Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae.

To study the functions of heterogeneous nuclear ribonucleoproteins (hnRNPs), we have characterized nuclear polyadenylated RNA-binding (Nab) proteins from Saccharomyces cerevisiae. Nab1p, Nab2p, and Nab3p were isolated by a method which uses UV light to cross-link proteins directly bound to poly(A)+ RNA in vivo. We have previously characterized Nab2p, and demonstrated that it is structurally related to human hnRNPs. Here we report that Nab1p is identical to the Np13p/Nop3p protein recently implicated in both nucleocytoplasmic protein shuttling and pre-rRNA processing, and characterize a new nuclear polyadenylated RNA-binding protein, Nab3p. The intranuclear distributions of the Nab proteins were analyzed by three-dimensional immunofluorescence optical microscopy. All three Nab proteins are predominantly localized within the nucleoplasm in a pattern similar to the distribution of hnRNPs in human cells. The NAB3 gene is essential for cell viability and encodes an acidic ribonucleoprotein. Loss of Nab3p by growth of a GAL::nab3 mutant strain in glucose results in a decrease in the amount of mature ACT1, CYH2, and TPI1 mRNAs, a concomitant accumulation of unspliced ACT1 pre-mRNA, and an increase in the ratio of unspliced CYH2 pre-mRNA to mRNA. These results suggest that the Nab proteins may be required for packaging pre-mRNAs into ribonucleoprotein structures amenable to efficient nuclear RNA processing

A systematic review: Long range correlations in running gait

Long range correlations (LRCs) are apparent in human walking and running gait and are indicative of a healthy system. A systematic review (SR) was completed to determine the typical LRC patterns in running. 20 articles were included in the SR. LRCs in running gait tend to be similar to the LRCs in walking gait, with fatigue contributing to a negative effect on the LRCs. This SR could have implications on training guidelines for coaches and physical therapy methods to increase the LRCs in running gait for healthier functioning. More research is needed to determine how LRCs depend on running surface and what effect disease has on LRCs of running gait

Finding Forensic Evidence In the Operating System\u27s Graphical User Interface

A branch of cyber security known as memory forensics focuses on extracting meaningful evidence from system memory. This analysis is often referred to as volatile memory analysis, and is generally performed on memory captures acquired from target systems. Inside of a memory capture is the complete state of a system under investigation, including the contents of currently running as well as previously executed applications. Analysis of this data can reveal a significant amount of activity that occurred on a system since the last reboot. For this research, the Windows operating system is targeted. In particular, the graphical user interface component that includes the taskbar, start menu and notification system will be examined for possible forensic artifacts. The techniques presented in this research are valuable to a forensic investigator trying to find evidence. They are also useful for penetration testers trying to determine if a tool has left any evidence behind for investigators to find. The research described in this thesis led to development of a scanning technique that served as the basis for a Volatility plugin that automates finding GUI related artifacts. To support this research, a lab consisting of three virtual machines (VM) was created using VMware. Two Windows 10 virtual machines were created for generating artifacts and one Linux was created for scanning the Windows machines. These machines were connected to a live router briefly for gathering network information. This these explores the strengths and limitations of this searching discovered during research. Lastly, future applications of this research are covered

Increasing turbidity in the North Sea during the 20th century due to changing wave climate

Data on Secchi disc depth (the depth at which a standard white disc lowered into the water just becomes invisible to a surface observer) show that water clarity in the North Sea declined during the 20th century, with likely consequences for marine primary production. However, the causes of this trend remain unknown. Here we analyse the hypothesis that changes in the North Sea's wave climate were largely responsible by causing an increase in the concentrations of suspended particulate matter (SPM) in the water column through the resuspension of seabed sediments. First, we analysed the broad-scale statistical relationships between SPM and bed shear stress due to waves and tides. We used hindcasts of wave and current data to construct a space–time dataset of bed shear stress between 1997 and 2017 across the northwest European Continental Shelf and compared the results with satellite-derived SPM concentrations. Bed shear stress was found to drive most of the inter-annual variation in SPM in the hydrographically mixed waters of the central and southern North Sea. We then used a long-term wave reanalysis to construct a time series of bed shear stress from 1900 to 2010. This shows that bed shear stress increased significantly across much of the shelf during this period, with increases of over 20 % in the southeastern North Sea. An increase in bed shear stress of this magnitude would have resulted in a large reduction in water clarity. Wave-driven processes are rarely included in projections of climate change impacts on marine ecosystems, but our analysis indicates that this should be reconsidered for shelf sea regions

Novel results in STM, ARPES, HREELS, Nernst, neutron, Raman, and isotope substitution experiments and their relation to bosonic modes and charge inhomogeneity, from perspective of negative-Ueff boson-fermion modelling of HTSC

This paper seeks to synthesize much recent work on the HTSC materials around the latest STM results from Davis and coworkers. The conductance diffuse scattering results in particular are used as point of entry to discuss bosonic modes, both of condensed and uncondensed form. The bosonic mode picture is essential to understanding an ever growing range of observations within the HTSC field. The work is expounded within the context of the negative-U, boson-fermion modelling long advocated by the author. This general approach is presently seeing much theoretical development, into which I have looked to couple many of the experimental advances. While the formal theory is not yet sufficiently detailed to cover adequately all the experimental complexities presented by the real cuprate systems, it is clear that it affords very appreciable support to the line taken. An attempt is made throughout to say why and how it is that these events are tied so very closely to this particular set of materials.Comment: 36 pages pdf with 3 figures and 1 table included, Submitted to J. Phys. Cond. Mat

Ventilation with "clinically relevant" high tidal volumes does not promote stretch-induced injury in the lungs of healthy mice

OBJECTIVE: Ventilator-induced lung injury is a crucial determinant of the outcome of mechanically ventilated patients. Increasing numbers of mouse studies have identified numerous pathways and mediators that are modulated by ventilation, but it is conceptually difficult to reconcile these into a single paradigm. There is substantial variability in tidal volumes used in these studies, and no certainty about the pathophysiology that such varied models actually represent. This study was designed to investigate whether ventilation strategies ranging from ‘very high’ to more ‘clinically-relevant’ tidal volumes induce similar pathophysiologies in healthy mice, or represent distinct entities. DESIGN: In vivo study. SETTING: University research laboratory. SUBJECTS: C57/Bl6 mice. INTERVENTIONS: Anesthetised mice were ventilated with various tidal volumes up to 40ml/kg. MEASUREMENTS AND MAIN RESULTS: Respiratory system compliance and arterial blood gases were used to evaluate physiological parameters of injury. Lung wet:dry weight ratio, lavage fluid protein and cytokines were used to assess pulmonary edema and inflammation. All ventilation strategies induced changes in respiratory system compliance, although the pattern of change was unique for each strategy. 10ml/kg and 40ml/kg ventilation also induced decreases in arterial pO(2) and blood pressure. Any physiological changes induced during the 10, 20 and 30ml/kg strategies were largely reversed by recruitment maneuvers at the end of the protocol. Markers of pulmonary edema and inflammation indicated that only 40ml/kg induced substantial increases in both, consistent with development of lung injury. CONCLUSIONS: Tidal volumes up to 20ml/kg are unlikely to induce substantial lung over-stretch in models using healthy, young mice. Signs of injury/inflammation using such models are likely to result from other factors, particularly alveolar derecruitment and atelectasis. The results of such studies may need to be re-evaluated before clinical relevance can be accurately determined