Isolation
of Viable Type I and II Methanotrophs Using
Cell-Imprinted Polyurethane Thin Films
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Abstract
Studies
on methanotrophs utilizing methane as sole source of carbon
and energy are meaningful for governing global warming; although,
the isolation of methanotrophs from nature is challenging. Here, surface
imprinted polyurethane films were fabricated to selectively capture
living methanotrophs from paddy soil. Two tracks of molecularly imprinted
film based on polyurethane (PU-MIF<sub>1</sub> and PU-MIF<sub>2</sub>) were imprinted using type I or II methanotrophs as template, respectively,
and then reacted with polyethylene glycol, castor oil, and hexamethylene
diisocyanate. Results demonstrated these PU-MIFs hold low water absorption
rate and superior biocompatibility, which was highly demanded for
maintaining cell viability. Superior selectivity and affinity of PU-MIFs
toward their cognate methanotroph cells was observed by fluorescent
microscopy. Atomic force microscopy revealed the adhesion force of
PU-MIFs with its cognate cells was much stronger in comparison with
noncognate ones. Using the as-prepared PU-MIFs, within 30 min, methanotroph
cells could be separated from rice paddy efficiently. Therefore, the
PU-MIFs might be used as an efficient approach for cell sorting from
environmental samples