System approach to robust acoustic echo cancellation through semi-blind source separation based on independent component analysis

We live in a dynamic world full of noises and interferences. The conventional acoustic echo cancellation (AEC) framework based on the least mean square (LMS) algorithm by itself lacks the ability to handle many secondary signals that interfere with the adaptive filtering process, e.g., local speech and background noise. In this dissertation, we build a foundation for what we refer to as the system approach to signal enhancement as we focus on the AEC problem. We first propose the residual echo enhancement (REE) technique that utilizes the error recovery nonlinearity (ERN) to "enhances" the filter estimation error prior to the filter adaptation. The single-channel AEC problem can be viewed as a special case of semi-blind source separation (SBSS) where one of the source signals is partially known, i.e., the far-end microphone signal that generates the near-end acoustic echo. SBSS optimized via independent component analysis (ICA) leads to the system combination of the LMS algorithm with the ERN that allows for continuous and stable adaptation even during double talk. Second, we extend the system perspective to the decorrelation problem for AEC, where we show that the REE procedure can be applied effectively in a multi-channel AEC (MCAEC) setting to indirectly assist the recovery of lost AEC performance due to inter-channel correlation, known generally as the "non-uniqueness" problem. We develop a novel, computationally efficient technique of frequency-domain resampling (FDR) that effectively alleviates the non-uniqueness problem directly while introducing minimal distortion to signal quality and statistics. We also apply the system approach to the multi-delay filter (MDF) that suffers from the inter-block correlation problem. Finally, we generalize the MCAEC problem in the SBSS framework and discuss many issues related to the implementation of an SBSS system. We propose a constrained batch-online implementation of SBSS that stabilizes the convergence behavior even in the worst case scenario of a single far-end talker along with the non-uniqueness condition on the far-end mixing system. The proposed techniques are developed from a pragmatic standpoint, motivated by real-world problems in acoustic and audio signal processing. Generalization of the orthogonality principle to the system level of an AEC problem allows us to relate AEC to source separation that seeks to maximize the independence, hence implicitly the orthogonality, not only between the error signal and the far-end signal, but rather, among all signals involved. The system approach, for which the REE paradigm is just one realization, enables the encompassing of many traditional signal enhancement techniques in analytically consistent yet practically effective manner for solving the enhancement problem in a very noisy and disruptive acoustic mixing environment.PhDCommittee Chair: Biing-Hwang Juang; Committee Member: Brani Vidakovic; Committee Member: David V. Anderson; Committee Member: Jeff S. Shamma; Committee Member: Xiaoli M

A continental-scale hydro-economic model for integrating water-energy-land nexus solutions

This study presents the development of a new bottom‐up large‐scale hydro‐economic model, Extended Continental‐scale Hydro‐economic Optimization (ECHO), that works at a sub‐basin scale over a continent. The strength of ECHO stems from the integration of a detailed representation of local hydrological and technological constraints with regional and global policies, while accounting for the feedbacks between water, energy and agricultural sectors. In this study, ECHO has been applied over Africa as a case study with the aim of demonstrating the benefits of this integrated hydro‐economic modeling framework. Results of this framework are overall consistent with previous findings evaluating the cost of water supply and adaptation to global changes in Africa. Moreover, results provide critical assessments of future investment needs in both supply and demand side water management options, economic implications of contrasting future socio‐economic and climate change scenarios, and the potential tradeoffs among economic and environmental objectives. Overall, this study demonstrates the capacity of ECHO to address challenging research questions examining the sustainability of water supply, and the impacts of water management on energy and food sectors and vice versa. As such, we propose ECHO as useful tool for water‐related scenario analysis and management options evaluation

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Operando Observation of Ni2P Structural Changes during Catalytic Reaction: Effect of H2S Pretreatment

In situ X-ray absorption spectroscopy was used to study the effect of H2S pretreatment on the activation of Ni2P catalysts for the thiophene hydrodesulfurization (HDS) reaction. It was found that H2S treatment produced Ni-S bonds that were slightly longer and that had higher coordination numbers than those formed during reaction. The longer Ni-S bonds formed by H2S treatment were changed to the shorter Ni-S bonds by the catalytic reaction, and these shorter bonds were associated with high activity. The work provides evidence that a NiPS phase is active in the HDS reaction

Operando QEXAFS studies of Ni2P during thiophene hydrodesulfurization: direct observation of Ni-S bond formation under reaction conditions

Structural changes in Ni2P/MCM-41 were followed by quick extended X-ray absorption fine structure (QEXAFS) and were directly related to changes in X-ray absorption near-edge structure (XANES) which had been used earlier for the study of the active catalyst phase. An equation is proposed to correct the transient QEXAFS spectra up to second-order in time to remove spectral distortions induced by structural changes occurring during measurements. A good correlation between the corrected QEXAFS and the XANES spectral changes was found, giving support to the conclusions derived from the XANES in the previous work, namely that the formation of a Ni-S bond in a surface NiPS phase is involved in the active site for the hydrodesulfurization reaction