Currents of quasi-trapped particles having discontinuities on the day and night sides of the earth

Currents of quasi-trapped particles having discontinuities on earths day and night side

Currents of quasi-trapped particles and their interaction with the geomagnetic field /symmetrical approximation/

Currents of quasitrapped particles and their interaction with geomagnetic fiel

Manifestly N=2 supersymmetric regularization for N=2 supersymmetric field theories

We formulate the higher covariant derivative regularization for N=2 supersymmetric gauge theories in N=2 harmonic superspace. This regularization is constructed by adding the N=2 supersymmetric higher derivative term to the classical action and inserting the N=2 supersymmetric Pauli--Villars determinants into the generating functional for removing one-loop divergencies. Unlike all other regularization schemes in N=2 supersymmetric quantum field theory, this regularization preserves by construction the manifest N=2 supersymmetry at all steps of calculating loop corrections to the effective action. Together with N=2 supersymmetric background field method this regularization allows to calculate quantum corrections without breaking the manifest gauge symmetry and N=2 supersymmetry. Thus, we justify the assumption about existence of a regularization preserving N=2 supersymmetry, which is a key element of the N=2 non-renormalization theorem. As a result, we give the prove of the N=2 non-renormalization theorem which does not require any additional assumptions.Comment: 15 pages, 3 figures, 2 references added, minor corrections, accepted for publication in Physics Letters

Bacterial Phytochrome as a Scaffold for Engineering of Receptor Tyrosine Kinases Controlled with Near-Infrared Light

Optically controlled receptor tyrosine kinases (opto-RTKs) allow regulation of RTK signaling using light. Until recently, the majority of opto-RTKs were activated with blue-green light. Fusing a photosensory core module of Deinococcus radiodurans bacterial phytochrome (DrBphP-PCM) to the kinase domains of neurotrophin receptors resulted in opto-RTKs controlled with light above 650 nm. To expand this engineering approach to RTKs of other families, here we combined the DrBpP-PCM with the cytoplasmic domains of EGFR and FGFR1. The resultant Dr-EGFR and Dr-FGFR1 opto-RTKs are rapidly activated with near-infrared and inactivated with far-red light. The opto-RTKs efficiently trigger ERK1/2, PI3K/Akt, and PLC gamma signaling. Absence of spectral crosstalk between the opto-RTKs and green fluorescent protein-based biosensors enables simultaneous Dr-FGFR1 activation and detection of calcium transients. Action mechanism of the DrBphP-PCM-based opto-RTKs is considered using the available RTK structures. DrBphP-PCM represents a versatile scaffold for engineering of opto-RTKs that are reversibly regulated with far-red and near-infrared light. (C) 2020 Elsevier Ltd. All rights reserved.Peer reviewe

THE MAIN STAGES OF DESIGN FOR MOBILE AND STATIONARY MULTIFUNCTIONAL TETHERED HIGH-ALTITUDE TELECOMMUNICATION PLATFORMS

This project is an overview the main stages of the development and implementation of mobile and stationary multifunctional tethered high-altitude telecommunication platforms long term use

THE DESIGN OF DEVICE OF INPUT CONTROL FOR THE MODULE OF SECONDARY POWER SUPPLY МП1515,5ВТВ

This project is an overview of main stages for designing a device of input control for module secondary power supply МП1515,5ВТВ installed in on-Board equipment for space and military purposes

DEVELOPMENT OF AN INDICATOR OF FIELD STRENGTH FOR EVALUATION OF ELECTROMAGNETIC RADIATION FROM ENERGY FEEDING LINE FOR MOBILE AND STATIONARY MULTIFUNCTIONAL TETHERED HIGH-ALTITUDE TELECOMMUNICATION PLATFORMS

This project is an overview main stage of designing a device for estimating the electromagnetic radiation transmission line for mobile and stationary multifunctional tethered high-altitude telecommunication platforms

Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing

From a single domain of cyanobacteriochrome (CBCR) we developed a near-infrared (NIR) fluorescent protein (FP), termed miRFP670nano, with excitation at 645 nm and emission at 670 nm. This is the first CBCR-derived NIR FP evolved to efficiently bind endogenous biliverdin chromophore and brightly fluoresce in mammalian cells. miRFP670nano is a monomer with molecular weight of 17 kDa that is 2-fold smaller than bacterial phytochrome (BphP)-based NIR FPs and 1.6-fold smaller than GFP-like FPs. Crystal structure of the CBCR-based NIR FP with biliverdin reveals a molecular basis of its spectral and biochemical properties. Unlike BphP-derived NIR FPs, miRFP670nano is highly stable to denaturation and degradation and can be used as an internal protein tag. miRFP670nano is an effective FRET donor for red-shifted NIR FPs, enabling engineering NIR FRET biosensors spectrally compatible with GFP-like FPs and blue-green optogenetic tools. miRFP670nano unlocks a new source of diverse CBCR templates for NIR FPs.Peer reviewe

Quantum Equivalence of Massive Antisymmetric Tensor Field Models in Curved Space

We study the effective actions for massive rank-2 and rank-3 antisymmetric tensor field models in curved space-time. These models are classically equivalent to massive vector field and massive scalar field with minimal coupling to gravity respectively. We prove that effective action for massive rank-2 antisymmetric tensor field is exactly equal to one for massive vector field and effective action for massive rank-3 antisymmetric tensor field is exactly equal to one for massive scalar field. Prove is based on an identity for mass-dependent zeta-functions associated with Laplacians acting on $p$-forms.Comment: 8 pages, REVTeX fil

Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies

miRFP670nano3 offers improved near-infrared imaging and was used to develop fluorescent nanobodies whose stability and fluorescence strongly depend on antigen binding, with broad implications for detecting and manipulating cellular targets. Small near-infrared (NIR) fluorescent proteins (FPs) are much needed as protein tags for imaging applications. We developed a 17 kDa NIR FP, called miRFP670nano3, which brightly fluoresces in mammalian cells and enables deep-brain imaging. By exploring miRFP670nano3 as an internal tag, we engineered 32 kDa NIR fluorescent nanobodies, termed NIR-Fbs, whose stability and fluorescence strongly depend on the presence of specific intracellular antigens. NIR-Fbs allowed background-free visualization of endogenous proteins, detection of viral antigens, labeling of cells expressing target molecules and identification of double-positive cell populations with bispecific NIR-Fbs against two antigens. Applying NIR-Fbs as destabilizing fusion partners, we developed molecular tools for directed degradation of targeted proteins, controllable protein expression and modulation of enzymatic activities. Altogether, NIR-Fbs enable the detection and manipulation of a variety of cellular processes based on the intracellular protein profile.Peer reviewe