Становище Заславського римо-католицького деканату в ХІХ столітті (The situation of the Zaslavsky Roman-Catholic Deanery in the 19th century)

Стаття присвячена аналізу римо-католицизму на Заславщині у ХІХ ст. Доведено, що становище Заславського римо-католицького деканату протягом ХІХ століття погіршувалося, що відповідало загальним тенденціям ситуації РКЦ в Російській імперії. (The article is devoted to the analysis of Roman Catholicism in Zaslavsk region in the 19th century. It is proved that the situation of Zaslavsky Roman Catholic deanery during the 19th century worsened, which corresponded to the general tendencies of the situation of the RCC in the Russian Empire.

Morphological and injury analysis of bronchioles in saccular stage MV and control lungs after 24 h and 15 d.

<p>Light micrographs show cellular intraluminal debris in MV110+24 h lungs (A, B), intact epithelium of C110+24 h bronchiole (C) and bronchiole with denuded epithelium in MV110+24 h lung (D). The basement membrane perimeter of bronchioles was not different between all saccular stage MV and control lungs (E). Epithelial thickness of bronchioles was greater in MV110+24 h fetuses than in controls; however, it was lower in MV110+15 days fetuses relative to 15 days controls (F). The proportion of bronchioles that contained debris within the lumen was increased in MV110+24 h and MV110+15 d fetuses compared with age-matched controls (G). Values that do not share a common letter are significantly different from each other (P&lt;0.05, scale bar = 10 µm in A and B and 20 µm in C and D).</p

Oligonucleotide primer sequences.

<p>CTGF, Connective tissue growth factor; CYR-61, cysteine rich 61; EGR1, early growth response 1; HSPE1, heat shock 10 kDa protein; uPAR, urokinase plasminogen activator receptor; MT2a, metallothionein 2a; DLK-1, delta like homolog drosophila; F, forward primer; R, reverse primer.</p

The mRNA levels of control and ventilated saccular lungs after 24 h, corrected for the level of housekeeping gene 18 S and expressed as fold change from the mean value in control fetuses ± SE.

<p>IL, interleukin, TNF-α tumor necrosis factor-α; CTGF, connective tissue growth factor; CYR61, cysteine-rich 61; EGR1, early growth response 1.</p

Relative expression of potential repair gene mRNA in saccular and early alveolar stage lungs 24 h after MV.

<p>Metallothionein (A) and Urokinase Plasminogen Activator Receptor (B) mRNA expression was significantly increased in MV saccular and early alveolar stage lungs after 24 h when compared to controls (p&lt;0.05). Relative expression of Delta-Like Homolog Drosophila (C) and Heat Shock 10 kDa Protein (D) mRNA was not different between control and MV lungs at 24 h in the saccular or early-alveolar stage lung.</p

Lung morphometry, collagen and elastin density, percent tissue space and secondary septal crest density in saccular stage MV and control lungs after 24 h and 15 d.

<p>Light micrographs stained with hemotoxylin and eosin depicting lung morphology in C110+24 h (A), MV110+24 h (B), C110+15 d (C) and MV110+15 d (D) lung tissue. At 24 h after MV lung tissue showed signs of heterogeneous injury with regional hypercellularity and atelectasis (arrow, B). Tissue space fraction was increased in MV110+24 h lungs compared to controls (M, p&lt;0.05). Collagen fibres (black staining) are shown in C110 d+24 h (E), MV110+24 h (F), C110+15 d (G) and MV110+15 d (H) and elastin deposits (brown staining) in C110+24 h (I), MV110+24 h (J), C110+15 d (K) and MV110+15 d (L). Collagen fibres (brown staining) were not straight in MV110+24 h lungs (arrow, F), compared to controls at both ages and MV110+15 lungs (E,G,H). Collagen (N) and elastin density (O) was not different between MV lungs and their matched control group. Secondary septal crest density was reduced in MV110+24 h lungs (arrow, J) compared to those in C110+24 h group (I, P). Scale bar = 100 µm for A–D and 20 µm for E–L. Values that do not share a common letter are significantly different.</p

Cell proliferation and myofibroblast density in saccular stage MV and control lungs after 24 h and 15 d.

<p>Immunohistochemical staining using Ki-67 antibody shows proliferating cells labelled brown in C110+24 h (A), MV110+24 h (B), C110+15 d (C) and MV110+15 d lungs (D). The proportion of proliferating cells in the gas-exchanging region was not different between MV lungs and there matched control groups (I). Myofibroblasts were detected using α-SMA antibody, which labelled the myofibroblasts brown as shown in C110+24 h (E), MV110+24 h (F), C110+15 d (G) and MV110+15 d lungs (H). Myofibroblasts were localised at developing septa. α-SMA was increased in MV110+24 h lungs, in comparison to controls (J, p&lt;0.05). Scale bar = 20 µm. Values that do not share a common letter are significantly different.</p

Injury analysis of saccular stage control and ventilated bronchioles after 24 h and 15 d.

<p>Mild injury: 45° bronchiole epithelium detached or absent; moderate: 45°–180° bronchiole epithelium detached or absent; severe: 180° bronchiole epithelium detached or absent. Injured data represent total no. of mild, moderate, and severely injured bronchioles. MV110+24 h lungs had a higher proportion of injured bronchioles relative to all other groups, of which most were classified as severely injured (p&lt;0.05).</p

Haemodynamic effects of prenatal caffeine on the cardiovascular transition in ventilated preterm lambs - Fig 4

<p>Heart rate (<b>a</b>) and peripheral oxygen saturation (<b>b</b>) in caffeine treated (grey dots) and control (black squares) lambs. The figure shows mean± SEM values at different time points during the experiment: before caffeine/ saline infusion; after caffeine/ saline infusion; before cord clamping (CC); after CC; before sustained inflation (SI); after SI; 5, 10, 15, 20, 25 and 30 minutes after ventilation onset. # indicates significant difference compared to “before CC” (grey within caffeine group, black within saline group). ^ indicates significant difference compared to “before SI” (grey within caffeine group, black within saline group).</p

Gestational age, body weight and sex.

<p>Gestational age, body weight and sex.</p