Filogeografía: aplicaciones en taxonomía y conservación

La filogeografía se define como la disciplina que estudia los principios y procesos que gobiernan la distribución geográfica de los linajes genealógicos. Dos de las áreas de estudio donde se utilizan aproximaciones filogeográficas cada vez con mayor frecuencia son la taxonomía y la conservación. En esta revisión presentamos primero un resumen general sobre filogeografía y posteriormente discutimos cómo se han llevado al cabo estudios de taxonomía y conservación empleando aproximaciones filogeográficas, enfatizando sobre todo las limitaciones que deben considerarse. Incluimos ejemplos relevantes de estudios con animales que permitirán a los lectores conocer el sentido y alcance de dichas aplicaciones y diseñar adecuadamente estudios con estos objetivos. Palabras clave: ADN, Biogeografía, Estructura genética, Genealogía.Phylogeography is defined as the discipline that studies the principles and processes that determine the geographical distribution of genealogical lineages. Two of the study areas where phylogeographic approaches are used more and more frequently are taxonomy and conservation. In this review we first present a general description of phylogeography and then discuss how research in taxonomy and conservation has been addressed when using phylogeographic approaches, emphasising in particular the limitations that need to be considered. We include relevant examples of studies with animals in order to help readers acquire the sense and scope of such applications and select the appropriate study design to meet these objectives. Key words: DNA, Biogeography, Genetic structure, Genealogy.La filogeografía se define como la disciplina que estudia los principios y procesos que gobiernan la distribución geográfica de los linajes genealógicos. Dos de las áreas de estudio donde se utilizan aproximaciones filogeográficas cada vez con mayor frecuencia son la taxonomía y la conservación. En esta revisión presentamos primero un resumen general sobre filogeografía y posteriormente discutimos cómo se han llevado al cabo estudios de taxonomía y conservación empleando aproximaciones filogeográficas, enfatizando sobre todo las limitaciones que deben considerarse. Incluimos ejemplos relevantes de estudios con animales que permitirán a los lectores conocer el sentido y alcance de dichas aplicaciones y diseñar adecuadamente estudios con estos objetivos. Palabras clave: ADN, Biogeografía, Estructura genética, Genealogía

Distributional extensions of Carollia castanea and Micronycteris minuta from Guatemala, Central America

Field expeditions in 2011 that inventoried the terrestrial vertebrate fauna of two wildlife protected areas in the tropical Caribbean of Guatemala have produced the first confirmed records of two bats for the country: the white-bellied big-eared bat, Micronycteris (Schizonycteris) minuta (Gervais 1856) and the Chesnut short-tailed bat Carollia castanea H. Allen, 1890, both of neotropical distribution and with their current northern limit at Lancetilla, Honduras. The record of M. minuta at Sierra de Caral, Guatemala extends the range of this species 137 km to the west, and the record of C. castanea at Cerro San Gil extends its range 147 km to the west

Conventional Space-Vector Modulation Techniques versus the Single-Phase Modulator for Multilevel Converters

Space-vector modulation is a well-suited technique to be applied to multilevel converters and is an important research focus in the last 25 years. Recently, a single-phase multilevel modulator has been introduced showing its conceptual simplicity and its very low computational cost. In this paper, some of the most conventional multilevel space-vector modulation techniques have been chosen to compare their results with those obtained with single-phase multilevel modulators. The obtained results demonstrate that the single-phase multilevel modulators applied to each phase are equivalent with the chosen wellknown multilevel space-vector modulation techniques. In this way, single-phase multilevel modulators can be applied to a converter with any number of levels and phases avoiding the use of conceptually and mathematically complex space-vector modulation strategies. Analytical calculations and experimental results are shown validating the proposed concepts

Factores que condicionan el acabado de terneros de raza Tudanca en Cantabria

UnpublishedTomo I. Sección: Sistemas Ganaderos - Economía y Gestión. Sesión: Vacuno carne

Two-dimensional modulation technique with dc voltage control for single-phase two-cell cascaded converters

In this paper, a simple feed-forward modulation technique for single-phase two-cell multilevel cascaded converters is presented. All the possible switching states of the power converter are taken into account applying a two dimensional control region. The proposed technique uses the actual values of the DC-Link capacitor voltages to obtain output phase voltages and currents with low harmonic distortion with any dc voltage in the H-bridges of the cascaded converter. The possible switching sequences of the converter are studied and, depending on the actual dc voltage values, their desired values are achieved. Simulation results are shown in order validate the proposed technique working as a synchronous rectifier

Las exposiciones científicas escolares y su contribución el ámbito afectivo de los alumnos participantes

En este trabajo se analiza la incidencia en el ámbito afectivo de los alumnos de «La Ciencia Divertida», una experiencia innovadora consistente en la planificación, construcción y realización de una exposición científica interactiva a cargo de grupos de alumnos y profesores. Los resultados obtenidos indican un alto grado de implicación y motivación del alumnado que diseñó los módulos y una incidencia positiva de la actividad en la autoestima y desarrollo personal de los alumnos. También sugieren una mejora de la convivencia y el clima de relaciones personales alumno-alumno y alumno-profesor, y en la motivación y satisfacción personal de los alumnos hacia la propia experiencia y la ciencia en general. Sin embargo, no se aprecian grandes logros respecto a la transferencia al aula de las actitudes positivas generadas hacia la experiencia desarrollada y la propia ciencia, aunque sí respecto al clima de relaciones personales.In this paper, the influence of «The Amusing Science» on the students' attitudes is evaluated. This innovative experience includes the design, construction and assembly of a scientific interactive exposition made up by students and teachers. The results show high rates of students' implication and motivation and a positive influence in their self-steem and personal development. They also suggest an improvement of the interpersonal relationships among students and among students and teachers, as well as of the students' satisfaction to the experience and to Science. The improved interpersonal relationships were translated into science classroom without stating the positive attitudes

Do the Historical Biogeography and Evolutionary History of the Digenean Margotrema spp. across Central Mexico Mirror Those of Their Freshwater Fish Hosts (Goodeinae)?

Host-parasite systems provide an ideal platform to study evolution at different levels, including codivergence in a historical biogeography context. In this study we aim to describe biogeographic and codivergent patterns and associated processes of the Goodeinae freshwater fish and their digenean parasite (Margotrema spp.) over the last 6.5 Ma (million years), identifying the main factors (host and/or hydrogeomorphology) that influenced the evolution of Margotrema. We obtained a species tree for Margotrema spp. using DNA sequence data from mitochondrial and nuclear molecular markers (COI and ITS1, respectively) and performed molecular dating to discern divergence events within the genus. The dispersal-extinction-cladogenesis (DEC) model was used to describe the historical biogeography of digeneans and applied to cophylogenetic analyses of Margotrema and their goodeine hosts. Our results showed that the evolutionary history of Margotrema has been shaped in close association with its geographic context, especially with the geological history of central Mexico during the Pleistocene. Host-specificity has been established at three levels of historical association: a) Species-Species, represented by Xenotaenia resolanae -M. resolanae exclusively found in the Cuzalapa River Basin; b) Species-Lineage, represented by Characodon audax-M. bravoae Lineage II, exclusive to the Upper and Middle Mezquital River Basin, and c) Tribe-Lineage, including two instances of historical associations among parasites and hosts at the taxonomical level of tribe, one represented by Ilyodontini-M. bravoae Lineage I (distributed across the Ayuquila and Balsas River Basins), and another comprised of Girardinichthyini/Chapalichthyini-M. bravoae Lineage III, found only in the Lerma River Basin. We show that the evolutionary history of the parasites is, on several occasions, in agreement with the phylogenetic and biogeographic history of their hosts. A series of biogeographic and host-parasite events explain the codivergence patterns observed, in which cospeciation and colonisation via host-switching and vicariant plus dispersal events are appreciated, at different times during the diversification history of both associates, particularly during the Pleistocene.Facultad de Ciencias Naturales y Muse

Automated low-cost device to produce sub-micrometric polymer fibers based on blow spun method

Attending the latest advances in polymeric fibers, the design of low-cost, and high-quality scientific equipment for obtaining fibers seemed essential. To overcome this challenge, a 3D printable prototype was designed, assembled, and validated to obtain fibers using the SBS method. The particular configuration of the prototype consisted of controlling the process conditions such as working distance and injection flow, as well as other parameters such as RPM and the axial movement of the cylindrical collector. Thus, these parameters were automated using a microcontroller (Arduino) that receives information from an Android device with bluetooth connectivity to control each of the elements of the equipment. Subsequently, the repeatability and reproducibility of the fibers was verified using polymers such as polystyrene (PS), polysulfone (PSF) and polyethylene oxide (PEO); furthermore, PSF fibers were manufactured to analyze the influence of working distance and the axial movement of the collector on their production.Fondos de Investigación de Fco. Javier González Benito, política de reinversión de costes generales, Universidad Carlos III de Madrid [2012/00130/004] and Acción Estratégica en Materiales Compuestos Poliméricos e Interfases, Universidad Carlos III de Madrid [2011/00287/002]. Besides, authors greatly appreciate the Consejo Nacional de Ciencia y Tecnología (CONACyT- México) for financial support associated to the scholarship number 625396

Nitrogen Metabolism and Growth Enhancement in Tomato Plants Challenged with Trichoderma harzianum Expressing the Aspergillus nidulans Acetamidase amdS Gene

[EN] Trichoderma is a fungal genus that includes species that are currently being used as biological control agents and/or as biofertilizers. In addition to the direct application of Trichoderma spp. as biocontrol agents in plant protection, recent studies have focused on the beneficial responses exerted on plants, stimulating the growth, activating the defenses, and/or improving nutrient uptake. The amdS gene, encoding an acetamidase of Aspergillus, has been used as a selectable marker for the transformation of filamentous fungi, including Trichoderma spp., but the physiological effects of the introduction of this gene into the genome of these microorganisms still remains unexplored. No evidence of amdS orthologous genes has been detected within the Trichoderma spp. genomes and the amdS heterologous expression in Trichoderma harzianum T34 did not affect the growth of this fungus in media lacking acetamide. However, it did confer the ability for the fungus to use this amide as a nitrogen source. Although a similar antagonistic behavior was observed for T34 and amdS transformants in dual cultures against Rhizoctonia solani, Botrytis cinerea, and Fusarium oxysporum, a significantly higher antifungal activity was detected in amdS transformants against F. oxysporum, compared to that of T34, in membrane assays on media lacking acetamide. In Trichoderma-tomato interaction assays, amdS transformants were able to promote plant growth to a greater extent than the wild-type T34, although compared with this strain the transformants showed similar capability to colonize tomato roots. Gene expression patterns from aerial parts of 3-week-old tomato plants treated with T34 and the amdS transformants have also been investigated using GeneChip Tomato Genome Arrays. The downregulation of defense genes and the upregulation of carbon and nitrogen metabolism genes observed in the microarrays were accompanied by (i) enhanced growth, (ii) increased carbon and nitrogen levels, and (iii) a higher sensitivity to B. cinerea infections in plants treated with amdS transformants as detected in greenhouse assays. These observations suggest that the increased plant development promoted by the amdS transformants was at expense of defensesSIResearch project funding was from the Spanish Ministry of Economy and Competitiveness (Project no. AGL2015-70671-C2) and the Junta de Castilla y León (Projects no. SA230U13 and LE228U14). The grant awarded to Sara Domínguez by Junta de Castilla y León (Spain) and Wagner Bettiol was supported by a fellowship from the Fundação de Amparo à Pesquisa do Estado de São Paulo—FAPESP (Project no. 2014/03426-1