Uticaj niskofrekventnog magnetnog polja (10-50 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae

The analysis of the electric, magnetic and electromagnetic fields influence on microorganisms is a very popular research topic, since these fields could potentially act as stressors and affect the microbial metabolism and survival. The aim of this work was to investigate the influence of the low frequency magnetic field (MF) with scan regime from 10 Hz to 50 Hz on S. cerevisiae respiration. The experiment was performed in five replicates and monitored using the Micro-Oxymax® respirometer. All five experiments showed lower cumulative O2 consumption in MF exposed samples, compared to the control sample and inconsistent cumulative CO2 production. However, these differences in O2 consumption and CO2 production were statistically significant. Even though additional experiments are necessary, these results strongly suggest that this is a good basis for further investigation in this field.Ispitivanje uticaja električnog, magnetnog i elektromagnetnog polja na mikroorganizme je veoma aktuelni predmet istrazivanja, jer ova fizička polja potencijalno deluju kao faktori stresa i tako utiču na mikrobni metabolizam, ponašanje i preživljavanje. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja (MP) sa konstantnim intervalom skeniranja od 10 do 50 Hz na respiraciju ćelija kvasca, S. cerevisiae. Eksperiment je rađen u pet ponavljanja i praćen Micro-Oxymax® respirometrom. Kumulativna potrošnja kiseonika je bila manja kod ćelija izloženih MP u svih pet ponavljanja, dok je produkcija CO2 bila nekonzistentna. Međutim, ove razlike u potrošnji O2 i produkciji CO2 su statistički značajne. Iako su dodatna ispitivanja neophodna, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.Related to: [http://cer.ihtm.bg.ac.rs/handle/123456789/3288

The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth

The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower cumulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3321

Uticaj niskofrekventnog magnetnog polja (10-50 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae

The analysis of the electric, magnetic and electromagnetic fields influence on microorganisms is a very popular research topic, since these fields could potentially act as stressors and affect the microbial metabolism and survival. The aim of this work was to investigate the influence of the low frequency magnetic field (MF) with scan regime from 10 Hz to 50 Hz on S. cerevisiae respiration. The experiment was performed in five replicates and monitored using the Micro-Oxymax® respirometer. All five experiments showed lower cumulative O2 consumption in MF exposed samples, compared to the control sample and inconsistent cumulative CO2 production. However, these differences in O2 consumption and CO2 production were statistically significant. Even though additional experiments are necessary, these results strongly suggest that this is a good basis for further investigation in this field.Ispitivanje uticaja električnog, magnetnog i elektromagnetnog polja na mikroorganizme je veoma aktuelni predmet istrazivanja, jer ova fizička polja potencijalno deluju kao faktori stresa i tako utiču na mikrobni metabolizam, ponašanje i preživljavanje. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja (MP) sa konstantnim intervalom skeniranja od 10 do 50 Hz na respiraciju ćelija kvasca, S. cerevisiae. Eksperiment je rađen u pet ponavljanja i praćen Micro-Oxymax® respirometrom. Kumulativna potrošnja kiseonika je bila manja kod ćelija izloženih MP u svih pet ponavljanja, dok je produkcija CO2 bila nekonzistentna. Međutim, ove razlike u potrošnji O2 i produkciji CO2 su statistički značajne. Iako su dodatna ispitivanja neophodna, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.Related to: [http://cer.ihtm.bg.ac.rs/handle/123456789/3288

Uticaj niskofrekventnog magnetnog polja (10-50 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae

The analysis of the electric, magnetic and electromagnetic fields influence on microorganisms is a very popular research topic, since these fields could potentially act as stressors and affect the microbial metabolism and survival. The aim of this work was to investigate the influence of the low frequency magnetic field (MF) with scan regime from 10 Hz to 50 Hz on S. cerevisiae respiration. The experiment was performed in five replicates and monitored using the Micro-Oxymax® respirometer. All five experiments showed lower cumulative O2 consumption in MF exposed samples, compared to the control sample and inconsistent cumulative CO2 production. However, these differences in O2 consumption and CO2 production were statistically significant. Even though additional experiments are necessary, these results strongly suggest that this is a good basis for further investigation in this field.Ispitivanje uticaja električnog, magnetnog i elektromagnetnog polja na mikroorganizme je veoma aktuelni predmet istrazivanja, jer ova fizička polja potencijalno deluju kao faktori stresa i tako utiču na mikrobni metabolizam i preživljavanje. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja (MP) sa konstantnim intervalom skeniranja od 10 do 50 Hz na respiraciju ćelija kvasca, S. cerevisiae. Eksperiment je rađen u pet ponavljanja i praćen Micro-Oxymax® respirometrom. Kumulativna potrošnja kiseonika je bila manja kod ćelija izloženih MP u svih pet ponavljanja, dok je produkcija CO2 bila nekonzistentna. Međutim, ove razlike u potrošnji O2 i produkciji CO2 su statistički značajne. Iako su dodatna ispitivanja neophodna, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.Related to: [http://cer.ihtm.bg.ac.rs/handle/123456789/3289

Uticaj niskofrekventnog magnetnog polja (10-50 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae

The analysis of the electric, magnetic and electromagnetic fields influence on microorganisms is a very popular research topic, since these fields could potentially act as stressors and affect the microbial metabolism and survival. The aim of this work was to investigate the influence of the low frequency magnetic field (MF) with scan regime from 10 Hz to 50 Hz on S. cerevisiae respiration. The experiment was performed in five replicates and monitored using the Micro-Oxymax® respirometer. All five experiments showed lower cumulative O2 consumption in MF exposed samples, compared to the control sample and inconsistent cumulative CO2 production. However, these differences in O2 consumption and CO2 production were statistically significant. Even though additional experiments are necessary, these results strongly suggest that this is a good basis for further investigation in this field.Ispitivanje uticaja električnog, magnetnog i elektromagnetnog polja na mikroorganizme je veoma aktuelni predmet istrazivanja, jer ova fizička polja potencijalno deluju kao faktori stresa i tako utiču na mikrobni metabolizam i preživljavanje. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja (MP) sa konstantnim intervalom skeniranja od 10 do 50 Hz na respiraciju ćelija kvasca, S. cerevisiae. Eksperiment je rađen u pet ponavljanja i praćen Micro-Oxymax® respirometrom. Kumulativna potrošnja kiseonika je bila manja kod ćelija izloženih MP u svih pet ponavljanja, dok je produkcija CO2 bila nekonzistentna. Međutim, ove razlike u potrošnji O2 i produkciji CO2 su statistički značajne. Iako su dodatna ispitivanja neophodna, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.Related to: [http://cer.ihtm.bg.ac.rs/handle/123456789/3289

Uticaj niskofrekventnog magnetnog polja (10-1000 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae

The effects of electric, magnetic or electromagnetic fields on different microbes have become a very popular topic nowadays, mostly because the fields could potentially affect the survival of the microbial cells as well as their behavior and metabolism. In this paper the influence of the magnetic field with constant low frequency scan regime from 10 to 1000 Hz on yeast cells S. cerevisiae respiration activity was examined using Micro-Oxymax® respirometer. Experiments were performed in five replicates. All five experiments showed lower cumulative O2 consumption in magnetic field exposed samples, compared to the control sample and inconsistent cumulative CO2 production. The paired two sample onetail T-test showed statistically significant differences between control and magnetic field sample for cumulative O2 consumption, but not for the CO2 production. Even though additional experiments are neccesary to clarify the differencies in CO2 production, these preliminary findings strongly suggest that obtained results represent a good basis for further investigations in this field.Veoma popularna tema današnjice je ispitivanje električnog, magnetnog i elektromagnetnog polja na različite mikroorganizme, jer pomenuta fizička polja potencijalno deluju kao faktori stresa i tako utiču na njihovo preživljavanje, ponašanje i metabolizam. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja sa konstantnim intervalom skeniranja od 10 do 1000 Hz na respiraciju ćelija kvasca, Saccharomyces cerevisiae. Eksperimenti su rađeni u pet ponavljanja i kumulativna potrošnja O2 i produkcija CO2 praćena je pomoću Micro-Oxymax® respirometra. U svih pet ponavljanja, ćelije koje su bile izložene magnetnom polju pokazale su manju kumulativnu potrošnju kiseonika u poređenju sa uzorcima van magnetnog polja i nekonzistentnu produkciju CO2. Rezultati su obrađeni uporednim jednosmernim T-testom, koji je pokazao da postoje statistički značajne razlike u kumulativnoj potrošnji O2 između kontrolnih ćelija i onih izloženih magnetnom polju, što nije slučaj sa kumulativnom produkcijom CO2. Iako su dodatna ispitivanja neophodna da se objasni nekonzistentnost produkcije CO2, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti

Influence of low frequency ranges of magnetic field on Saccharomyces cerevisiae respiration

In this paper influence of the low frequency magnetic field on Saccharomyces cerevisiae respiration was examined. Influence of four low frequency magnetic field ranges was examined: 10-200 Hz, 200-300 Hz, 300-650 Hz and 650-1000 Hz. All analyzed frequency ranges gave the same influence on the yeast cells respiration

The pH measurement with glass electrode in an electromagnetic field

Measurements of pH values of buffer solutions (pH 4.0, 7.0 and 10.0) and distilled water have been performed with a glass electrode in electromagnetic field at the frequency interval of 10 MHz to 200 MHz and the output power of dispersed and reflected electromagnetic radiation of 0.01 W to 3 W. In all the cases, there occurred a reduction of pH values, i.e. a "recorded pH value" was obtained. The reduction appears within the applied frequency interval reaching extreme values at specific frequencies. The reduction of the pH values increases with the radiation power and depend of the solution buffer capacity. The effect of electromagnetic field on pH value change is exerted dominantly through the influence on glass electrodes

ASH DUST CONCENTRATION IN THE VICINITY OF THE ASH DISPOSAL SITE DEPENDING ON THE SIZE OF THE POND

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Influence of low frequency ranges of magnetic field on Saccharomyces cerevisiae respiration

In this paper influence of the low frequency magnetic field on Saccharomyces cerevisiae respiration was examined. Influence of four low frequency magnetic field ranges was examined: 10-200 Hz, 200-300 Hz, 300-650 Hz and 650-1000 Hz. All analyzed frequency ranges gave the same influence on the yeast cells respiration