The Zwicky Transient Facility: Surveys and Scheduler

We present a novel algorithm for scheduling the observations of time-domain imaging surveys. Our Integer Linear Programming approach optimizes an observing plan for an entire night by assigning targets to temporal blocks, enabling strict control of the number of exposures obtained per field and minimizing filter changes. A subsequent optimization step minimizes slew times between each observation. Our optimization metric self-consistently weights contributions from time-varying airmass, seeing, and sky brightness to maximize the transient discovery rate. We describe the implementation of this algorithm on the surveys of the Zwicky Transient Facility and present its on-sky performance.Comment: Published in PASP Focus Issue on the Zwicky Transient Facility (https://dx.doi.org/10.1088/1538-3873/ab0c2a). 13 Pages, 11 Figure

Mixed composition materials suitable for vacuum web sputter coating

Ion beam sputter deposition techniques were used to investigate simultaneous sputter etching of two component targets so as to produce mixed composition films. Although sputter deposition has been largely confined to metals and metal oxides, at least one polymeric material, poly-tetra-fluorethylene, has been demonstrated to produce sputtered fragments which repolymerize upon deposition to produce a highly cross-linked fluoropolymer resembling that of the parent target Fluoropolymer-filled silicon dioxide and fluoropolymer-filled aluminum oxide coatings have been deposited by means of ion beam sputter coat deposition resulting in films having material properties suitable for aerospace and commercial applications. The addition of fluoropolymer to silicon dioxide films was found to increase the hydrophobicity of the resulting mixed films; however, adding fluoropolymer to aluminum oxide films resulted in a reduction in hydrophobicity, thought to be caused by aluminum fluoride formation

Climate Change and Market-Based Insurance Feedbacks

Climatic events have accounted for 91% of $1.05 trillion in insured costs for global catastrophic events from 1980 to 2016. Costs are driven by socio-economic development and increased frequency and severity of climatic disasters driven by climate change. Government policies to reduce systemic risk (e.g., cap-and-trade, carbon tax) have been a predominant approach for mitigation and adaptation. Alternatively, market-based incentives for climate change adaptation and mitigation already operate via the insurance industry to lessen impacts on society. Insurance feedbacks include changes in 1) premiums and insurance policies, 2) non-coverage, and 3) policy making and litigation. Alongside government policies, insurance feedbacks could be used to facilitate climate change adaptation and mitigation to a significant degree. Ultimately, a negotiated distribution of climate-related costs between the public and private insurance is needed

Correlates of Bird Collisions With Buildings Across Three North American Countries

Collisions with buildings cause up to 1 billion bird fatalities annually in North America. Bird-building collisions have recently received increased conservation, research, and policy attention. However, efforts to reduce collisions would benefit from studies conducted at large spatial scales across multiple study sites, with standardized methods, and with consideration of species- and life history-related variation and correlates of collisions. We addressed these research needs with a coordinated data collection effort at 40 sites across North America. We estimated collision vulnerability for 40 bird species by accounting for their North American population abundance, distribution overlap with study sites, and sampling effort. Of 10 species we identified as most vulnerable to collisions, some have been identified in past studies (e.g., Black-throated Blue Warbler [Setophaga caerulescens]) while others emerged for the first time (e.g., White-breasted Nuthatch [Sitta carolinensis]), possibly because we used a more standardized sampling approach than past studies. Analyses of species-specific collision correlates revealed that building size and glass area were positively associated with numbers of collisions for 5 of 8 species with enough observations to analyze independently. Vegetation around buildings influenced collisions for only 1 of those 8 species (Swainson\u27s Thrush [Catharus fuscescens]). We also found that life history predicted collisions; numbers of collisions were greatest for migratory, insectivorous, and woodland-inhabiting species. This coordinated, continent-wide study provides new insight into the species most vulnerable to building collisions, making them potentially in greatest need of conservation attention to reduce collisions. This study also lends insight into species- and life history-related variation and correlates of building collisions, information that can help refine collision management efforts

Comparison of burbot populations across adjacent native and introduced ranges

Introduced species are a threat to biodiversity. Burbot, Lota lota, a fish native to the Wind River Drainage, Wyoming and a species of conservation concern, have been introduced into the nearby Green River Drainage, Wyoming, where they are having negative effects on native fish species. We compared these native and introduced burbot populations to evaluate potential mechanisms that could be leading to introduction success. We examined genetic ancestry, physical habitat characteristics, community composition, and burbot abundance, relative weight, and size structure between the native and introduced range to elucidate potential differences. The origin of introduced burbot in Flaming Gorge Reservoir is most likely Boysen Reservoir and several nearby river populations in the native Wind River Drainage. Burbot populations did not show consistent differences in abundance, size structure, and relative weight between drainages, though Fontenelle Reservoir, in the introduced drainage, had the largest burbot. There were also limited environmental and community composition differences, though reservoirs in the introduced drainage had lower species richness and a higher percentage of non-native fish species than the reservoir in the native drainage. Burbot introduction in the Green River Drainage is likely an example of reservoir construction creating habitat with suitable environmental conditions to allow a southwards range expansion of this cold-water species. An understanding of the factors driving introduction success can allow better management of species, both in their introduced and native range

(±)-2-exo- and endo-Methylamino-1,2,3,4-tetrahydro-1,4-ethanonapthalene Hydrochloride

This is the published version

Expression of NeuGc on Pig Corneas and Its Potential Significance in Pig Corneal Xenotransplantation

PURPOSE: Pigs expressing neither galactose-α1,3-galactose (Gal) nor N-glycolylneuraminic acid (NeuGc) take xenotransplantation one step closer to the clinic. Our aims were (1) to document the lack of NeuGc expression on corneas and aortas and cultured endothelial cells [aortic endothelial cells (AECs); corneal (CECs)] of GTKO/NeuGcKO pigs, and (2) to investigate whether the absence of NeuGc reduced human antibody binding to the tissues and cells. METHODS: Wild-type (WT), GTKO, and GTKO/NeuGcKO pigs were used for the study. Human tissues and cultured cells were negative controls. Immunofluorescence staining was performed using anti-Gal and anti-NeuGc antibodies, and human IgM and IgG binding to tissues was determined. Flow cytometric analysis was used to determine Gal and NeuGc expression on cultured CECs and AECs and to measure human IgM/IgG binding to these cells. RESULTS: Both Gal and NeuGc were detected on WT pig corneas and aortas. Although GTKO pigs expressed NeuGc, neither humans nor GTKO/NeuGcKO pigs expressed Gal or NeuGc. Human IgM/IgG binding to corneas and aortas from GTKO and GTKO/NeuGcKO pigs was reduced compared with binding to WT pigs. Human antibody binding to GTKO/NeuGcKO AECs was significantly less than that to GTKO AECs, but there was no significant difference in binding between GTKO and GTKO/NeuGcKO CECs. CONCLUSIONS: The absence of NeuGc on GTKO aortic tissue and AECs is associated with reduced human antibody binding, and possibly will provide a better outcome in clinical xenotransplantation using vascularized organs. For clinical corneal xenotransplantation, the absence of NeuGc expression on GTKO/NeuGcKO pig corneas may not prove an advantage over GTKO corneas

Synergistic Effects of Salinity and Temperature on the Survival of Two Nonnative Bivalve Molluscs, Perna viridis

This study examined the combined salinity and temperature tolerance of two marine bivalve molluscs, Perna viridis and Mytella charruana, which have recently invaded the southeastern United States. It is essential to understand the role that these abiotic variables play in invasions and establishment of nonnative species. We simultaneously explored survival at three salinity ranges (5–9, 20–22.5, and 35–40 ppt) in both cold and warm water for juveniles and adults of both species. We determined that Perna viridis can survive at a wide range of temperatures (9–35°C) when the salinity is 35–37 ppt; however, as salinity decreased, the thermal survival range for P. viridis became narrower. With M. charruana, our data suggest that juvenile and adult individuals can survive at a wide range of salinities (5–40 ppt) at 20°C, but the salinity tolerance range narrowed as the temperature decreased or increased. Additionally, we observed that temperature rapidly impacted survival of P. viridis and M. charruana (within hours), while salinity impacts were more gradual (days to weeks). These data can be used to help predict successful introductions and future expansions of P. viridis and M. charruana in introduced habitats

Zanamivir susceptibility monitoring and characterization of influenza virus clinical isolates obtained during phase II clinical efficacy studies

Zanamivir is a highly selective neuraminidase (NA) inhibitor with demonstrated clinical efficacy against influenza A and B virus infections. In phase II clinical efficacy trials (NAIB2005 and NAIB2008), virological substudies showed mean reductions in virus shedding after 24 h of treatment of 1.5 to 2.0 log(10) 50% tissue culture infective doses compared to a placebo, with no reemergence of virus after the completion of therapy. Paired isolates (n = 41) obtained before and during therapy with zanamivir demonstrated no shifts in susceptibility to zanamivir when measured by NA assays, although for a few isolates NA activity was too low to evaluate. In plaque reduction assays in MDCK cells, the susceptibility of isolates to zanamivir was extremely variable even at baseline and did not correlate with the speed of resolution of virus shedding. Isolates with apparent limited susceptibility to zanamivir by plaque reduction proved highly susceptible in vivo in the ferret model. Further sequence analysis of paired isolates revealed no changes in the hemagglutinin and NA genes in the majority of isolates. The few changes observed were all natural variants. No amino acid changes that had previously been identified in vitro as being involved with reduced susceptibility to zanamivir were observed. These studies highlighted problems associated with monitoring susceptibility to NA inhibitors in the clinic, in that no reliable cell-based assay is available. At present the NA assay is the best available predictor of susceptibility to NA inhibitors in vivo, as measured in the validated ferret model of infection