An approach to high speed ship ride quality simulation

The high speeds attained by certain advanced surface ships result in a spectrum of motion which is higher in frequency than that of conventional ships. This fact along with the inclusion of advanced ride control features in the design of these ships resulted in an increased awareness of the need for ride criteria. Such criteria can be developed using data from actual ship operations in varied sea states or from clinical laboratory experiments. A third approach is to simulate ship conditions using measured or calculated ship motion data. Recent simulations have used data derived from a math model of Surface Effect Ship (SES) motion. The model in turn is based on equations of motion which have been refined with data from scale models and SES of up to 101 600-kg (100-ton) displacement. Employment of broad band motion emphasizes the use of the simulators as a design tool to evaluate a given ship configuration in several operational situations and also serves to provide data as to the overall effect of a given motion on crew performance and physiological status

Melt droplet formation in energetic impacts

Impacts between rocky bodies at velocities exceeding about 15 km/sec are capable of melting or vaporizing both the impacting object and a portion of the target. Geological materials initially shocked to high pressure approach the liquid-vapor phase boundary from the liquid side as they decompress, breaking up into an expanding spray of liquid droplets. A simple theory is presented for estimating the sizes of these droplets as a function of impactor size and velocity. It is shown that these sizes are consistent with observations of microtektites and spherules found in the Cretaceous-Tertiary boundary layer, the Acraman impact structure, Archean beds in South Africa and lunar regolith. The model may also apply to the formation of chondrules

Production of impact melt in craters on Venus, Earth, and the moon

Impact craters imaged by Magellan clearly show large amounts of flow-like ejecta whose morphology suggests that the flows comprise low-viscosity material. It was suggested that this material may be either turbidity flows or very fine-grained ejecta, flows of ejecta plus magma, or impact melts. The last of these hypotheses is considered. If these flows are composed of impact melts, there is much more melt relative to the crater volume than is observed on the moon. The ANEOS equation of state program was used for dunite to estimate the shock pressures required for melting, with initial conditions appropriate for Venus, Earth, and the moon. A simple model was then developed, based on the Z-model for excavation flow and on crater scaling relations that allow to estimate the ratio of melt ejecta to total ejecta as a function of crater size on the three bodies

New Visualization Techniques for Multi-Dimensional Variables in Complex Physical Domains

This work presents the new Synthesized Cell Texture (SCT) algorithm for visualizing related multiple scalar value fields within the same 3D space. The SCT method is particularly well suited to scalar quantities that could be represented in the physical domain as size fractionated particles, such as in the study of sedimentation, atmospheric aerosols, or precipitation. There are two components to this contribution. First a Scaling and Distribution (SAD) algorithm provides a means of specifying a multi-scalar field in terms of a maximum cell resolution (or density of represented values). This information is used to scale the multi-scalar field values for each 3D cell to the maximum values found throughout the data set, and then randomly distributes those values as particles varying in number, size, color, and opacity within a 2D cell slice. This approach facilitates viewing of closely spaced layers commonly found in sigma-coordinate grids. The SAD algorithm can be applied regardless of how the particles are rendered. The second contribution provides the Synthesized Cell Texture (SCT) algorithm to render the multi-scalar values. In this approach, a texture is synthesized from the location information computed by the SAD algorithm, which is then applied to each cell as a 2D slice within the volume. The SCT method trades off computation time (to synthesize the texture) and texture memory against the number of geometric primitives that must be sent through the graphics pipeline of the host system. Analysis results from a user study prove the effectiveness of the algorithm as a browsing method for multiple related scalar fields. The interactive rendering performance of the SCT method is compared with two common basic particle representations: flat-shaded color-mapped OpenGL points and quadrilaterals. Frame rate statistics show the SCT method to be up to 44 times faster, depending on the volume to be displayed and the host system. The SCT method has been successfully applied to oceanographic sedimentation data, and can be applied to other problem domains as well. Future enhancements include the extension to time-varying data and parallelization of the texture synthesis component to reduce startup time

Transfer of Sulfur from IscS to IscU during Fe/S Cluster Assembly

The cysteine desulfurase enzymes NifS and IscS provide sulfur for the biosynthesis of Fe/S proteins. NifU and IscU have been proposed to serve as template or scaffold proteins in the initial Fe/S cluster assembly events, but the mechanism of sulfur transfer from NifS or IscS to NifU or IscU has not been elucidated. We have employed [35S]cysteine radiotracer studies to monitor sulfur transfer between IscS and IscU from Escherichia coli and have used direct binding measurements to investigate interactions between the proteins. IscS catalyzed transfer of 35S from [35S]cysteine to IscU in the absence of additional thiol reagents, suggesting that transfer can occur directly and without involvement of an intermediate carrier. Surface plasmon resonance studies and isothermal titration calorimetry measurements further revealed that IscU binds to IscS with high affinity (Kd ~2 µM) in support of a direct transfer mechanism. Transfer was inhibited by treatment of IscU with iodoacetamide, and 35S was released by reducing reagents, suggesting that transfer of persulfide sulfur occurs to cysteinyl groups of IscU. A deletion mutant of IscS lacking C-terminal residues 376-413 (IscSDelta 376-413) displayed cysteine desulfurase activity similar to the full-length protein but exhibited lower binding affinity for IscU, decreased ability to transfer 35S to IscU, and reduced activity in assays of Fe/S cluster assembly on IscU. The findings with IscSDelta 376-413 provide additional support for a mechanism of sulfur transfer involving a direct interaction between IscS and IscU and suggest that the C-terminal region of IscS may be important for binding IscU

Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target

Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health. NRPSs are found in both bacteria and fungi but most of the research to date has focused on the bacterial enzymes. This is largely due to the technical challenges in producing active fungal NRPSs, which stem from their large size and multidomain nature. In order to target fungal NRPS domains for biochemical and structural characterisation, we tackled this challenge by using the cloning and expression tools of structural genomics to screen the many variables that can influence the expression and purification of proteins. Using these tools we have screened 32 constructs containing 16 different fungal NRPS domains or domain combinations for expression and solubility. Two of these yielded soluble protein with one, the third adenylation domain of the SidN NRPS (SidNA3) from the grass endophyte Neotyphodium lolii, being tractable for purification using Ni-affinity resin. The initial purified protein exhibited poor solution behaviour but optimisation of the expression construct and the buffer conditions used for purification, resulted in stable recombinant protein suitable for biochemical characterisation, crystallisation and structure determination

Systematic differences between Cochrane and non-Cochrane meta-analyses on the same topic: a matched pair analysis

BACKGROUND: Meta-analyses conducted via the Cochrane Collaboration adhere to strict methodological and reporting standards aiming to minimize bias, maximize transparency/reproducibility, and improve the accuracy of summarized data. Whether this results in differences in the results reported by meta-analyses on the same topic conducted outside the Cochrane Collaboration is an open question. METHODS: We conducted a matched-pair analysis with individual meta-analyses as the unit of analysis, comparing Cochrane and non-Cochrane reviews. Using meta-analyses from the cardiovascular literature, we identified pairs that matched on intervention and outcome. The pairs were contrasted in terms of how frequently results disagreed between the Cochrane and non-Cochrane reviews, whether effect sizes and statistical precision differed systematically, and how these differences related to the frequency of secondary citations of those reviews. RESULTS: Our search yielded 40 matched pairs of reviews. The two sets were similar in terms of which was first to publication, how many studies were included, and average sample sizes. The paired reviews included a total of 344 individual clinical trials: 111 (32.3%) studies were included only in a Cochrane review, 104 (30.2%) only in a non-Cochrane review, and 129 (37.5%) in both. Stated another way, 62.5% of studies were only included in one or the other meta-analytic literature. Overall, 37.5% of pairs had discrepant results. The most common involved shifts in the width of 95% confidence intervals that would yield a different statistical interpretation of the significance of results (7 pairs). Additionally, 20% differed in the direction of the summary effect size (5 pairs) or reported greater than a 2-fold difference in its magnitude (3 pairs). Non-Cochrane reviews reported significantly higher effect sizes (P < 0.001) and lower precision (P < 0.001) than matched Cochrane reviews. Reviews reporting an effect size at least 2-fold greater than their matched pair were cited more frequently. CONCLUSIONS: Though results between topic-matched Cochrane and non-Cochrane reviews were quite similar, discrepant results were frequent, and the overlap of included studies was surprisingly low. Non-Cochrane reviews report larger effect sizes with lower precision than Cochrane reviews, indicating systematic differences, likely reflective of methodology, between the two types of reviews that could generate different interpretations of the interventions under question

Teaching Information Literacy Using the Short Story

Stories are powerful teaching tools because of their potential to stimulate the imagination of students and engage them with the material. The short story gives meaning to abstract concepts, aids memory, makes learning fun, and is time efficient. This article explains the approach to teaching information literacy through the use of short stories, including how to create vivid connections to the Association of College and Research Libraries’ Information Literacy Competency Standards for Higher Education. Provides course instructors with examples of how the short story can be used as a platform to discuss information literacy standards

Hsc66 substrate specificity is directed toward a discrete region of the iron-sulfur cluster template protein IscU

Hsc66 and Hsc20 comprise a specialized chaperone system important for the assembly of iron-sulfur clusters in Escherchia coli. Only a single substrate, the Fe/S template protein IscU, has been identified for the Hsc66/Hsc20 system, but the mechanism by which Hsc66 selectively binds IscU is unknown. We have investigated Hsc66 substrate specificity using phage display and a peptide array of IscU. Screening of a heptameric peptide phage display library revealed that Hsc66 prefers peptides with a centrally located Pro-Pro motif. Using a cellulose-bound peptide array of IscU we determined that Hsc66 interacts specifically with a region (residues 99-103, LPPVK) that is invariant among all IscU family members. A synthetic peptide (ELPPVKIHC) corresponding to IscU residues 98-106 behaves in a similar manner to native IscU, stimulating the ATPase activity of Hsc66 with similar affinity as IscU, preventing Hsc66 suppression of bovine rhodanese aggregation, and interacting with the peptide-binding domain of Hsc66. Unlike native IscU, however, the synthetic peptide is not bound by Hsc20 and does not synergistically stimulate Hsc66 ATPase activity with Hsc20. Our results indicate that Hsc66 and Hsc20 recognize distinct regions of IscU and further suggest that Hsc66 will not bind LPPVK motifs with high affinity in vivo unless they are in the context of native IscU and can be directed to Hsc66 by Hsc20