“Nós fazíamos faísca um com o outro” : História de vida de um homicida

Dissertação de Mestrado apresentada ao ISPA - Instituto UniversitárioNo âmbito de um estudo qualitativo, o presente trabalho tem como base a história de vida de um sujeito do género masculino, de 30 anos de idade, que se encontra a cumprir pena por crime de homicídio num estabelecimento prisional da zona da grande Lisboa. O objectivo do trabalho prende-se com a compreensão dos significados e motivos que conduziram o sujeito à passagem ao acto, através da análise da sua trajectória de vida e do processo criminógeno. Para aceder à história de vida recorremos às entrevistas não estruturadas e semi-directivas, à função do próprio investigador e ao diário de campo. A história foi recolhida em contacto directo com o sujeito, tendo em atenção as suas vivências, experiências e acontecimentos importantes, dando espaço ao sujeito para se contar livremente. O método usado revelou-se eficaz pois permitiu aceder a uma compreensão do funcionamento psicológico do sujeito que, por sua vez, possibilitou entender como o acto homicida se inscreveu na vida do participante. A base da interpretação da história foi a teoria de Etienne De Greeff, através da análise de conteúdo e do biograma. Na discussão atendemos, também, à teoria psicanalítica e desenvolvimental, de modo a completar a compreensão da passagem ao acto. Compreendemos que o participante sofreu um processo criminógeno lento, marcado pela humilhação e por fenómenos de falsa compensação. As vivências traumáticas da infância, que conduziram a uma vinculação deficitária, a uma instabilidade emocional e ao insucesso na passagem pelo Édipo, apresentaram-se como facilitadores da passagem ao acto homicida.ABSTRACT: Within a qualitative study, this work is based on the life story of a male subject, aged 30, which was sentenced for the crime of murder and its now doing time in a prison in the Greater Lisbon area. The aim of the work is to understand the meanings and motives that led the subject to pass to the act, by examining his life path and his criminogen process. To access his life story we use the non-structured and semi-directives interviews, the role of the researcher and a field diary. The story was collected in direct contact with the subject, taking into account his experiences and major events, giving space to the subject to talk freely about himself. The method has proved effectiveness because it allowed to understand the psychological functioning of the subject which, in turn, allowed to understand how the homicidal act was part of the subject’s life. The interpretation of the history is based on the theory of Etienne De Greeff, through a content analysis and a biogram. In the discussion we also attended to the developmental and psychoanalytic theory in order to complete the understanding of the passage to the act. We understood that the participant has a slow criminogen process, marked by humiliation and the phenomena of false compensation. The traumatic experiences of childhood, which led to a week attachment, emotional instability and a failure in the passage by Oedipus, were facilitators of the transition to the homicidal act

[2]共同利用研究(平成9年度)

Additional file 3: Figure S3. Correlations between minimum feret diameter of gastrocnemius fibers from PAD subjects and walking performance (n=26, approximately 1000 fibers per subject). A) type I fiber minimum feret diameter versus normal-paced 4-m walking velocity; B) type I fiber minimum feret diameter versus fastest-paced 4-m walking velocity; C) average fiber minimum feret diameter versus normal-paced 4-m walking velocity; and D) average fiber minimum feret diameter versus fastest-paced 4-m walking velocity

Post-translational regulation of Necl-2 protein by TGF-β1 in GC-1 spg cells.

<p>(A) Necl-2 protein stability was analyzed by cycloheximide assay. Cells were pre-treated with CHX (5 µg/ml, 30 min) before vehicle or TGF-β1 treatment. Western blotting was performed to determine Necl-2 protein level. To determine the potential post-translational pathway, cells were pre-treated with CHX, followed by (B) proteasome inhibitor MG132 for 30 min, (C) caveolin inhibitor nystatin for 30 min or (D) clathrin inhibitor CPZ for 1 h or 1.5 h prior to TGF-β1 treatment (24 h). Necl-2 protein level was then analyzed by Western Blotting. (E) Immunofluorescence staining was performed in cells pretreated with CPZ followed by vehicle or TGF-β1 treatment. Cells were incubated with rabbit anti-Necl-2 antibody, followed by goat anti-rabbit IgG conjugated with FITC. Necl-2 appeared as green fluorescent. DAPI was used for nuclear staining. Scale bar  = 20 µm. A–D, Results are expressed as the mean±S.D. of three independent experiments. ns, not significant vs vehicle control; *, p<0.01 vs vehicle control; **, p<0.001 vs vehicle control. CHX, cycloheximide; CPZ, chlorpromazine.</p

Nucleotide sequence of primers used in RT-PCR, plasmid construction, EMSA, ChIP.

<p><i>Italic</i> base indicates mutated nucleotide. S, sense; AS, antisense. RT-PCR, reverse transcription-polymerase chain reaction; EMSA, electromobility shift assay; ChIP, chromatin immunoprecipitation.</p

Effect of TGF-β1 on Necl-2 mRNA stability and

<p><b>promoter activity.</b> (A) and (B) Analysis of Necl-2 mRNA stability was performed by actinomycin D (ActD) assay. Cells were pre-treated with ActD (5 µg/ml) for 2 h before vehicle or TGF-β1 treatment. RT-PCR (A) and real-time PCR (B) were performed to determine Necl-2 mRNA level. (C) Progressive 5′-deletion analysis of mouse Necl-2 promoter was performed between nt -502 and -1. A series 5′-deletion constructs and pEGFP vector were co-transfected into GC-1 spg cells followed by TGF-β1 treatment (5 ng/ml, 18 h). (D) Three putative <i>cis</i>-acting elements including MyoD, CCAATa and CCAATb motifs are located within the region between nt -159 and -1 (upper panel). Site-directed mutagenic constructs containing single, double or triple mutations and pEGFP vector were co-transfected into GC-1 spg cells followed by TGF-β1 treatment. pEGFP activity was used to normalize transfection efficiency. Promoter activity was represented as the fold change when compared with pGL-3 vector. (E) pGL-3 vector, p(-159/−1)Luc and pEGFP vector were co-transfected with si-Smad3 (#1/#2, 20 nM) or/and si-Smad4 (#1/#2, 20 nM) for 48 h followed by TGF-β1 treatment. pEGFP activity was used to normalize transfection efficiency. Smad3 and Smad4 protein levels were examined by Western blotting. (F) Wild-type and single mutated constructs of p(-159/−1)Luc were co-transfected with pcDNA3.1 vector, Smad3 or/and Smad4 expression constructs into GC-1 spg cells. The promoter activity was presented as a percentage of that of pcDNA3.1-transfected cells. (A–F), Results are expressed as the mean±S.D. of three independent experiments. ns, not significant vs vehicle control (A–E) or p(-159/−1)Luc (F); *, p<0.01 vehicle control (A–E) or vs p(-159/−1)Luc (F); **, p<0.001 vs vehicle control (A–E) or p(-159/−1)Luc (F). ActD, actinomycin D.</p

Effect of clathrin-shRNA on Necl-2 protein level and endocytosis assay.

<p>(A) Cells were transfected with sh-vector, sh-ctrl or sh-clathrin (#1 and #2) (1 µg) for 48 h, subsequently treated with CHX, followed by vehicle or TGF-β1 treatment. Level of clathrin, Necl-2 and actin were analysed by Western blotting. Actin was used as an internal control. Necl-2 protein levels were normalized with actin. (B) Immunofluorescence staining was performed in cells transfected with sh-vector, sh-ctrl or sh-clathrin followed by vehicle or TGF-β1 treatment. Cells were incubated with rabbit anti-Necl-2 antibody, followed by goat anti-rabbit IgG conjugated with Alexa Fluor 555. Necl-2 appeared as red fluorescent. DAPI was used for nuclear staining. Scale bar  = 20 µm. (C) GC-1 spg cells were biotinylated for 30 min. Cells were then incubated with either vehicle or TGF-β1 at 35°C to allow endocytosis, and reactions were terminated at various time points. Total = total labeled cell surface protein without stripping. Cell surface protein without biotinylation served as the negative control. Biotinylated proteins were pulled down by UltraLink Immobilized NeutrAvidin Plus beads and were subjected to Western blotting. Equal amounts of proteins were used at each time points as assessed by Actin blot. Percentage of internalized and biotinylated proteins remaining in the cytosol in the presence of vehicle or TGF-β1 are shown. The percentage of internalized and biotinylated Necl-2 at time 0 was arbitrarily set at 100. Results are expressed as the mean ± S.D. of three independent experiments. ns, not significant vs corresponding vehicle control; **, p<0.001 vs corresponding vehicle control. CHX, cycloheximide; Veh, vehicle control.</p

siRNAs and shRNAs used for different knockdown experiments in this study.

<p>siRNAs and shRNAs used for different knockdown experiments in this study.</p

Table_1_The Chinese customers and service staff interactive affective system (CCSIAS): introduction to a multimodal stimulus dataset.XLSX

To research the emotional interaction between customers and service staff, single-modal stimuli are being used to activate subjects’ emotions while multimodal emotion stimuli with better efficiency are often neglected. This study aims to construct a multimodal emotion stimuli database (CCSIAS) with video records of real work status of 29 service staff and audio clips of interactions between customers and service staff by setting up wide-angle cameras and searching in company’s Ocean Engine for 15 consecutive days. First, we developed a tool to assess the emotional statuses of customers and service staff in Study 1. Second, 40 Masters and PhD students were invited to assess the audio and video data to evaluate the emotional states of customers and service staff in Study 2, using the tools developed in Study 1. Third, 118 participants were recruited to test the results from Study 2 to ensure the stability of the derived data. The results showed that 139 sets of stable emotional audio & video data were constructed (26 sets were high, 59 sets were medium and 54 sets were low). The amount of emotional information is important for the effective activation of participants’ emotional states, and the degree of emotional activation of video data is significantly higher than that of the audio data. Overall, it was shown that the study of emotional interaction phenomena requires a multimodal dataset. The CCSIAS (https://osf.io/muc86/) can extend the depth and breadth of emotional interaction research and can be applied to different emotional states between customers and service staff activation in the fields of organizational behavior and psychology.</p

Data_Sheet_1_Effectiveness of different exercises in improving postural balance among Parkinson's disease patients: a systematic review and network meta-analysis.doc

BackgroundExercise has been reported as an effective intervention for Parkinson's disease. However, there is still debate on the what kinds of exercises prior to choosing. This study aimed to compare and rank the different exercises that effectively enhance postural balance in Parkinson's disease patients by quantifying the information gleaned from randomized controlled trials (RCTs).MethodsWe conducted a comprehensive database search, including PubMed, Cochrane Library, Embase, Web of Science, and PsycINFO. The included studies were evaluated for methodological quality by the Cochrane Risk of Bias tool.ResultsThe RCTs were collected between the earliest available date and March 2023. Sixty RCTs were included and the total sample size used in the study was 3,537. Thirty-five studies were defined as low risk of bias, twenty-one studies as medium risk of bias, and four studies as high risk of bias. The network meta-analysis results showed that exergaming exercise can significantly improve patients' Timed-Up-and-Go time (SUCRA = 91.5%). Dance can significantly enhance patients' Berg Balance Scale (surface under the cumulative ranking curve, SUCRA = 81.3%), and rhythmical auditory exercise can significantly improve patients' Mini-Balance Evaluation Systems Test score (SUCRA = 95.6%).ConclusionCompared with other exercises, exergaming exercise, Dance, and rhythmical auditory exercise showed superior efficacy in improving postural balance among Parkinson's disease patients.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier: CRD42023411918.</p

Forest plots for subgroup analysis of the association between rosacea and the prevalence of HP infection according to the strategies for determination of HP infection.

Forest plots for subgroup analysis of the association between rosacea and the prevalence of HP infection according to the strategies for determination of HP infection.</p