature on the yields of trioxane and formic
acid (byproduct) in paraformaldehyde + acid (or acid + salt) + aprotic
solvent were investigated. The mechanism that underlies the yield
of paraformaldehyde and the selectivity of trioxane was determined.
A highly practical and efficient synthesis of trioxane by a salt-mediated
and acid-catalyzed yield of paraformaldehyde in sulfolane media was
developed. The method increased the yield of paraformaldehyde by more
than 5 times and decreased the formic acid concentration by 10 times
compared to the commercial synthesis of trioxane in an aqueous reaction
system (formaldehyde + H<sub>2</sub>SO<sub>4</sub> + H<sub>2</sub>O)
Zwitterion-Immobilized Imprinted Polymers for Promoting the Crystallization of Proteins
Zwitterion additives have been used
in protein crystallization
to prevent the appearance of crystal clusters. Herein, we have developed
a novel approach for the immobilization of zwitterion onto molecularly
imprinted polymers (MIPs) to yield high-quality single protein crystals.
For lysozyme, trypsin, catalase, proteinase K, concanavalin A-type
IV, and thaumatin, simply adding the selected zwitterion (3-(methacryloylamino)propyl)-dimethyl(3-sulfopropyl)
ammonium hydroxide) into the free solution, the crystallization was
improved. When further using the zwitterion-immobilized molecularly
imprinted polymers (ziMIPs) developed in the current study, the formation
of higher quality crystals was facilitated in a shorter time compared
with regular MIPs and traditional crystallization trials. Most notably,
concanavalin A-type IV, which has nonunique ordered assembly, gave
only the form IV structure with higher resolution in the presence
of ziMIPs, justifying the superior function of ziMIPs for the ordered
assembly of protein molecules. Thus, the ziMIPs could be widely used
in protein crystallization
Additional file 1 of Insights into ZmWAKL in maize kernel development: genome-wide investigation and GA-mediated transcription
Additional file 1: Fig. S1
Additional file 2: of Genome-wide characterization of non-reference transposons in crops suggests non-random insertion
Supplemental Material. This file contains Tables S1âS3 and all the Figures S1âS7. (PDF 1647 kb
Cotula australis Hook. fil.
原著和名: マメカミツレ科名: キク科 = Compositae採集地: 東京都 浅草 三社 (武蔵 浅草 三社)採集日: 1980/11/24採集者: 萩庭丈壽整理番号: JH025392国立科学博物館整理番号: TNS-VS-97539
Self-Assembled TiO<sub>2</sub> Nanorods as Electron Extraction Layer for High-Performance Inverted Polymer Solar Cells
We
demonstrate the use of TiO<sub>2</sub> nanorods with well-controlled
lengths as excellent electron extraction materials for significantly
improving the performance of inverted polymer solar cells. The cells
containing long nanorods outperform the devices using amorphous TiO<sub>2</sub> particles as the electron extraction layer, mainly by a 2-fold
increase in short-circuit current and fill factor. The enhanced charge
extraction is attributed to the high electron mobility in crystalline
nanorods and their preferential alignment during film formation. Furthermore,
transient photocurrent studies suggest the presence of fewer interfacial
and internal defects in the nanorod interlayers, which can effectively
decrease carrier recombination and suppress electron trapping
Efficient Perovskite Solar Cells with Cesium Acetate-Modified TiO<sub>2</sub> Electron Transport Layer
The
photovoltaic performance of perovskite solar cells (PSCs) is
still below the Shockley–Queisser limit due to the impact of
defects originated from the surface and the bulk of the perovskite.
Hence, it is particularly important to alleviate non-radiative losses
in the solar cell by employing an interface modification strategy.
We implemented TiO2/CsAC as an electron transport layer
to achieve high-performance devices based on diethylammonium bromide
(DABr)-doped MAPbI3. The critical role of cesium acetate
(CsAC) is designed to improve perovskite crystallization and achieve
a high-quality interfacial contact between TiO2 and the
perovskite layer. TiO2/CsAC promotes the shift of Br ions
to form the Br-rich region at the perovskite/HTL interface simultaneously,
which can enhance the extraction of holes and block the diffusion
of electrons. Attributing to the modification of CsAC to TiO2, the performance of DABr-doped MAPbI3 PSC is improved
significantly