24 research outputs found
Antifungal activity of epithelial secretions from selected frog species of South Africa
Resistance to antibiotics has been acknowledged as a major global public health problem. The use of antimicrobial peptides to provide alternatives to combat multi-drug antibiotic resistance is beginning to attract increasing attention. The high diversity of amphibian skin peptides renders anurans an important potential source for the discovery of novel pharmacophores. This study aimed to investigate the antifungal activity of skin secretions from selected frogs (Amietia fuscigula, Strongylopus grayi and Xenopus laevis) and one toad (Amietophrynus pantherinus) of the south Western Cape Province of South Africa. Initially, different extraction techniques for the collection of skin secretions were tested and optimized, thereafter the extracts were tested against three fungal species of medical and agricultural importance that is, Candida albicans, Fusarium verticillioides and Aspergillus flavus. Chemical stimulation gave the best yield by mass, and secretions from A. fuscigula showed the best activity with an MIC of 40 ÎŒg / ml against C. albicans and 200 ÎŒg / ml against A. flavus. In general, C. albicans and A. flavus were the most sensitive while F. verticillioides was the most resistant. From this study it appears that bioprospecting of South African frog species has the potential to yield potential therapeutic lead agents.Key words: Antifungal, African anurans, antimicrobial peptides (AMP), Candida albicans, Aspergillus flavus, bioprospecting, minimum inhibitory concentrations (MIC)
Relative severity of fumonisin contamination of cereal crops in West Africa
Published online: 18 Sep 2015Traditional and improved varieties of maize, pearl millet and sorghum were planted by small-scale farmers under the direction of the International Institute for Tropical Agriculture in two Nigerian agro-ecological zones: the Sudan Savanna and the Northern Guinea Savanna. Samples were collected for the determination of Fusarium infection and fumonisin (B1, B2 and B3) contamination. A previous paper reported Aspergillus infection and aflatoxin contamination of these samples. Fusarium infection levels, measured by per cent kernels infected, were modest with mean levels for the above cereals of 16% ± 11% (SD), 12% ± 7% and 13% ± 16%, respectively. However, the Fusarium species recovered from maize were predominantly the fumonisin producers F. verticillioides and F. proliferatum, together making an infection rate of 15% ± 10%, whereas these species were present to a limited extent only in the other two cereals, 1% ± 1% for pearl millet and 2% ± 6% for sorghum. Fumonisin contamination was variable but reflected the diversity of Fusarium producers in these three cereals. Mean levels were 228 ± 579 ”g kgâ1 (range < 5â2860 ”g kgâ1) for maize, 18 ± 7 ”g kgâ1 (range = 6â29 ”g kgâ1) for pearl millet and 131 ± 270 ”g kgâ1 (range < 5â1340 ”g kgâ1) for sorghum. Together with previous results on aflatoxin, this study confirmed the co-occurrence of aflatoxins and fumonisins in maize as well as in the traditional African cereals, millet and sorghum (89% co-occurrence across all three cereals). The low fumonisin levels may be ascribed to the use of good agricultural practices. Of the Fusarium species present, those in maize consisted mainly of fumonisin producers, the opposite of what was observed in pearl millet and sorghum. It is concluded that replacement of maize by pearl millet and sorghum could improve food safety with regards to aflatoxin B and fumonisin B exposure
Invasive fungal infections, other than Candida and Cryptococcus, from 2008 to 2011, at Tygerberg Hospital in the Western Cape, South Africa
Incidence of Fusarium verticillioides and levels of fumonisins in corn from main production areas in Iran
ArticleA total of 52 corn samples collected in 2000 from four main corn production provinces of Iran (Fars, Kermanshah, Khuzestan, and Mazandaran) were analyzed for contamination with Fusarium verticillioides and fumonisins (FB(1), FB(2), FB(3), and 3-epi-FB(3)). The mean incidence of F. verticillioides (percent of kernels infected) for these four areas was 26.7, 21.4, 24.9, and 59.0%, respectively. The incidence in Mazandaran was significantly (p < 0.05) above that of the other areas. All samples from Mazandaran were contaminated with fumonisins with a mean level of total fumonisins of 10674 microg/kg. In contrast, the incidence of fumonisin contamination above 10 microg/kg was 53 (8/15), 42 (5/12), and 57% (8/14) in the samples from Fars, Kermanshah, and Khuzestan, respectively, and the corresponding mean total fumonisin levels were 215, 71, and 174 microg/kg, respectively. No statistical differences (p > 0.05) were observed in the fumonisin levels of the corn samples from these three provinces, which were significantly (p < 0.05) lower than the fumonisin contamination in samples from Mazandaran
Cryptococcus neoformans and Cryptococcus gattii isolated from woody debris samples collected from trees within a public garden in Cape Town, South Africa
Molecular characterization of Fusarium globosum strains from South African maize and Japanese wheat
The fungus Fusarium globosum was first isolated from maize in South Africa and subsequently from wheat in Japan. Here, multiple analyses revealed that, despite morphological similarities, South African maize and Japanese wheat isolates of the fungus exhibit multiple differences. An amplified fragment length polymorphism-based similarity index for the two groups of isolates was only 45%. Most maize isolates produced relatively high levels of fumonisins, whereas wheat isolates produced little or no fumonisins. The fumonisin biosynthetic gene FUM1 was detected in maize isolates by Southern blot analysis but not in the wheat isolates. In addition, most of the maize isolates produced sclerotia, and all of them produced large orange to dark purple sporodochia in carrot agar culture, whereas wheat isolates did not produce either structure. In contrast, individual isolates from both maize and wheat carried markers for both mating type idiomorphs, which indicates that the fungus may be homothallic. However, a sexual stage of F. globosum was not formed under standard self-fertilization conditions developed for other homothallic species of Fusarium. The inability to produce the sexual stage is consistent with the high similarity of 87â100% and GST index of 1.72 for the maize isolates, which suggests that these isolates are undergoing asexual but not sexual reproduction. Together, the results suggest that the South African maize and Japanese wheat isolates of F. globosum are distinct populations and could be different species
Fusarium inhibition by wild populations of the medicinal plant Salvia africana-lutea L. linked to metabolomic profiling
Publication of this article was funded by the Stellenbosch University Open Access Fund.The original publication is available at http://www.biomedcentral.com/1472-6882/14/99Abstract
Background: Salvia africana-lutea L., an important medicinal sage used in the Western Cape (South Africa), can be
termed a âbroad-spectrum remedyâ suggesting the presence of a multiplicity of bioactive metabolites. This study
aimed at assessing wild S. africana-lutea populations for chemotypic variation and anti-Fusarium properties.
Methods: Samples were collected from four wild growing population sites (Yzerfontein, Silwerstroomstrand,
Koeberg and Brackenfell) and one garden growing location in Stellenbosch. Their antifungal activities against
Fusarium verticillioides (strains: MRC 826 and MRC 8267) and F. proliferatum (strains: MRC 6908 and MRC 7140)
that are aggressive mycotoxigenic phytopathogens were compared using an in vitro microdilution assay. To
correlate antifungal activity to chemical profiles, three techniques viz. Gas chromatography-mass spectrometry
(GC-MS); Liquid chromatography-mass spectrometry (LC-MS) and 1H Nuclear Magnetic Resonance (NMR) were
employed. Principal Component Analysis (PCA) was applied to the NMR data. The partial least squares-discriminant
analysis (PLS-DA) was used to integrate LC-MS and NMR data sets. All statistics were performed with the SIMCA-P + 12.0
software.
Results: The dichloromethane:methanol (1:1; v/v) extracts of the plant species collected from Stellenbosch
demonstrated the strongest inhibition of F. verticillioides and F. proliferatum with minimum inhibitory concentration
(MIC) values of 0.031 mg ml-1 and 0.063 mg ml-1 respectively. GC-MS showed four compounds which were unique to
the Stellenbosch extracts. By integrating LC-MS and 1H NMR analyses, large chemotype differences leading to samples
grouping by site when a multivariate analysis was performed, suggested strong plant-environment interactions as
factors influencing metabolite composition. Signals distinguishing the Stellenbosch profile were in the aromatic
part of the 1H NMR spectra.
Conclusions: This study shows the potential of chemotypes of Salvia africana-lutea in controlling fungal growth
and consequently mycotoxin production. Products for use in the agricultural sector may be developed from such
chemotypes.Stellenbosch UniversityPublishers' Versio