From regional climate simulations to the hydrological information needed for basin scale impact studies

The accuracy of local downscaling of rainfall predictions provided by climate models is crucial for the assessment of climate change impacts on hydrological processes because the presence of bias in downscaled precipitation may produce large bias in the assessment of soil moisture dynamics, river flows, and groundwater recharge. <br><br> In this study, the output of a regional climate model (RCM) is downscaled using a stochastic modelling of the point rainfall process able to adequately reproduce the daily rainfall intermittency which is one of the crucial aspects for the hydrological processes characterizing Mediterranean environments. The historical time-series from a dense rain-gauge network were used for the analysis of the RCM bias in terms of dry and wet daily period and then to investigate the predicted alteration in the local rainfall regime. A Poisson Rectangular Pulse (PRP) model (Rodriguez-Iturbe et al., 1987) was finally adopted for the stochastic generation of local daily rainfall as a continuous-time point process with forcing parameters resulting from the bias correction of the RCM scenario

Intrinsic vulnerability assessment of the south-eastern Murge (Apulia, southern Italy)

Maps of areas with different vulnerability degrees are an integral part of environmental protection and management policies. It is difficult to assess the intrinsic vulnerability of karst areas since the stage and type of karst structure development and its related underground discharge behaviour are not easy to determine. Therefore, some improvements, which take into account dolines, caves and superficial lineament arrangement, have been integrated into the SINTACS R5 method and applied to a karst area of the south-eastern Murge (Apulia, southern Italy). The proposed approach integrates the SINTACS model giving more weight to morphological and structural data; in particular the following parameters have been modified: depth to groundwater, effective infiltration action, unsaturated zone attenuation capacity and soil/overburden attenuation capacity. Effective hydrogeological and impacting situations are also arranged using superficial lineaments and karst density. In order to verify the reliability of the modified procedure, a comparison is made with the original SINTACS R5 index evaluated in the same area. The results of both SINTACS index maps are compared with karst and structural features identified in the area and with groundwater nitrate concentrations recorded in wells. The best fitting SINTACS map is then overlaid by the layout of potential pollution centres providing a complete map of the pollution risk in the area

Drinking water supply in resilient cities: Notes from L'Aquila earthquake case study

Disasters impacts on urban environment are the result of interactions among natural and human systems, which are intimately linked each other. What is more, cities are directly dependent on infrastructures providing essential services (Lifeline Systems, LS). The operation of LS in ordinary conditions as well as after disasters is crucial. Among the LS, drinking water supply deserves a critical role for citizens. The present work summarizes some preliminary activities related to an ongoing EU funded research project. The main aim of the paper is to define a System Dynamic Model (SDM) to assess the evolution of resilience of a drinking water supply system in case of natural disasters, with particular attention to the role of both ‘structural’ and ‘non-structural’ parameters. Reflections are carried out on L’Aquila (Italy) case study, since drinking water infrastructures were significantly stressed during the 2009 earthquake, causing a limited functionality in the aftermath of the event. Furthermore, the reallocation of citizens in temporary shelters determined a change in the demand pattern, requiring a dynamic adaptation of the infrastructure. Based on an innovative approach to resilience, the model was developed also to simulate different emergency management scenarios, corresponding to different disaster management strategies

Drazepinone, a trisubstituted tetrahydronaphthofuroazepinone with herbicidal activity produced by Drechslera siccans

When grown in a minimal-defined medium, a strain of Drechslera siccans, a pathogenic fungus isolated from seeds of Lolium perenne, produced phytotoxic metabolites. This strain is one of the best toxin producers among several grass pathogenic fungal strains collected and tested to find phytotoxins to be used as natural herbicides of monocot weeds. From the culture filtrates of D. siccans, we isolated a new phytotoxic trisubstituted naphthofuroazepinone, named drazepinone, and characterised it as a 3,5,12a-trimethyl-2,5,5a,12a-tetrahydro- 1H-naphtho[2′,3′:4,5]furo[2,3-b]azepin-2-one. Assayed at 2 μg μl-1 solution the novel metabolite proved to have broad-spectrum herbicidal properties, without antibacterial and antifungal activities, and low zootoxic activity. Its original chemical structure and the interesting biological properties make drazepinone a potential natural herbicide. © 2005 Elsevier Ltd. All rights reserved

Herbicidal potential of ophiobolins produced by Drechslera gigantea

Drechslera gigantea, a potential mycoherbicide of grass weeds, was isolated in Florida from naturally infected large crabgrass (Digitaria sanguinalis); it produces phytotoxic metabolites in liquid culture. The main metabolite was identified by spectroscopic methods and optical properties as ophiobolin A (1), a well-known phytotoxic sesterterpene produced by several phytopathogenic fungi of important crops and already extensively studied for its interesting biological activities. The other three minor metabolites proved to be related to ophiobolin A and were identified using the same techniques as 6-epi-ophiobolin A and 3-anhydro-6-epi-ophiobolin A (2 and 3) and ophiobolin I (4). Assayed on punctured detached leaves of several grass and dicotyledon weeds, ophiobolin A proved to be on average more phytotoxic as compared to the other related compounds. Some structural features appear to be important for the phytoxicity, such as the hydroxy group at C-3, the stereochemistry at C-6, and the aldehyde group at C-7. Furthermore, grass weeds usually proved to be more sensitive to the phytotoxins than dicotyledons, on which ophiobolin A caused the appearance of large necrosis even at the lowest concentration assayed. This is the first report about the production of ophiobolins from D. gigantea and of the proposed use as potential natural herbicides against grass weeds. © 2006 American Chemical Society

The physics of plasma membrane photostimulation

Cell membrane perturbation is a common way to stimulate cells by using external actuators. Recently, nanotechnology has added a number of new strategies for doing this, enlarging the scope and the range of mechanisms involved. Here, we describe a number of possible perturbation actions that are driven by light, and we try to capture the underlying phenomena. The discussion is based on the simple equivalent circuit model for the cell membrane

Ophiobolin E and 8-epi-ophiobolin J produced by Drechslera gigantea, a potential mycoherbicide of weedy grasses

Drechslera gigantea, a fungal pathogen isolated from large crabgrass (Digitaria sanguinalis) and proposed as a potential mycoherbicide of grass weeds, produces phytotoxic metabolites in liquid and solid cultures. Ophiobolin A and three minor ophiobolins i.e., 6-epi-ophiobolin A, 3-anhydro-6-epi-ophiobolin A and ophiobolin I were obtained from the liquid culture broths. Interestingly and unexpectedly, ophiobolins also appeared in cultures of this fungus and they were isolated together with the known ophiobolins B and J, and designed as ophiobolin E and 8-epi-ophiobolin J. They were characterized using essentially spectroscopic methods. It is noteworthy that D. gigantea produces such a plethora of bioactive organic substances. Some structure-activity relationship results are also discussed in this report. © 2006 Elsevier Ltd. All rights reserved

Evaluation of dittrichia viscosa (L.) greuter dried biomass for weed management

Dittrichia viscosa (L.) Greuter, a plant species common in the Mediterranean basin, produces several bioactive compounds, some of which have herbicidal effects. A number of greenhouse and field experiments were carried out in order to evaluate if these effects could be obtained also by using the whole plant biomass, to identify the efficacious doses, determine their effects on seed germination and weed emergence, and to evaluate influence of soil characteristics on biomass efficacy. The experiments carried out evidenced that: (i) the dried biomass completely hampers plant emergence when high doses (30–40 kg biomass m−3 of soil) are mixed into the soil, or delays it at a lower dose (10 kg m−3 ); (ii) the detrimental effects are not affected by soil type. The exploitation of the D. viscosa dried biomass appears to be a feasible option in weed management practices and its potential is discussed

Detection of Listeria monocytogenes in foods with a textile organic electrochemical transistor biosensor

Abstract: Foods contaminated by pathogens are responsible for foodborne diseases which have socioeconomic impacts. Many approaches have been extensively investigated to obtain specific and sensitive methods to detect pathogens in food, but they are often not easy to perform and require trained personnel. This work aims to propose a textile organic electrochemical transistor-based (OECT) biosensor to detect L. monocytogenes in food samples. The analyses were performed with culture-based methods, Listeria Precis™ method, PCR, and our textile OECT biosensor which used poly(3,4-ethylenedioxythiophene) (PEDOT):polystyrene sulfonate (PSS) (PEDOT:PSS) for doping the organic channel. Atomic force microscopy (AFM) was used to obtain topographic maps of the gold gate. The electrochemical activity on gate electrodes was measured and related to the concentration of DNA extracted from samples and hybridized to the specific capture probe immobilized onto the gold surface of the gate. This assay reached a limit of detection of 1.05 ng/μL, corresponding to 0.56 pM of L. monocytogenes ATCC 7644, and allowed the specific and rapid detection of L. monocytogenes in the analyzed samples. Keypoints: • Textile organic electrochemical transistors functionalized with a specific DNA probe • AFM topographic and surface potential maps of a functionalized gold gate surface • Comparison between the Listeria monocytogenes Precis™ method and an OECT biosenso

Arabidopsis defense against the pathogenic fungus drechslera gigantea is dependent on the integrity of the unfolded protein response

Drechslera gigantea Heald &amp; Wolf is a worldwide-spread necrotrophic fungus closely related to the Bipolaris genus, well-known because many member species provoke severe diseases in cereal crops and studied because they produce sesterpenoid phytoxins named ophiobolins which possess interesting biological properties. The unfolded protein response (UPR) is a conserved mechanism protecting eukaryotic cells from the accumulation of unfolded/misfolded proteins in the endoplasmic reticulum (ER). In plants, consolidated evidence supports the role of UPR in the tolerance to abiotic stress, whereas much less information is available concerning the induction of ER stress by pathogen infection and consequent UPR elicitation as part of the defense response. In this study, the infection process of D. gigantea in Arabidopsis thaliana wild type and UPR-defective bzip28 bzip60 double mutant plants was comparatively investigated, with the aim to address the role of UPR in the expression of resistance to the fungal pathogen. The results of confocal microscopy, as well as of qRT-PCR transcript level analysis of UPR genes, proteomics, microRNAs expression profile and HPLC-based hormone analyses demonstrated that ophiobolin produced by the fungus during infection compromised ER integrity and that impairment of the IRE1 /bZIP60 pathway of UPR hampered the full expression of resistance, thereby enhancing plant susceptibility to the pathogen