8 research outputs found
Blockchain-Based Privacy-Preserving System for Genomic Data Management Using Local Differential Privacy
The advances made in genome technology have resulted in significant amounts of genomic data being generated at an increasing speed. As genomic data contain various privacy-sensitive information, security schemes that protect confidentiality and control access are essential. Many security techniques have been proposed to safeguard healthcare data. However, these techniques are inadequate for genomic data management because of their large size. Additionally, privacy problems due to the sharing of gene data are yet to be addressed. In this study, we propose a secure genomic data management system using blockchain and local differential privacy (LDP). The proposed system employs two types of storage: private storage for internal staff and semi-private storage for external users. In private storage, because encrypted gene data are stored, only internal employees can access the data. Meanwhile, in semi-private storage, gene data are irreversibly modified by LDP. Through LDP, different noises are added to each section of the genomic data. Therefore, even though the third party uses or exposes the shared data, the owner’s privacy is guaranteed. Furthermore, the access control for each storage is ensured by the blockchain, and the gene owner can trace the usage and sharing status using a decentralized application in a mobile device
Inactivating transcription factor OsWRKY5 enhances drought tolerance through abscisic acid signaling pathways
During crop cultivation, water-deficit conditions retard growth, thus reducing crop productivity. Therefore, uncovering the mechanisms behind drought tolerance is a critical task for crop improvement. Here, we show that the rice (Oryza sativa) WRKY transcription factor OsWRKY5 negatively regulates drought tolerance. We determined that OsWRKY5 was mainly expressed in developing leaves at the seedling and heading stages, and that its expression was reduced by drought stress and by treatment with NaCl, mannitol, and abscisic acid (ABA). Notably, the genome-edited loss-of-function alleles oswrky5-2 and oswrky5-3 conferred enhanced drought tolerance, measured as plant growth under water-deficit conditions. Conversely, the overexpression of OsWRKY5 in the activation-tagged line oswrky5-D resulted in higher susceptibility under the same conditions. The loss of OsWRKY5 activity increased sensitivity to ABA, thus promoting ABA-dependent stomatal closure. Transcriptome deep sequencing and reverse transcription quantitative polymerase chain reaction analyses demonstrated that the expression of abiotic stress-related genes including rice MYB2 (OsMYB2) was upregulated in oswrky5 knockout mutants and downregulated in oswrky5-D mutants. Moreover, dual-luciferase, yeast one-hybrid, and chromatin immunoprecipitation assays showed that OsWRKY5 directly binds to the W-box sequences in the promoter region of OsMYB2 and represses OsMYB2 expression, thus downregulating genes downstream of OsMYB2 in the ABA signaling pathways. Our results demonstrate that OsWRKY5 functions as a negative regulator of ABA-induced drought stress tolerance, strongly suggesting that inactivation of OsWRKY5 or manipulation of key OsWRKY5 targets could be useful to improve drought tolerance in rice cultivars.Y
The Rice CHD3/Mi-2 Chromatin Remodeling Factor Rolled Fine Striped Promotes Flowering Independent of Photoperiod
Genetic studies have revealed that chromatin modifications affect flowering time, but the underlying mechanisms by which chromatin remodeling factors alter flowering remain largely unknown in rice (Oryza sativa). Here, we show that Rolled Fine Striped (RFS), a chromodomain helicase DNA-binding 3 (CHD3)/Mi-2 subfamily ATP-dependent chromatin remodeling factor, promotes flowering in rice. Diurnal expression of RFS peaked at night under short-day (SD) conditions and at dawn under long-day (LD) conditions. The rfs-1 and rfs-2 mutants (derived from different genetic backgrounds) displayed a late-flowering phenotype under SD and LD conditions. Reverse transcription-quantitative PCR analysis revealed that among the flowering time-related genes, the expression of the major floral repressor Grain number and heading date 7 (Ghd7) was mainly upregulated in rfs mutants, resulting in downregulation of its downstream floral inducers, including Early heading date 1 (Ehd1), Heading date 3a (Hd3a), and Rice FLOWERING LOCUS T 1 (RFT1). The rfs mutation had pleiotropic negative effects on rice grain yield and yield components, such as plant height and fertility. Taking these observations together, we propose that RFS participates in multiple aspects of rice development, including the promotion of flowering independent of photoperiod
Simple Electrodeposition of Dendritic Pd Without Supporting Electrolyte and Its Electrocatalytic Activity Toward Oxygen Reduction and H2O2 Sensing
Metallic palladium (Pd) electrocatalysts for oxygen reduction and hydrogen peroxide (H2O2) oxidation/reduction are prepared via electroplating on a gold metal substrate from dilute (5 to 50mM) aqueous K2PdCl4 solution. The best Pd catalyst layer possessing dendritic nanostructures is formed on the Au substrate surface from 50mM Pd precursor solution (denoted as Pd-50) without any additional salt, acid or Pd templating chemical species. The Pd-50consisted of nanostructured dendrites of polycrystalline Pd metal and micropores within the dendrites which provide high catalyst surface area and further facilitate reactant mass transport to the catalyst surface. The electrocatalytic activity of Pd-50 proved to be better than that of a commercial Pt (Pt/C) in terms of lower overpotential for the onset and half-wave potentials and a greater number of electrons (n) transferred. Furthermore, amperometric i-t curves of Pd-50 for H2O2 electrochemical reaction show high sensitivities (822.2 and -851.9μAmM-1cm-2) and low detection limits (1.1 and 7.91μM) based on H2O2 oxidation H2O2 reduction, respectively, along with a fast response (<1s). © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Mutation of ONAC096 Enhances Grain Yield by Increasing Panicle Number and Delaying Leaf Senescence during Grain Filling in Rice
5-Bromo-4′,5′-bis(dimethylamino)fluorescein: Synthesis and Photophysical Studies
In this study, three new fluorescein derivatives—5-bromo-4′,5′-dinitrofluorescein (BDNF), 5-bromo-4′,5′-diaminofluorescein (BDAF), and 5-bromo-4′,5′-bis(dimethylamino)fluorescein (BBDMAF)—were synthesized and their pH-dependent protolytic equilibria were investigated. In particular, BBDMAF exhibited pH-dependent fluorescence, showing strong emission only at pH 3–6. BBDMAF bears a bromine moiety and thus, can be used in various cross-coupling reactions to prepare derivatives and take advantage of its unique emission properties. To confirm this, the Suzuki and Sonogashira reactions of BBDMAF with phenylboronic acid and phenylacetylene, respectively, were performed, and the desired products were successfully obtained