Role of nicotine in depression through dopaminergic mechanism

Background: There are interesting reports in the literature indicating relationship of smoking /nicotine and depression. Smokers use nicotine to treat depression. The objectives of present study were to evaluate the role of nicotine in depression through Dopaminergic mechanism by using haloperidol induced catalepsy model in rats and to estimate Dopamine level in brain of depressed rats after nicotine and imipramine.Methods: Dopaminergic activity was evaluated in haloperidol induced catalepsy in rats. Levels of dopamine in normal as well as in depressed rats brain was estimated using fluorimetric method. The study treatment were administered as follows - Vehicle (s.c.), Imipramine (i.p.) - 7days, Nicotine (subcutaneous), Nicotine (inhalation) were administered in a dose of 1ml/kg,10mg/kg,0.4mg/kg,0.2mg/kg respectively.Results: In haloperidol induced catalepsy model, vehicle treated group showed cataleptic effect starting at 1 hour and lasting for 6 hours. Nicotine administered by subcutaneous route significantly reduced cataleptic score as compared to vehicle treated group till 6 hours. Nicotine administered by inhalation route reduced cataleptic score up to 6 hours compared with that of vehicle. Catalepsy score in nicotine (inhalation) group was significantly less as compared to nicotine (subcutaneous) at all time points period except 2 hours. Isolation induced hyperactivity model was used to induce depression in rats. Dopamine levels in rats after isolation were significantly less as compared to normal rats (before isolation). After isolation, dopamine levels in imipramine treated rats were significantly higher as compared to vehicle treated group. After isolation, dopamine levels were significantly high in both groups i.e., nicotine (subcutaneous) and nicotine (inhalation). Imipramine (7 days) and single dose of nicotine (inhalation) showed comparable results with normal dopamine level i.e. before isolation rats.Conclusions: Nicotine has increased dopaminergic activity as evident by reversal of haloperidol induced catalepsy. Dopamine level reduced in depressed rats. Dopamine brain levels were increased, when depressed rats were treated with Imipramine (i.p.), nicotine (s.c.), nicotine(inhaled). Single dose nicotine given by inhalation route has produced significant antidepressant action comparable to that of seven days’ treatment of standard antidepressant drug imipramine in rats. In rats, nicotine by both routes i.e subcutaneous and inhalational increased dopaminergic activity

Pharmacological Overview for Therapy of Gout and Hyperuricemia

Hyperuricemia & gout are disease conditions marked by over production and reduced excretion of uric acid. These conditions are linked with unhealthy lifestyle, Hypertension, Diabetes Mellitus, Metabolic syndrome, Cardiovascular & Chronic renal disease. Thus controlling & monitoring uric acid level becomes important. Development in the technology have led to greater insights into the pathophysiology of gout & hyperuricemia. Now we have a better understanding of involvement of interleukin 1β in inflammatory process of gout. Thus with better understanding newer therapeutic targets are being explored for treatment of gout & hyperuricemia. The armamentarium of drugs being used in therapy of acute gout here been expanded with recent addition by interleukin-1 inhibitors especially for refractory patients and patients with comorbidities.  As these new therapies are evolving we need to focus on improving the use of Allopurinol through patient education and training of physicians in order to minimize development of Allopurinol hypersensitivity syndrome (AHS). Further pretesting of Human leukocyte Antigen- B ( HLA-B*5801*) should be considered in Asian population. Febuxostat being critically new drug needs cautious approach, proper education of patients and Adverse Drug Reaction (ADR) reporting. With entry of Pegloticase there is a new class of drug added for treating hyperuricemia.&nbsp

Response of Combined Application of Nutrient Levels with Microbial Strains on Crop Growth, Nodulation and Yield of Soybean

The study focused to investigate the ‘Response of combined application of nutrient levels with microbial strains on crop growth, nodulation and yield of soybean (Glycine max (L.) Merrill.)’ at All India Coordinated Research Project (AICRP) on Integrated Farming System Research at College of Agriculture, Indore, Madhya Pradesh, India during kharif, 2019-20. The research was conducted in randomized block design (RBD) with 8 treatments, viz. Control with 75% RDF (T1), Control with 100% RDF (T2), 75% RDF with Bacillus megaterium and Bacillus mucilaginosus  strains as seed treatment (T3), 100% RDF with Bacillus megaterium and Bacillus mucilaginosus  strains as seed treatment (T4), 75% RDF with Bacillus megaterium and Bacillus mucilaginosus  strains as foliar application at 30 and 45 DAS (T5), 100% RDF with Bacillus megaterium and Bacillus mucilaginosus  strains as foliar application at 30 and 45 DAS (T6), 75% RDF with Bacillus megaterium and Bacillus mucilaginosus  strains as seed treatment + foliar application of Bacillus megaterium and Bacillus mucilaginosus  strains at 30 and 45 DAS (T7) and 100% RDF with Bacillus megaterium and Bacillus mucilaginosus  strains as seed treatment + foliar application of Bacillus megaterium and Bacillus mucilaginosus  strains at 30 and 45 DAS (T8). The seed yield of soybean increased to the tune of 14.26 and 19.72 per cent with application of 100% RDF with Bacillus megaterium and Bacillus mucilaginosus strains as seed treatment + foliar application of Bacillus megaterium and Bacillus mucilaginosus strains at 30 and 45 DAS compared to control. Among all the treatments, crop growth and yield (1139 kg/ha) were observed highest with 100% RDF with Bacillus megaterium and Bacillus mucilaginosus strains as seed treatment + foliar application of Bacillus megaterium and Bacillus mucilaginosus strains at 30 and 45 DAS

Evaluation of Wheat Varieties for Terminal Heat Stress under Varying Environments

Climate change and global warming have increasingly become relevant factors in recent years in determining the success of wheat production under heat stress conditions. Throughout its growth period wheat crop requires varying degrees of temperature to achieve ideal growth. Any variation from optimal temperature adversely affects plant growth and development. Many places where wheat is grown have high temperatures at the time of grain filling which is a major constraint on yield potential. A field experiment was conducted during the Rabi season of 2020-21 at Student’s Instructional Farm of Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Uttar Pradesh to evaluate the performance of wheat varieties under heat stress conditions. The treatment for the experiment consisted of sowing on three different dates i.e. D1 (30th November), D2 (15th December), D3 (30th December). It was observed that time of sowing decreased substantially almost all the yield components measured viz; number of ear bearing tillers per plant, number of grains per ear, ear length (cm), grain yield per plant (g), biological yield per plant (g), which caused severe reduction of yield in V1 (PBW-343) and V2 (HD-2967). This reduction was caused due to onset of high temperature during crop growth and particularly grain filling. Variety V3 (Halna) reduced the detrimental effect of heat stress by improving physiological traits which ultimately helped in obtaining higher yield

Not Available

Not AvailableIn this study, we evaluated the efcacy of Bacillus strains for plant growth promotional activities under in vitro and in vivo conditions. The results indicated that Bacillus megaterium BS11 was superior in enhancing the plant growth and yield of rice plants compared to other Bacillus strains. Currently, there is no information available on the molecular mechanism of Rice–B. megaterium interaction for plant growth promotion. Thus, the present study was undertaken to understand the molecular basis of Rice–B. megaterium interaction at the proteome level using the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) approach. Proteomic results revealed that a total of 17 proteins were diferentially expressed in response to BS11 inoculation. The application of BS11 up-regulated most of the identifed proteins involved in plant metabolism, transcription, transporter, signaling, defense, and stress responses, which may underlie the improvement of rice plant growth. Furthermore, the proteomic results were validated at the mRNA level by Quantitative Real-Time PCR (qPCR) analysis. The abundances of proteins and transcripts were positively correlated for all genes except LOC_Os01g68620. Overall, our results suggest that B. megaterium strain BS11 may promote plant growth by improving various metabolism in rice plants.Not Availabl