PARASITOLOGICAL ANALYSIS OF CRESS (NASTURTIUM OFFICINALE, BARBAREA VERNA E LEPIDIUM SATIVUM) SAMPLES FROM CURITIBA AREA, BRAZIL

Food-borne parasite infections affect communities worldwide by transmitting microorganisms, imposing a serious risk to human and animal health. The aim of this study was to analyze the parasitological contamination in cress (Nasturtium officinale, Barbarea verna e Lepidium sativum) from various establishments in the metropolitan area of Curitiba, State of Parana. We analyzed 50 cress samples using the wash and centrifugation technique and the sedimentation method. Among the samples, 6 presented trophozoites of Balantidium coli, cyst of Entamoeba coli and parasites of Stronylidae and Trichuridae Family and 14 samples were contaminated with maggots, slugs and snails. Factors related to this parasitological contamination may be the failure in the quality of water used for irrigation, and the planting, storage and the distribution of the plants. As these vegetables are mainly eaten in natura, we recommend the inclusion of safety procedures along the entire process to protect the health of consumers, since vegetables  can be a zoonosis transmitter vehicle. Food-borne parasite infections affect communities worldwide by transmitting microorganisms, imposing a serious risk to human and animal health. The aim of this study was to analyze the parasitological contamination in cress (Nasturtium officinale, Barbarea verna e Lepidium sativum) from various establishments in the metropolitan area of Curitiba, State of Parana. We analyzed 50 cress samples using the wash and centrifugation technique and the sedimentation method. Among the samples, 6 presented trophozoites of Balantidium coli, cyst of Entamoeba coli and parasites of Stronylidae and Trichuridae Family and 14 samples were contaminated with maggots, slugs and snails. Factors related to this parasitological contamination may be the failure in the quality of water used for irrigation, and the planting, storage and the distribution of the plants. As these vegetables are mainly eaten in natura, we recommend the inclusion of safety procedures along the entire process to protect the health of consumers, since vegetables can be a zoonosis transmitter vehicle

User Value in Modern Payment Platforms: A Graph Approach

Payment platforms have significantly evolved in recent years to keep pace with the proliferation of online and cashless payments. These platforms are increasingly aligned with online social networks, allowing users to interact with each other and transfer small amounts of money in a Peer-to-Peer fashion. This poses new challenges for analysing payment data, as traditional methods are only user-centric or business-centric and neglect the network users build during the interaction. This paper proposes a first methodology for measuring user value in modern payment platforms. We combine quantitative user-centric metrics with an analysis of the graph created by users’ activities and its topological features inspired by the evolution of opinions in social networks. We showcase our approach using a dataset from a large operational payment platform and show how it can support business decisions and marketing campaign design, e.g., by targeting specific users

Varicella seroepidemiology and immunization in a cohort of future healthcare workers in the pre-vaccination era

Objectives: The goal of this study was to establish the seroprevalence of positive antibodies against varicella and compliance with varicella vaccination in the pre-vaccination era. Methods: A cohort of 10 683 Italian students from Padua University Medical School (from 2004 to 2019) were enrolled and classified as unvaccinated, vaccinated once, or vaccinated twice against varicella, according to their vaccination certificate. The antibody titre was measured and the seroprevalence of positive subjects was determined. Subjects with negative or equivocal antibodies were invited for vaccination, and then the antibody titre was retested. Results: Unvaccinated students were mostly seropositive (95.6%), compared with vaccinated students who were less seropositive (68.0% after one dose and 78.6% after two doses) and had significantly lower antibody titres (p < 0.0001). The post-test vaccination had a positive response rate of 85.4%: 67.4% after one dose and 91.4% after two doses. Conclusions: In the pre-vaccination era, only 3.3% of future healthcare workers were vaccinated against varicella (1.1% once and 2.2% twice). Vaccination or revaccination of negative and equivocal individuals could reduce the number of susceptible people. Implementation of varicella vaccine (two doses) in healthcare workers is of primary importance to reduce the risk of transmission

Acute renal failure and renal replacement therapy in the postoperative period of orthotopic liver transplant patients versus nonelective abdominal surgery patients

Acute renal failure (ARF) often complicates the postoperative period of patients undergoing orthotopic liver transplantation (OLT); it is habitually associated with high mortality rates. Similarly, patients undergoing major nonelective abdominal surgery are prone to ARF because of their frequent preexistent morbidities, abdominal sepsis, and needed for extended surgical procedures. The aim of this study was to evaluate the incidence of ARF and use of renal replacement therapy (RRT) among OLT versus nonelective abdominal surgery patients and associations with clinical outcomes. We studied all the patients admitted to a surgical intensive care unit (ICU) from January 2008 to December 2009 after OLT or nonelective abdominal surgery. The inclusion criteria were an ICU stay of at least 48 hours and without prior end-stage renal failure. OLT patients (n = 84) were younger and less severly ill than surgery patients (n = 60). ARF occurrence was lower among the OLT (29%) than the surgery group (47%) requiring RRT in 71% and 53% of patients due to ARF, respectively. The ICU mortality of ARF patients in both groups (29% OLT and 51% surgery) were greater than among subjects without ARF (2% and 6%). The occurrence of ARF is common among these two patient groups, and associated with increased risk of death among in surgery (+45%) versus in OLT (+27%) patients. © 2011 Elsevier Inc. All rights reserved

Nitrate sensing by the maize root apex transition zone: A merged transcriptomic and proteomic survey

Nitrate is an essential nutrient for plants, and crops depend on its availability for growth and development, but its presence in agricultural soils is far from stable. In order to overcome nitrate fluctuations in soil, plants have developed adaptive mechanisms allowing them to grow despite changes in external nitrate availability. Nitrate can act as both nutrient and signal, regulating global gene expression in plants, and the root tip has been proposed as the sensory organ. A set of genome-wide studies has demonstrated several nitrate-regulated genes in the roots of many plants, although only a few studies have been carried out on distinct root zones. To unravel new details of the transcriptomic and proteomic responses to nitrate availability in a major food crop, a double untargeted approach was conducted on a transition zone-enriched root portion of maize seedlings subjected to differing nitrate supplies. The results highlighted a complex transcriptomic and proteomic reprogramming that occurs in response to nitrate, emphasizing the role of this root zone in sensing and transducing nitrate signal. Our findings indicated a relationship of nitrate with biosynthesis and signalling of several phytohormones, such as auxin, strigolactones, and brassinosteroids. Moreover, the already hypothesized involvement of nitric oxide in the early response to nitrate was confirmed with the use of nitric oxide inhibitors. Our results also suggested that cytoskeleton activation and cell wall modification occurred in response to nitrate provision in the transition zone

INFLUENCE OF ANATOMY ON THE MECHANICAL RESISTANCE OF WOODS IN AN AGROFORESTRY SYSTEM

This study aimed to evaluate the influence of anatomy on the mechanical resistance of Parapiptadenia rigida (Benth.) Brenan, Peltophorum dubium (Spreng.) Taub., Eucalyptus grandis × Eucalyptus urophylla, and Schizolobium parahyba (Vell.) Blake from an agroforestry system. For this purpose, twelve 9-year-old trees were felled. To evaluate the anatomical properties, the specimens were taken from the diameter region at breast height (DBH at 1.30 m from the ground). To determine the mechanical characteristics, central planks from the DBH of the tree logs were removed to produce test specimens with dimensions of 2.5 × 2.5 × 41.0 cm. The modulus of elasticity, modulus of rupture, tension of the proportional limit, and maximum force were then evaluated. Conversely, test specimens with dimensions of 5 × 5 × 15 cm for were used for the transversal and longitudinal Janka hardness and perpendicular compression tests. The higher the cell wall fraction, vessel frequency, and ray frequency values, the better the mechanical properties. Conversely, with the increase in the fiber diameter, lumen diameter, vessel diameter, height, and width of rays, the values of the mechanical properties of wood are lower

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

In several cultivation areas, grapevine can suffer from Fe chlorosis due to the calcareous and alkaline nature of soils. This plant species has been described to cope with Fe deficiency by activating Strategy I mechanisms, hence increasing root H+ extrusion and ferric-chelate reductase activity. The degree of tolerance exhibited by the rootstocks has been reported to depend on both reactions, but to date, little emphasis has been given to the role played by root exudate extrusion. We studied the behaviour of two hydroponically-grown, tolerant grapevine rootstocks (Ramsey and 140R) in response to Fe deficiency. Under these experimental conditions, the two varieties displayed differences in their ability to modulate morpho-physiological parameters, root acidification and ferric chelate reductase activity. The metabolic profiling of root exudates revealed common strategies for Fe acquisition, including ones targeted at reducing microbial competition for this micronutrient by limiting the exudation of amino acids and sugars and increasing instead that of Fe(III)-reducing compounds. Other modifications in exudate composition hint that the two rootstocks cope with Fe shortage via specific adjustments of their exudation patterns. Furthermore, the presence of 3-hydroxymugenic acid in these compounds suggests that the responses of grapevine to Fe availability are rather diverse and much more complex than those usually described for Strategy I plants