Boolean versus continuous dynamics on simple two-gene modules

We investigate the dynamical behavior of simple modules composed of two genes with two or three regulating connections. Continuous dynamics for mRNA and protein concentrations is compared to a Boolean model for gene activity. Using a generalized method, we study within a single framework different continuous models and different types of regulatory functions, and establish conditions under which the system can display stable oscillations. These conditions concern the time scales, the degree of cooperativity of the regulating interactions, and the signs of the interactions. Not all models that show oscillations under Boolean dynamics can have oscillations under continuous dynamics, and vice versa.Comment: 8 pages, 10 figure

Sol–gel synthesis and thermal behavior of bioactive ferrous citrate–silica hybrid materials

Imbalance of the iron level in the body causes several diseases. In particular, the low level of iron, during pregnancy, is responsible for the iron deficiency anemia, and even of neurodegenerative diseases. Although the treatment of iron deficiency anemia with oral iron supplements has been known, this problem still afflicts many people. The aim of this work was the development of a system able to release ferrous ions in a controlled manner. Controlled drug release for medical applications, indeed, appears to be a very interesting alternative to a systemic therapy because it is assurance of treatment continuity and drug stability and optimizes drug absorption. For this purpose, ferrous citrate (Fe(II)C) was synthesized by a redox reaction between iron powder and citric acid. Fourier transform infrared spectroscopy (FTIR), 1,10-phenanthroline and sodium thiocyanate colorimetric assays confirmed that only Fe(II)C was obtained by redox reaction. Afterward, obtained Fe(II)C was embedded within a SiO2 matrix in different mass percentage, by means of a sol–gel route. FTIR spectroscopy and simultaneous thermogravimetry/first-order derivative of thermogravimetry were used to confirm the Fe(II)C presence in the silica matrix and to investigate the thermal behavior of the sol–gel materials, respectively. The bioactivity test carried out by soaking the synthesized drug delivery systems in a simulated body fluid showed that the biological properties of the silica matrix are not modified by the presence of Fe(II)C

A Markov Chain Monte Carlo approach to the study of massive black hole binary systems with LISA

The Laser Interferometer Space Antenna (LISA) will produce a data stream containing a vast number of overlapping sources: from strong signals generated by the coalescence of massive black hole binary systems to much weaker radiation form sub-stellar mass compact binaries and extreme-mass ratio inspirals. It has been argued that the observation of weak signals could be hampered by the presence of loud ones and that they first need to be removed to allow such observations. Here we consider a different approach in which sources are studied simultaneously within the framework of Bayesian inference. We investigate the simplified case in which the LISA data stream contains radiation from a massive black hole binary system superimposed over a (weaker) quasi-monochromatic waveform generated by a white dwarf binary. We derive the posterior probability density function of the model parameters using an automatic Reversible Jump Markov Chain Monte Carlo algorithm (RJMCMC). We show that the information about the sources and noise are retrieved at the expected level of accuracy without the need of removing the stronger signal. Our analysis suggests that this approach is worth pursuing further and should be considered for the actual analysis of the LISA data.Comment: submitted to cqg as GWDAW-10 conference proceedings, 10 pages, 4 figures, some changes to plots and numerical detail

Data Processing for LISA's Laser Interferometer Tracking System (LITS)

The purpose of this paper is twofold. First, we will present recent results on the data processing for LISA, including algorithms for elimination of clock jitter noise and discussion of the generation of the data averages that will eventually need to be telemetered to the ground. Second, we will argue, based partly on these results, that a laser interferometer tracking system (LITS) that employs independent lasers in each spacecraft is preferable for reasons of simplicity to that in which the lasers in two of the spacecraft are locked to the incoming beam from the third.Comment: 5 pages, Proceedings of the Third LISA Symposium (Golm, Germany, 2000

Capillary Electrophoresis: A Method for Evolution and Analysis of Molecular Recognition Elements

Molecular Recognition Elements (MREs) are compounds capable of binding a variety of inorganic and biological molecules. These MREs have a variety of applications including incorporation into biosensors, drug discovery, diagnostic testing, and therapeutics. MREs are evolved via a generalized process known as the Systematic Evolution of Ligands by Exponential Enrichment, or SELEX. Traditional SELEX techniques have limited the variety MREs which can be derived for a given target, and can take several days to complete. Capillary Electrophoresis has emerged as an alternative method for evolution of MREs. It allows for evolution of MREs with increased affinity for a given target in significantly fewer rounds of selection. In addition, a range of capillary electrophoresis binding assays have been developed which can very accurately determine dissociation constant values for MRE/target complexes. Capillary electrophoresis has greatly influenced both evolution and characterization of MREs over the past decade. It will likely continue to facilitate the development of MREs for applications which were previously inconceivable using traditional SELEX techniques

LISA Response Function and Parameter Estimation

We investigate the response function of LISA and consider the adequacy of its commonly used approximation in the high-frequency range of the observational band. We concentrate on monochromatic binary systems, such as white dwarf binaries. We find that above a few mHz the approxmation starts becoming increasingly inaccurate. The transfer function introduces additional amplitude and phase modulations in the measured signal that influence parameter estmation and, if not properly accounted for, lead to losses of signal-to-noise ratio.Comment: 4 pages, 2 figures, amaldi 5 conference proceeding

Media use during adolescence: the recommendations of the Italian Pediatric Society.

BACKGROUND: The use of media device, such as smartphone and tablet, is currently increasing, especially among the youngest. Adolescents spend more and more time with their smartphones consulting social media, mainly Facebook, Instagram and Twitter because. Adolescents often feel the necessity to use a media device as a means to construct a social identity and express themselves. For some children, smartphone ownership starts even sooner as young as 7 yrs, according to internet safety experts. MATERIAL AND METHODS: We analyzed the evidence on media use and its consequences in adolescence. RESULTS: In literature, smartphones and tablets use may negatively influences the psychophysical development of the adolescent, such as learning, sleep and sigh. Moreover, obesity, distraction, addiction, cyberbullism and Hikikomori phenomena are described in adolescents who use media device too frequently. The Italian Pediatric Society provide action-oriented recommendations for families and clinicians to avoid negative outcomes. CONCLUSIONS: Both parents and clinicians should be aware of the widespread phenomenon of media device use among adolescents and try to avoid psychophysical consequences on the youngest

ESA, iron therapy and new drugs: Are there new perspectives in the treatment of anaemia?

Anemia is a well-known consequence of chronic kidney disease (CKD); it is mainly due to a relative insufficiency of erythropoietin synthesis by the failing kidneys. Over the years, the combination of erythropoiesis stimulating agents (ESA) and iron has become the standard of care of anemia. All ESAs effectively increase hemoglobin (Hb) levels in a substantial percentage of patients. However, in the last decade, their use has been surrounded by safety issues in increased cardiovascular risk, especially when used at high doses in inflamed and hyporesponsive patients. This has led to the definition of a more cautious Hb target. Iron deficiency is very frequent in CKD patients, with a higher frequency in non-dialysis patients. Traditionally, iron supplementation is mostly used as supportive therapy for anemia control. However, the concept is growing that intravenous iron therapy per se could be beneficial in the presence of heart failure. A new class of drugs, prolyl hydroxylase domain (PHD) inhibitors (PHD inhibitors) is becoming available for the treatment of anemia in CKD patients. Theoretically, these agents have a number of advantages, the main ones being that of stimulating the synthesis of endogenous erythropoietin and increasing iron avail-ability. The impact of their future use in clinical practice is still to be defined. Another possible strategy could be targeting serum hepcidin and its related pathways. This possibility is fascinating from the scientific point of view, but at present its development phase is still far from clinical application

The Effect of the LISA Response Function on Observations of Monochromatic Sources

The Laser Interferometer Space Antenna (LISA) is expected to provide the largest observational sample of binary systems of faint sub-solar mass compact objects, in particular white-dwarfs, whose radiation is monochromatic over most of the LISA observational window. Current astrophysical estimates suggest that the instrument will be able to resolve about 10000 such systems, with a large fraction of them at frequencies above 3 mHz, where the wavelength of gravitational waves becomes comparable to or shorter than the LISA arm-length. This affects the structure of the so-called LISA transfer function which cannot be treated as constant in this frequency range: it introduces characteristic phase and amplitude modulations that depend on the source location in the sky and the emission frequency. Here we investigate the effect of the LISA transfer function on detection and parameter estimation for monochromatic sources. For signal detection we show that filters constructed by approximating the transfer function as a constant (long wavelength approximation) introduce a negligible loss of signal-to-noise ratio -- the fitting factor always exceeds 0.97 -- for f below 10mHz, therefore in a frequency range where one would actually expect the approximation to fail. For parameter estimation, we conclude that in the range 3mHz to 30mHz the errors associated with parameter measurements differ from about 5% up to a factor of 10 (depending on the actual source parameters and emission frequency) with respect to those computed using the long wavelength approximation.Comment: replacement version with typos correcte