Temporal and Spatial Distribution of Imidacloprid and the Arthropod Fauna Associated with Eastern Hemlock, Tsuga canadensis (L.) Carr

Eastern hemlock, Tsuga canadensis (L.) Carr (Pinales: Pinaceae), is an important component of both the urban and forest landscape of the eastern United States. Eastern hemlock has been heavily impacted by the introduction of the hemlock woolly adelgid, Adelges tsugae (Annand) (Hemiptera: Adelgidae). Two goals of this research were (1) to determine the effect of treatment timing (spring versus fall) and application method (tree injection versus soil injection) on the spatial and temporal distribution of imidacloprid the primary insecticide used to treat A. tsugae and (2) to assess the impact of application method and timing of imidacloprid treatments on the arthropods associated with eastern hemlock. The results of this study showed that xylem fluid concentrations of imidacloprid were significantly (P \u3c 0.05) higher for spring applications than for fall applications, and for trunk injections than soil injections in the first year post treatment. A diverse group of arthropods, making up 393 species, were collected by branch beating the lower crowns of eastern hemlock. No significant (P \u3e 0.05) differences in arthropod abundance were found between imidacloprid treated and control trees and application methods. An extensive literature review revealed 484 native and exotic arthropods from three different taxonomic classes and 21 different orders associated with eastern hemlock in North America. A total of five arthropod species were eastern hemlock dependent, and are likely to experience local extirpation as a result of declining and dying eastern hemlock. In addition, an assessment of the impact of application method and timing of imidacloprid treatments on the spider communities were carried out because spiders are the primary arthropod predator present in the crown of eastern hemlock. No significant (P \u3e 0.05) differences in spider abundance were found between imidacloprid treated and control trees and application methods. This study provides fundamental information to aid the conservation and management of eastern hemlock and biodivisity at risk due to extensive applications of imidacloprid

New Distributional Record for \u3ci\u3eBalcha Indica\u3c/i\u3e (Hymenoptera: Eupelmidae) in Eastern West Virginia Discovered During Emerald Ash Borer Parasitoid Recovery Surveys

Between 2010 and 2012, approximately 6,300 Spathius agrili Yang (Hymenoptera: Braconidae) and 9,500 Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) parasitoids were released for biological control of the invasive emerald ash borer, Agrilus planipennis Fairmaire, at Cacapon State Park and the Cool Front Development in Morgan County, West Virginia. The invasive beetle was first detected there in 2009, and extensive ash mortality is currently occurring. We conducted parasitoid recovery surveys in 2013 but did not recover either of the released parasitoid species. However, we did rear Balcha indica Mani and Kaul (Hymenoptera: Eupelmidae), which is native to Asia and is a documented parasitoid of A. planipennis, from bolts infested with A. planipennis. This is the first documented record of B. indica for West Virginia

Low abundances of heavy elements in the solar outer layers: comparisons of solar models with helioseismic inversions

Recent solar photospheric abundance analyses have led to a significant reduction of the metal abundances compared to the previous determinations. The solar models computed with standard opacities and diffusion processes using these new abundances give poor agreement with helioseismic inversions for the sound-speed profile, the surface helium abundance, and the convective zone depth. We attempt to obtain a good agreement between helioseismic inversions and solar models which present the "old" mixture in the interior and new chemical composition in the convective zone. To reach this result, we assume an undermetallic accretion at the beginning of the main sequence. We compute solar models with the Toulouse-Geneva Evolution Code, in which we simulate an undermetallic accretion in the early stages of the main sequence, in order to obtain new mixture in the outer convective zone. We compare the sound-speed profile, the convective zone depth, and the surface helium abundance with those deduced from helioseismology. The model with accretion but without any mixing process inside is in better agreement with helioseismology than the solar model with the new abundances throughout. There is, however, a spike under the convective zone which reaches 3.4%. Furthermore, the convective zone depth and the surface helium abundance are too low. Introducing undershooting below the convective zone allows us to recover the good convective zone radius and the addition of rotation-induced mixing and tachocline allows us to reconcile the surface helium abundance. But in any case the agreement of the sound-speed profile with helioseismic inference is worse than obtained with the old abundances.Comment: 5 pages, 2 figure

Abundance anomalies in pre-main-sequence stars: Stellar evolution models with mass loss

The effects of atomic diffusion on internal and surface abundances of A and F pre-main-sequence stars with mass loss are studied in order to determine at what age the effects materialize, as well as to further understand the processes at play in HAeBe and young ApBp stars. Self-consistent stellar evolution models of 1.5 to 2.8Msun with atomic diffusion (including radiative accelerations) for all species within the OPAL opacity database were computed and compared to observations of HAeBe stars. Atomic diffusion in the presence of weak mass loss can explain the observed abundance anomalies of pre-main-sequence stars, as well as the presence of binary systems with metal rich primaries and chemically normal secondaries such as V380 Ori and HD72106. This is in contrast to turbulence models which do not allow for abundance anomalies to develop on the pre-main-sequence. The age at which anomalies can appear depends on stellar mass. For A and F stars, the effects of atomic diffusion can modify both the internal and surface abundances before the onset of the MS. The appearance of important surface abundance anomalies on the pre-main-sequence does not require mass loss, though the mass loss rate affects their amplitude. Observational tests are suggested to decipher the effects of mass loss from those of turbulent mixing. If abundance anomalies are confirmed in pre-main-sequence stars they would severely limit the role of turbulence in these stars.Comment: 9 pages, 6 figures, accepeted for publicatio

Looking for pulsations in HgMn stars through CoRoT lightcurves

HgMn Chemically Peculiar stars are among the quietest stars of the main-sequence. However, according to theoretical predictions, these stars could have pulsations related to the very strong overabundances of iron peak elements, which are produced by atomic diffusion in upper layers. Such pulsations have never been detected from ground based observations. Our aim is to search for signatures of pulsations in HgMn stars using the high quality lightcurves provided by the CoRoT satellite. We identified three faint stars (V>12), from VLT-GIRAFFE multiobject spectrograph survey in a field which was planned for observation by CoRoT. They present the typical characteristics of HgMn stars. They were observed by the CoRoT satellite during the long run (131 days) which started from the 24th of October 2007, with the exoplanets CCD's (Additional Programme). In the present work, we present the analysis of the ground based spectra of these three stars and the analysis of the corresponding CoRoT lightcurves. Two of these three HgMn candidates show low amplitude (less than 1.6 mmag) periodic variations (4.3 and 2.53 days respectively, with harmonics) which are compatible with periods predicted by theoretical models.Comment: Accepted paper in A&A (7 May 2009

Choosing an Adequate Pesticide Delivery System for Managing Pathogens with Difficult Biologies: Case Studies on <em>Diplodia corticola, Venturia inaequalis</em> and <em>Erwinia amylovora</em>

With the challenges that negatively impact tree-based agriculture, landscapes and forests, such as climate change, plant pathogen and insect range expansion, invasive species and limited new pesticides, it is important to introduce new and effective tree protection options. In the last 20 years, pathogens that invade wood i.e. vascular tissues of trees causing wilt, yellowing, premature defoliation, cankers and tree death, have been on the rise. Diplodia corticola causes Bot canker of oak species which can kill trees diminishing the valuable ecological services they provide and reducing profits from wood and cork production. Since this and similar pathogens have difficult biologies because they reside in wood and cause severe internal damage and tree death, their management is difficult or inefficient with classical pesticide application methods that cannot reach and distribute the active ingredient in vascular wood tissues. As practical management options for this and other vascular tissue pathogens of trees are limited, we evaluated efficacy of several trunk injected fungicides in control of D. corticola and compared it with the efficacy of trunk injection of similar compounds for control of Venturia inaequalis and Erwinia amylovora, as two well-studied apple tree pathogens with different or partially similar lifestyles to D. corticola, respectively

Spatial and Temporal Distribution of Imidacloprid Within the Crown of Eastern Hemlock

Systemic imidacloprid is the most widely used insecticide to control the hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), an exotic pest of eastern hemlock, Tsuga canadensis (L.) Carriére in the United States. This study was conducted to 1) determine the effect of treatment timing (spring vs. fall) and application method (trunk injection vs. soil injection) on the spatial and temporal distribution of imidacloprid within the crown of A. tsugae-free eastern hemlock using a competitive enzyme-linked immunosorbent assay (ELISA), 2) compare ELISA to gas chromatography-mass spectrometry (GC/MS) for the detection of imidacloprid in xylem fluid, and 3) determine the concentration of imidacloprid in leaf tissue using high performance liquid chromatography with tandem mass spectrometric (LC/MS/MS) detection methods. Xylem fluid concentrations of imidacloprid were found to be significantly higher for spring applications than for fall applications and for trunk injections than soil injections in the first year posttreatment. A total of 69% of samples analyzed by ELISA gave 1.8 times higher concentrations of imidacloprid than those found by GC/MS, leading to evidence of a matrix effect and overestimation of imidacloprid in xylem fluid by ELISA. A comparison of the presence of imidacloprid with xylem fluid and in leaf tissue on the same branch showed significant differences, suggesting that imidacloprid moved intermittently within the crown of eastern hemlock

Chemical composition of A and F dwarf members of the Coma Berenices open cluster

Abundances of 18 chemical elements have been derived for 11 A (normal and Am) and 11 F dwarfs members of the Coma Berenices open cluster in order to set constraints on evolutionary models including transport processes (radiative and turbulent diffusion)calculated with the Montreal code. A spectral synthesis iterative procedure has been applied to derive the abundances from selected high quality lines in high resolution high signal-to-noise echelle spectra obtained with ELODIE at the Observatoire de Haute Provence. The chemical pattern found for the A and F dwarfs in Coma Berenices is reminiscent of that found in the Hyades and the UMa moving group. In graphs representing the abundances [X/H] versus the effective temperature, the A stars often display abundances much more scattered around their mean values than the F stars do. Large star-to-star variations are detected for A stars in their abundances which we interpret as evidence of transport processes competing with radiative diffusion. The F stars have solar abundances for almost all elements except for Mg, Si, V and Ba. The derived abundances patterns, [X/H] versus atomic number, for the slow rotator HD108642 (A2m) and the moderately fast rotator HD106887 (A4m) were compared to the predictions of self consistent evolutionary model codes including radiative and different amounts of turbulent diffusion. None of the models reproduces entirely the overall shape of the abundance pattern. While part of the discrepancies between derived and predicted abundances may be accounted for by non-LTE effects, the inclusion of competing processes such as rotational mixing in the radiative zones of these stars seems necessary to improve the agreement between observed and predicted abundance patterns.Comment: 25 pages, 20 figure

AmFm and lithium gap stars: Stellar evolution models with mass loss

A thorough study of the effects of mass loss on internal and surface abundances of A and F stars is carried out in order to constrain mass loss rates for these stars, as well as further elucidate some of the processes which compete with atomic diffusion. Self-consistent stellar evolution models of 1.3 to 2.5 M_sun stars including atomic diffusion and radiative accelerations for all species within the OPAL opacity database were computed with mass loss and compared to observations as well as previous calculations with turbulent mixing. Models with unseparated mass loss rates between 5 x 10^-14 and 10^-13 M_sun/yr reproduce observations for many cluster AmFm stars as well as Sirius A and o Leonis. These models also explain cool Fm stars, but not the Hyades lithium gap. Like turbulent mixing, these mass loss rates reduce surface abundance anomalies; however, their effects are very different with respect to internal abundances. For most of the main sequence lifetime of an A or F star, surface abundances in the presence of such mass loss depend on separation which takes place between log(Delta M/M_star)= -6 and -5. The current observational constraints do not allow us to conclude that mass loss is to be preferred over turbulent mixing (induced by rotation or otherwise) in order to explain the AmFm phenomenon. Internal concentration variations which could be detectable through asteroseismic tests should provide further information. If atomic diffusion coupled with mass loss are to explain the Hyades Li gap, the wind would need to be separated.Comment: 27 pages, 25 figures, accepted for publication in A&

Loss of hepatic DEPTOR alters the metabolic transition to fasting

Objective The mechanistic target of rapamycin (mTOR) is a serine/threonine kinase that functions into distinct protein complexes (mTORC1 and mTORC2) that regulates growth and metabolism. DEP-domain containing mTOR-interacting protein (DEPTOR) is part of these complexes and is known to reduce their activity. Whether DEPTOR loss affects metabolism and organismal growth in vivo has never been tested. Methods We have generated a conditional transgenic mouse allowing the tissue-specific deletion of DEPTOR. This model was crossed with CMV-cre mice or Albumin-cre mice to generate either whole-body or liver-specific DEPTOR knockout (KO) mice. Results Whole-body DEPTOR KO mice are viable, fertile, normal in size, and do not display any gross physical and metabolic abnormalities. To circumvent possible compensatory mechanisms linked to the early and systemic loss of DEPTOR, we have deleted DEPTOR specifically in the liver, a tissue in which DEPTOR protein is expressed and affected in response to mTOR activation. Liver-specific DEPTOR null mice showed a reduction in circulating glucose upon fasting versus control mice. This effect was not associated with change in hepatic gluconeogenesis potential but was linked to a sustained reduction in circulating glucose during insulin tolerance tests. In addition to the reduction in glycemia, liver-specific DEPTOR KO mice had reduced hepatic glycogen content when fasted. We showed that loss of DEPTOR cell-autonomously increased oxidative metabolism in hepatocytes, an effect associated with increased cytochrome c expression but independent of changes in mitochondrial content or in the expression of genes controlling oxidative metabolism. We found that liver-specific DEPTOR KO mice showed sustained mTORC1 activation upon fasting, and that acute treatment with rapamycin was sufficient to normalize glycemia in these mice. Conclusion We propose a model in which hepatic DEPTOR accelerates the inhibition of mTORC1 during the transition to fasting to adjust metabolism to the nutritional status. Keywords: DEPTOR; mTOR; Liver; Glucose; Fastin