Osteopontin characterizes bile duct-associated macrophages and correlates with liver fibrosis severity in primary sclerosing cholangitis

Background and Aims: Primary sclerosing cholangitis (PSC) is an immune-mediated cholestatic liver disease for which pharmacological treatment options are currently unavailable. PSC is strongly associated with colitis and a disruption of the gut-liver axis, and macrophages are involved in the pathogenesis of PSC. However, how gut-liver interactions and specific macrophage populations contribute to PSC is incompletely understood. Approach and Results: We investigated the impact of cholestasis and colitis on the hepatic and colonic microenvironment, and performed an in-depth characterization of hepatic macrophage dynamics and function in models of concomitant cholangitis and colitis. Cholestasis-induced fibrosis was characterized by depletion of resident KCs, and enrichment of monocytes and monocyte-derived macrophages (MoMFs) in the liver. These MoMFs highly express triggering-receptor-expressed-on-myeloid-cells-2 (Trem2) and osteopontin (Spp1), markers assigned to hepatic bile duct-associated macrophages, and were enriched around the portal triad, which was confirmed in human PSC. Colitis induced monocyte/macrophage infiltration in the gut and liver, and enhanced cholestasis-induced MoMF-Trem2 and Spp1 upregulation, yet did not exacerbate liver fibrosis. Bone marrow chimeras showed that knockout of Spp1 in infiltrated MoMFs exacerbates inflammation in vivo and in vitro, while monoclonal antibody–mediated neutralization of SPP1 conferred protection in experimental PSC. In human PSC patients, serum osteopontin levels are elevated compared to control, and significantly increased in advanced stage PSC and might serve as a prognostic biomarker for liver transplant-free survival. Conclusions: Our data shed light on gut-liver axis perturbations and macrophage dynamics and function in PSC and highlight SPP1/OPN as a prognostic marker and future therapeutic target in PSC.publishedVersio

Verwerk insecten als halffabricaat

status: publishe

Tyndallisation as a treatment to reduce the bacterial endospore load in mealworms (Tenebrio molitor & Alphitobius diaperinus)

status: publishe

Effect of MAP, ethanol and the combination of MAP and ethanol on the shelf life of sliced bread

status: publishe

Industrial microwave drying of yellow mealworm larvae (Tenebrio molitor): impact on moisture content, water activity and microbial load

status: publishe

Microbiological assessment of blanching and refrigerated storage for fresh edible mealworm larvae (Tenebrio molitor)

status: publishe

Potential of Fermentation and Vacuum Packaging Followed by Chilling to Preserve Black Soldier Fly Larvae (Hermetia illucens)

Black soldier fly larvae (Hermetia illucens) are currently reared at an industrial scale, mainly as a feed ingredient. The logistic chain not only involves the production of larvae, but also stabilisation, storage, and transport. The aim of this work was to study fermentation and vacuum packaging of larvae as potential preservation technologies. For fermentation, blanched larvae were pulverised into a paste, and a starter culture, NaCl, and glucose were added. The mixture was fermented for 7 days at 35 °C and then stored for 14 days at 4 °C and pH and microbial counts were monitored. Vacuum packaging was applied to living, blanched and frozen larvae. After packaging, they were stored for 6–10 days at several temperatures and gas composition, survival (living larvae) and microbial counts (killed larvae) were recorded. Fermentation allows storage of pulverised larvae, but points to consider are a rapid pH reduction and the presence of bacterial endospores. Vacuum packaging did not bring added value over cooling alone. This was the case for all types of larvae investigated. Vacuum packaging is not considered as a valuable preservation technology to pursue for storage and transport of black soldier fly larvae

Identification of bacterial endospores and targeted detection of foodborne viruses in industrially reared insects for food

With edible insects being increasingly produced, food safety authorities have called for the determination of microbiological challenges posed to human health. Here, we find that the bacterial endospore fraction in industrially reared mealworm and cricket samples is largely comprised of Bacillus cereus group members that can pose insect or human health risks. Hepatitis A virus, hepatitis E virus and norovirus genogroup II were not detected in the sample collection, indicating a low food safety risk from these viral pathogens.status: publishe

Consumer acceptance of foods containing edible insects in Belgium two years after their introduction to the market

© 2018 Wageningen Academic Publishers. As the Belgian market and legislation on insect-based foods have evolved the past years, this study aimed at generating an updated and representative insight into the consumer acceptance of these foods. Data were collected via a telephone survey. The distribution of the 388 respondents across regions, gender and age matched that of the Belgian population. Of those surveyed, 79% were aware of the fact that foods with insects can be bought; 11.2% had already eaten foods with processed insects; 31.8% had no experience but were willing to try, and 57% had no experience or interest in tasting such products. Potential consumers accepted invisible processed mealworms in energy shakes (60.7%), energy bars (59.6%), burgers (59.3%), soup (56.8%), sandwich spreads (56.2%), unfried snacks (56.2%) and fried snacks (52.7%). Consumers indicated that the presence of insects should be clearly declared on the package and that they wanted to be able to buy these products primarily in the supermarket.status: publishe